##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631664.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29637
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32678746161892	33.0	31.0	34.0	31.0	34.0
2	32.505078111819685	34.0	31.0	34.0	31.0	34.0
3	32.59925093632959	34.0	31.0	34.0	31.0	34.0
4	36.12352802240443	37.0	35.0	37.0	35.0	37.0
5	36.01255187772042	37.0	35.0	37.0	35.0	37.0
6	36.050747376590074	37.0	35.0	37.0	35.0	37.0
7	36.04622600128218	37.0	35.0	37.0	35.0	37.0
8	36.01484630698114	37.0	35.0	37.0	35.0	37.0
9	37.813206464891856	39.0	38.0	39.0	35.0	39.0
10	37.703681209299184	39.0	37.0	39.0	35.0	39.0
11	37.8045348719506	39.0	37.0	39.0	35.0	39.0
12	37.71059823868813	39.0	37.0	39.0	35.0	39.0
13	37.77264905354793	39.0	37.0	39.0	35.0	39.0
14	39.08526504032122	40.0	38.0	41.0	36.0	41.0
15	39.082633194992745	40.0	38.0	41.0	36.0	41.0
16	39.039207747072915	40.0	38.0	41.0	36.0	41.0
17	39.006613354927964	40.0	38.0	41.0	36.0	41.0
18	38.97860782130445	40.0	38.0	41.0	36.0	41.0
19	39.00199075479974	40.0	39.0	41.0	36.0	41.0
20	38.991024732597765	40.0	39.0	41.0	36.0	41.0
21	38.97317542261362	40.0	38.0	41.0	36.0	41.0
22	38.94469750649526	40.0	38.0	41.0	35.0	41.0
23	38.9144312852178	40.0	38.0	41.0	35.0	41.0
24	38.8981678307521	40.0	38.0	41.0	35.0	41.0
25	38.82359887977866	40.0	38.0	41.0	35.0	41.0
26	38.742281607450145	40.0	38.0	41.0	35.0	41.0
27	38.636130512535004	40.0	38.0	41.0	35.0	41.0
28	38.6090697439012	40.0	38.0	41.0	35.0	41.0
29	38.6026588386139	40.0	38.0	41.0	35.0	41.0
30	38.55488072342005	40.0	38.0	41.0	35.0	41.0
31	38.49502311300064	40.0	38.0	41.0	34.0	41.0
32	38.435536660255764	40.0	38.0	41.0	34.0	41.0
33	38.34213989270169	40.0	38.0	41.0	34.0	41.0
34	38.35222863312751	40.0	38.0	41.0	34.0	41.0
35	38.23808077740662	40.0	38.0	41.0	34.0	41.0
36	38.201099976380874	40.0	38.0	41.0	34.0	41.0
37	38.14664102304552	40.0	37.0	41.0	33.0	41.0
38	38.07774066201033	40.0	37.0	41.0	33.0	41.0
39	38.02341667510207	40.0	37.0	41.0	33.0	41.0
40	37.734116138610524	40.0	37.0	41.0	33.0	41.0
41	37.810473394743056	40.0	37.0	41.0	33.0	41.0
42	37.82329520531768	40.0	37.0	41.0	33.0	41.0
43	37.28407058744138	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	1.0
22	7.0
23	11.0
24	19.0
25	29.0
26	71.0
27	91.0
28	129.0
29	202.0
30	269.0
31	344.0
32	486.0
33	690.0
34	930.0
35	1428.0
36	2106.0
37	3686.0
38	8432.0
39	10703.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.09478017343186	16.29044775112191	12.649728380065458	27.965043695380775
2	21.13574248405709	18.983027971792016	32.3750717009144	27.506157843236494
3	21.2977021965786	19.809697337787224	28.84907379289402	30.043526672740157
4	15.966528326078889	15.399669332253602	33.20511522758714	35.42868711408037
5	16.806694334784222	33.23885683436245	32.99929142625772	16.955157404595607
6	35.45568039950062	34.12626109255323	14.434659378479603	15.983399129466545
7	31.13338057158282	27.290211559874482	19.40479805648345	22.171609812059252
8	27.88068967844249	31.076019840064784	19.273205790059723	21.770084691433006
9	28.100010122482033	12.946654519688227	17.046259742888957	41.90707561494078
10	19.556635286972366	23.389681816648107	29.577892499240814	27.47579039713871
11	40.12551877720417	18.628741100651215	18.193474373249654	23.05226574889496
12	24.01390154199143	23.5280224044269	25.539022168235654	26.919053885346024
13	34.12963525323076	17.5118939163883	20.457536187873266	27.900934642507675
14	23.81145190133954	18.92229307959645	23.177109693963626	34.08914532510038
15	29.77696797921517	23.18385801531869	20.32594392144954	26.713230084016597
16	26.669365995208693	22.944292607213956	21.989405135472552	28.3969362621048
17	26.652495191821036	23.288456996322164	21.722846441947567	28.336201369909237
18	26.50065796133212	21.50690015858555	23.36606269190539	28.62637918817694
19	28.272092317036133	21.77345885211054	22.5630124506529	27.391436380200425
20	28.808583864763644	20.757836488173567	23.123123123123122	27.31045652393967
21	28.153996693322537	21.695853156527313	21.452913587745048	28.697236562405102
22	29.422681108074368	22.74859128791713	20.676856631912813	27.151870972095693
23	28.757971454600668	22.758713769949726	21.105375037959305	27.377939737490298
24	28.474541957688025	21.469784391132706	21.952289368019706	28.103384283159567
25	28.410432904814925	21.213348179640317	22.60012821810575	27.77609069743901
26	28.123629247224752	22.525896683200052	22.19185477612444	27.15861929345075
27	28.14050005061241	21.77345885211054	22.951040928569018	27.135000168708036
28	26.58838613894794	21.75658804872288	22.525896683200052	29.129129129129126
29	27.830077268279513	22.670985592333906	22.21209974018963	27.28683739919695
30	26.78408745824476	21.301076357256132	24.148867969092688	27.765968215406417
31	27.42180382629821	22.05351418834565	22.627121503526	27.897560481830148
32	25.94729561021696	21.952289368019706	22.853190268920606	29.247224752842733
33	26.67274015588622	21.878057833114013	23.632621385430376	27.816580625569387
34	27.14512265074063	21.84769038701623	23.845193508114857	27.161993454128286
35	26.25097007119479	22.971285892634207	24.15899045112528	26.618753585045717
36	26.635624388433378	21.959037689374767	23.743968687788914	27.661369234402944
37	25.920302324796708	21.473158551810236	24.506529000911023	28.100010122482033
38	26.254344231872324	20.737591524108378	25.552518810945777	27.455545433073524
39	24.52339980429868	20.67010831055775	27.040523669737155	27.765968215406417
40	24.894557478827142	21.186354894220063	27.772716536761482	26.146371090191316
41	23.18723217599622	20.359685528224855	28.454296993622837	27.998785302156087
42	21.49677767655296	21.29432803590107	29.07176839761109	28.13712588993488
43	21.628369942976686	20.261834868576443	29.517157607045245	28.592637581401625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.5
4	1.0
5	1.0
6	2.0
7	3.0
8	3.5
9	4.0
10	4.5
11	5.0
12	5.0
13	11.5
14	18.0
15	29.5
16	41.0
17	37.0
18	33.0
19	33.0
20	41.0
21	49.0
22	40.0
23	31.0
24	32.5
25	34.0
26	34.0
27	49.0
28	64.0
29	76.0
30	88.0
31	138.0
32	188.0
33	188.0
34	264.0
35	340.0
36	429.0
37	518.0
38	662.5
39	807.0
40	807.0
41	931.0
42	1055.0
43	1265.5
44	1476.0
45	1751.0
46	2026.0
47	2026.0
48	2186.0
49	2346.0
50	2660.0
51	2974.0
52	3143.5
53	3313.0
54	3313.0
55	3125.5
56	2938.0
57	2710.0
58	2482.0
59	2372.5
60	2263.0
61	2263.0
62	2118.5
63	1974.0
64	1636.0
65	1298.0
66	1106.0
67	914.0
68	914.0
69	778.5
70	643.0
71	575.5
72	508.0
73	532.5
74	557.0
75	557.0
76	474.0
77	391.0
78	262.5
79	134.0
80	94.0
81	54.0
82	54.0
83	44.0
84	34.0
85	25.0
86	16.0
87	11.5
88	7.0
89	7.0
90	6.0
91	5.0
92	4.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	29637.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.2356513817188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5313257182416	58.362857239261736
2	7.0019284972556	9.555623038769106
3	2.6009988626811054	5.32442554914465
4	1.409286456015428	3.8465431723858687
5	0.7713989022400237	2.631845328474542
6	0.6181080947436087	2.5306205081485977
7	0.4252583691836028	2.0312447278739416
8	0.2966918854769322	1.6195971252151025
9	0.2521881026553924	1.5487397509869418
>10	1.0878702467487515	12.359550561797752
>50	0.0049448647579488705	0.18895299794176199
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	56	0.18895299794176199	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	49	0.16533387319904175	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	47	0.1585855518439788	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	43	0.14508890913385294	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	41	0.13834058777879002	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	41	0.13834058777879002	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	41	0.13834058777879002	No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT	41	0.13834058777879002	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	41	0.13834058777879002	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	40	0.13496642710125856	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	39	0.1315922664237271	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	39	0.1315922664237271	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	34	0.11472146303606978	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	32	0.10797314168100684	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	30	0.1012248203259439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.003374160677531464	0.0
25	0.0	0.0	0.0	0.006748321355062928	0.0
26	0.0	0.0	0.0	0.013496642710125855	0.0
27	0.0	0.0	0.0	0.03374160677531464	0.0
28	0.0	0.0	0.0	0.11472146303606978	0.0
29	0.0	0.0	0.0	0.28680365759017445	0.0
30	0.0	0.0	0.0	0.516246583662314	0.0
31	0.0	0.0	0.0	1.1303438269730404	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTGAC	30	3.5161883E-4	30.833332	5
TGACCAT	30	3.5161883E-4	30.833332	8
AGTGACC	30	3.5161883E-4	30.833332	6
TTAGTGA	30	3.5161883E-4	30.833332	4
TTATGAG	25	0.005411337	29.6	3
GGTATCA	45	3.8377148E-6	28.777777	1
TCTTATA	65	2.746674E-9	28.461536	37
AGGCGGC	35	8.6660794E-4	26.428572	8
TGTGGGG	35	8.6660794E-4	26.428572	18
GTTGGGT	35	8.6660794E-4	26.428572	19
GTGTGGG	35	8.6660794E-4	26.428572	17
GAGGCGG	40	0.0018873345	23.125	7
TTGGGTT	40	0.0018873345	23.125	20
GTGACCA	40	0.0018873345	23.125	7
TGCAGGA	40	0.0018873345	23.125	2
GGTGTGG	40	0.0018873345	23.125	16
GGCGGCG	40	0.0018873345	23.125	9
GCCTGTC	60	3.5710007E-5	21.583332	37
CTTTAGT	45	0.0037397526	20.555555	2
GGCTGCC	45	0.0037397526	20.555555	23
>>END_MODULE