FastQCFastQC Report
Fri 10 Feb 2017
ERR1631663.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631663.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412866
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5270.12764432043326407No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4900.11868257497590017No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4300.10415001477476955No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4270.103423386764713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAA250.005492314729.622
GGTAAGT250.005492314729.628
TTCGCTA250.005492314729.626
GGTATCA4350.029.344831
TACGGCT556.2454274E-726.9090930
TCGTTGG451.3212065E-424.6666662
GTACCTA551.898833E-523.5454541
TATATCT551.898833E-523.5454545
CTATCGC400.001929102823.12500232
TATTGCG400.001929102823.12500211
TATCGCC400.001929102823.12500233
GTATCAA5700.022.3947372
TAGAATA753.7287646E-722.24
GCCTATA502.6980398E-422.237
TATACAC1350.021.92592637
ATTAGAG705.088865E-621.1428593
GGGTACT705.088865E-621.1428591
CTTATAC5000.021.0937
GAGTATA450.003821718320.5555551
GGCCCTA450.003821718320.55555511
Produced by FastQC (version 0.11.5)