##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631663.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412866
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.619840335605254	34.0	31.0	34.0	31.0	34.0
2	32.79131970179186	34.0	31.0	34.0	31.0	34.0
3	32.883376204385925	34.0	31.0	34.0	31.0	34.0
4	36.30389036636584	37.0	37.0	37.0	35.0	37.0
5	36.21477428511914	37.0	35.0	37.0	35.0	37.0
6	36.26730222396613	37.0	37.0	37.0	35.0	37.0
7	36.2480126723925	37.0	37.0	37.0	35.0	37.0
8	36.23452403443248	37.0	37.0	37.0	35.0	37.0
9	38.07381813954164	39.0	38.0	39.0	37.0	39.0
10	37.99796544157184	39.0	38.0	39.0	35.0	39.0
11	38.07023828554543	39.0	38.0	39.0	37.0	39.0
12	38.01026483168873	39.0	38.0	39.0	35.0	39.0
13	38.05794858380201	39.0	38.0	39.0	35.0	39.0
14	39.47105114007935	41.0	39.0	41.0	37.0	41.0
15	39.47042866208407	41.0	39.0	41.0	37.0	41.0
16	39.42287812510597	41.0	39.0	41.0	37.0	41.0
17	39.391533814845495	40.0	39.0	41.0	37.0	41.0
18	39.40272630829373	41.0	39.0	41.0	37.0	41.0
19	39.43056827154573	41.0	39.0	41.0	37.0	41.0
20	39.41304927022327	41.0	39.0	41.0	37.0	41.0
21	39.38150877040008	40.0	39.0	41.0	37.0	41.0
22	39.36364825391289	40.0	39.0	41.0	36.0	41.0
23	39.29519262908546	40.0	39.0	41.0	36.0	41.0
24	39.309378829935135	40.0	39.0	41.0	36.0	41.0
25	39.25234337533243	40.0	39.0	41.0	36.0	41.0
26	39.20075036452505	40.0	39.0	41.0	36.0	41.0
27	39.10153657603193	40.0	39.0	41.0	36.0	41.0
28	39.094706272737405	40.0	39.0	41.0	36.0	41.0
29	39.06878987371205	40.0	39.0	41.0	35.0	41.0
30	39.04049982318718	40.0	39.0	41.0	35.0	41.0
31	38.95788221844376	40.0	39.0	41.0	35.0	41.0
32	38.910687729190585	40.0	38.0	41.0	35.0	41.0
33	38.86375482602104	40.0	38.0	41.0	35.0	41.0
34	38.831909142433624	40.0	38.0	41.0	35.0	41.0
35	38.7797566280585	40.0	38.0	41.0	35.0	41.0
36	38.73963707352991	40.0	38.0	41.0	35.0	41.0
37	38.69804004204754	40.0	38.0	41.0	35.0	41.0
38	38.6525022646573	40.0	38.0	41.0	35.0	41.0
39	38.60375763564934	40.0	38.0	41.0	35.0	41.0
40	38.30773422853904	40.0	38.0	41.0	34.0	41.0
41	38.39087500544971	40.0	38.0	41.0	34.0	41.0
42	38.38588549311399	40.0	38.0	41.0	34.0	41.0
43	37.91508867283816	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	1.0
16	2.0
17	7.0
18	3.0
19	13.0
20	19.0
21	35.0
22	67.0
23	143.0
24	220.0
25	358.0
26	555.0
27	774.0
28	1318.0
29	1817.0
30	2608.0
31	3654.0
32	5047.0
33	6983.0
34	10112.0
35	15154.0
36	23782.0
37	41415.0
38	95696.0
39	203082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.018558079376845	16.752893190526706	12.266691856437681	27.961856873658764
2	20.390393008869705	19.5312764916462	32.71230859407168	27.366021905412406
3	20.487276743543912	20.206071703652032	28.99754399732601	30.30910755547805
4	15.608696284024356	15.162062267176275	33.52661638400837	35.702625064791
5	16.772996565471608	33.468001724530474	33.487620680802	16.271381029195915
6	35.89033730072227	33.65014314571798	14.679339059162052	15.780180494397698
7	31.095561271695903	28.078843983277867	19.741998614562593	21.083596130463636
8	27.940300242693755	31.73862706059593	19.464184505384313	20.856888191325996
9	28.104033754293162	13.097469881268983	17.47903678190987	41.31945958252799
10	18.33887992714343	24.211971923093692	30.867642285874837	26.58150586388804
11	38.93127552280885	19.620651736883154	19.15270329840675	22.295369441901247
12	23.35697296459384	23.727795459059354	26.972916151971827	25.94231542437498
13	33.84923922047347	17.82103636530981	21.44812118217533	26.88160323204139
14	24.258960534410683	19.244016218337183	22.979610818037813	33.517412429214325
15	28.512640905281618	24.66732547606245	20.224964031913505	26.595069586742433
16	26.337358852509045	24.25799169706394	21.85018868107328	27.55446076935374
17	26.530641903184083	23.77623732639646	22.260975716091906	27.432145054327556
18	26.265180470176762	22.44311713727941	23.71689603890851	27.574806353635317
19	27.996492808804796	22.44093725324924	23.331298774905175	26.23127116304079
20	28.896300494591465	21.76711087859015	23.072861412661734	26.263727214156653
21	27.187029205601814	22.77058416047822	22.124611859537964	27.917774774382003
22	27.8928272127034	23.28794330363847	21.538707474095713	27.280522009562425
23	27.625185895665904	22.788992070066318	22.416716319580686	27.169105714687092
24	27.68694927652071	22.344053518575034	22.423255971671196	27.545741233233056
25	27.712623466209372	22.403152596726297	22.855841846991517	27.028382090072807
26	27.419065750146537	22.848575566890954	22.59522460071791	27.1371340822446
27	27.018451507268704	22.411629923510294	23.32572796016141	27.244190609059597
28	26.596522842762543	22.917363018509636	22.388862245861855	28.097251892865966
29	26.916481376524104	23.458943095338437	22.80134474623728	26.823230781900183
30	26.709876812331363	23.012551287827044	23.247252135075303	27.030319764766293
31	27.18048955351131	23.088120600872923	22.7909297447598	26.94046010085597
32	25.274544283133025	22.863592545765453	23.19396608100449	28.66789709009703
33	25.75290772308691	22.529101451802763	24.438922071568015	27.27906875354231
34	26.410748281524754	21.985825909617162	24.306191355064357	27.297234453793724
35	25.830899129499645	23.17507375274302	24.395324390964625	26.598702726792713
36	25.828477036132792	22.435366438505472	24.794969796495717	26.941186728866022
37	26.113799634748318	21.87537845208857	24.76130269869643	27.249519214466677
38	25.46806954314475	21.557842011693868	25.842040758987178	27.132047686174204
39	24.862061782757603	20.838238072401218	26.676209714532074	27.623490430309104
40	24.428749279427223	21.438917227381282	27.539443790479233	26.59288970271226
41	23.239985854974737	20.753222595224603	28.387660887551892	27.61913066224877
42	22.474604351048523	20.964913555487737	29.112593432251625	27.44788866121211
43	21.627113881985924	21.03588089113659	29.328644160575102	28.008361066302385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	4.5
2	3.0
3	12.5
4	22.0
5	22.0
6	25.0
7	28.0
8	29.5
9	31.0
10	45.5
11	60.0
12	60.0
13	115.0
14	170.0
15	323.0
16	476.0
17	513.0
18	550.0
19	550.0
20	653.5
21	757.0
22	815.0
23	873.0
24	1126.0
25	1379.0
26	1379.0
27	1667.5
28	1956.0
29	2682.5
30	3409.0
31	4181.5
32	4954.0
33	4954.0
34	6047.5
35	7141.0
36	8243.0
37	9345.0
38	11219.0
39	13093.0
40	13093.0
41	15062.5
42	17032.0
43	18584.0
44	20136.0
45	23581.0
46	27026.0
47	27026.0
48	29400.5
49	31775.0
50	35450.5
51	39126.0
52	41095.5
53	43065.0
54	43065.0
55	40826.0
56	38587.0
57	37054.5
58	35522.0
59	33181.0
60	30840.0
61	30840.0
62	28382.5
63	25925.0
64	22134.5
65	18344.0
66	15913.5
67	13483.0
68	13483.0
69	11495.5
70	9508.0
71	8200.0
72	6892.0
73	5624.5
74	4357.0
75	4357.0
76	3514.0
77	2671.0
78	2205.5
79	1740.0
80	1411.0
81	1082.0
82	1082.0
83	877.0
84	672.0
85	529.5
86	387.0
87	316.0
88	245.0
89	245.0
90	188.0
91	131.0
92	92.5
93	54.0
94	33.0
95	12.0
96	12.0
97	6.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	412866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.27731803319973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.74681574687742	58.58431433975895
2	5.144977103147171	6.717006132713371
3	1.5894729556804437	3.112695948993669
4	0.8134262194180751	2.12393128085988
5	0.5189004313525218	1.6936214242481546
6	0.3385048465651297	1.325801311500687
7	0.2651421720815031	1.2115438923684352
8	0.1872283364221895	0.9777410929166542
9	0.16830138907337128	0.9887636969974569
>10	1.0796052636812483	13.737521911359165
>50	0.10614089149398	4.782192771058346
>100	0.04111090867359617	4.616296888472164
>500	3.737355333963288E-4	0.12856930875302983
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	527	0.12764432043326407	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	490	0.11868257497590017	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	430	0.10415001477476955	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	427	0.103423386764713	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.4220933668551056E-4	0.0
10	0.0	0.0	0.0	2.4220933668551056E-4	0.0
11	0.0	0.0	0.0	2.4220933668551056E-4	0.0
12	0.0	0.0	0.0	2.4220933668551056E-4	0.0
13	0.0	0.0	0.0	2.4220933668551056E-4	0.0
14	0.0	0.0	0.0	2.4220933668551056E-4	0.0
15	2.4220933668551056E-4	0.0	0.0	4.8441867337102113E-4	0.0
16	2.4220933668551056E-4	0.0	0.0	7.266280100565317E-4	0.0
17	2.4220933668551056E-4	0.0	0.0	0.0012110466834275527	0.0
18	2.4220933668551056E-4	0.0	0.0	0.0014532560201130633	0.0
19	2.4220933668551056E-4	0.0	0.0	0.0014532560201130633	0.0
20	2.4220933668551056E-4	0.0	0.0	0.0019376746934840845	0.0
21	4.8441867337102113E-4	0.0	0.0	0.003875349386968169	0.0
22	4.8441867337102113E-4	0.0	0.0	0.006055233417137764	0.0
23	4.8441867337102113E-4	0.0	0.0	0.006781861427194296	0.0
24	4.8441867337102113E-4	0.0	0.0	0.007992908110621848	0.0
25	4.8441867337102113E-4	0.0	0.0	0.009688373467420422	0.0
26	4.8441867337102113E-4	0.0	0.0	0.010172792140791443	0.0
27	4.8441867337102113E-4	0.0	0.0	0.02567418968866412	0.0
28	4.8441867337102113E-4	0.0	0.0	0.10415001477476954	0.0
29	4.8441867337102113E-4	0.0	0.0	0.23034107918792054	0.0
30	4.8441867337102113E-4	0.0	0.0	0.39504342813406773	0.0
31	4.8441867337102113E-4	0.0	0.0	0.9383189703196679	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGAA	25	0.0054923147	29.6	22
GGTAAGT	25	0.0054923147	29.6	28
TTCGCTA	25	0.0054923147	29.6	26
GGTATCA	435	0.0	29.34483	1
TACGGCT	55	6.2454274E-7	26.90909	30
TCGTTGG	45	1.3212065E-4	24.666666	2
GTACCTA	55	1.898833E-5	23.545454	1
TATATCT	55	1.898833E-5	23.545454	5
CTATCGC	40	0.0019291028	23.125002	32
TATTGCG	40	0.0019291028	23.125002	11
TATCGCC	40	0.0019291028	23.125002	33
GTATCAA	570	0.0	22.394737	2
TAGAATA	75	3.7287646E-7	22.2	4
GCCTATA	50	2.6980398E-4	22.2	37
TATACAC	135	0.0	21.925926	37
ATTAGAG	70	5.088865E-6	21.142859	3
GGGTACT	70	5.088865E-6	21.142859	1
CTTATAC	500	0.0	21.09	37
GAGTATA	45	0.0038217183	20.555555	1
GGCCCTA	45	0.0038217183	20.555555	11
>>END_MODULE