FastQCFastQC Report
Fri 10 Feb 2017
ERR1631662.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631662.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12151
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA440.362110114393877No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA400.32919101308534277No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC380.31273146243107564No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG360.29627191177680845No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA320.2633528104682742No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT320.2633528104682742No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT260.2139741585054728No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC240.19751460785120567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT240.19751460785120567No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA230.1892848325240721No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA200.16459550654267138No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA200.16459550654267138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT190.15636573121553782No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA190.15636573121553782No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA190.15636573121553782No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA180.14813595588840422No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC180.14813595588840422No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC180.14813595588840422No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA170.1399061805612707No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA170.1399061805612707No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA170.1399061805612707No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT160.1316764052341371No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG150.12344662990700354No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT140.11521685457986997No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA140.11521685457986997No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA140.11521685457986997No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC130.1069870792527364No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT130.1069870792527364No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC130.1069870792527364No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC130.1069870792527364No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC130.1069870792527364No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC130.1069870792527364No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC130.1069870792527364No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT130.1069870792527364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGGG250.00528762429.5999982
GTCTCTT652.3393932E-622.7692337
TGTCTCT903.8932754E-516.44444536
CTGTCTC1308.841036E-411.38461535
Produced by FastQC (version 0.11.5)