##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631662.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12151
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.562258250349764	34.0	31.0	34.0	31.0	34.0
2	32.75195457164019	34.0	31.0	34.0	31.0	34.0
3	32.82626944284421	34.0	31.0	34.0	31.0	34.0
4	36.24952678791869	37.0	37.0	37.0	35.0	37.0
5	36.17405974816887	37.0	35.0	37.0	35.0	37.0
6	36.22549584396346	37.0	36.0	37.0	35.0	37.0
7	36.23249115299152	37.0	37.0	37.0	35.0	37.0
8	36.203357748333474	37.0	37.0	37.0	35.0	37.0
9	38.02905110690478	39.0	38.0	39.0	35.0	39.0
10	37.970701999835406	39.0	38.0	39.0	35.0	39.0
11	38.04180725866184	39.0	38.0	39.0	35.0	39.0
12	37.95753435931199	39.0	38.0	39.0	35.0	39.0
13	38.01785861245988	39.0	38.0	39.0	35.0	39.0
14	39.40893753600527	40.0	39.0	41.0	37.0	41.0
15	39.4249033001399	40.0	39.0	41.0	37.0	41.0
16	39.36424985597893	40.0	39.0	41.0	36.0	41.0
17	39.3267220804872	40.0	39.0	41.0	36.0	41.0
18	39.34400460867418	40.0	39.0	41.0	36.0	41.0
19	39.34902477162373	40.0	39.0	41.0	37.0	41.0
20	39.368940827915395	40.0	39.0	41.0	37.0	41.0
21	39.33421117603489	40.0	39.0	41.0	36.0	41.0
22	39.31857460291334	40.0	39.0	41.0	36.0	41.0
23	39.24508270924204	40.0	39.0	41.0	36.0	41.0
24	39.24039173730557	40.0	39.0	41.0	36.0	41.0
25	39.1498642087071	40.0	39.0	41.0	36.0	41.0
26	39.143938770471564	40.0	39.0	41.0	36.0	41.0
27	39.01259155625051	40.0	39.0	41.0	35.0	41.0
28	38.99004197185417	40.0	39.0	41.0	35.0	41.0
29	38.942556168216605	40.0	38.0	41.0	35.0	41.0
30	38.95712287054563	40.0	38.0	41.0	35.0	41.0
31	38.79779442021233	40.0	38.0	41.0	35.0	41.0
32	38.813677886593695	40.0	38.0	41.0	35.0	41.0
33	38.70471566126245	40.0	38.0	41.0	35.0	41.0
34	38.71302773434285	40.0	38.0	41.0	35.0	41.0
35	38.60118508764711	40.0	38.0	41.0	35.0	41.0
36	38.50547280059254	40.0	38.0	41.0	35.0	41.0
37	38.45090939017365	40.0	38.0	41.0	34.0	41.0
38	38.43749485639042	40.0	38.0	41.0	34.0	41.0
39	38.36927001892848	40.0	38.0	41.0	34.0	41.0
40	38.102954489342444	40.0	37.0	41.0	34.0	41.0
41	38.20516829890544	40.0	37.0	41.0	34.0	41.0
42	38.228870051847586	40.0	37.0	41.0	34.0	41.0
43	37.75598716155049	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	0.0
20	2.0
21	2.0
22	0.0
23	2.0
24	8.0
25	11.0
26	19.0
27	31.0
28	39.0
29	67.0
30	68.0
31	136.0
32	159.0
33	233.0
34	303.0
35	465.0
36	720.0
37	1326.0
38	2912.0
39	5646.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.06238169697967	16.953337173895154	13.044193893506705	27.940087235618467
2	20.66496584643239	19.6609332565221	32.054974899185254	27.619125997860262
3	21.166982141387543	18.920253477080077	30.079828820673193	29.832935560859188
4	15.834087729404986	16.327874249033	34.30170356349272	33.53633445806929
5	16.36902312566867	32.94379063451568	34.23586536087565	16.451320878940003
6	34.01366142704304	33.96428277508024	15.38145008641264	16.640605711464076
7	29.553123199736646	28.409184429265082	20.656736071105257	21.380956299893015
8	27.42984116533619	30.590074890955478	20.66496584643239	21.315118097275946
9	27.791951279730064	12.706773105094232	18.031437741749652	41.46983787342606
10	19.52925685128796	24.030943955230022	28.9029709488931	27.536828244588925
11	38.87745864537898	19.463418648670892	18.418237182124926	23.2408855238252
12	22.434367541766107	24.4424327215867	26.15422598963048	26.968973747016705
13	35.495020985927084	17.274298411653362	21.241050119331742	25.98963048308781
14	23.528927660274874	20.43453213727265	23.874578224014485	32.161961978437986
15	29.69302938029792	24.195539461772693	20.557978767179655	25.553452390749733
16	25.166652950374456	23.644144514854744	22.961073162702657	28.228129372068146
17	26.65624228458563	24.837461937289113	21.603160233725617	26.903135544399635
18	26.31059172084602	21.53732203110855	23.57007653691054	28.582009711134887
19	26.92782487038104	22.40967821578471	23.528927660274874	27.133569253559376
20	30.252654102543	20.51682989054399	23.018681589992593	26.211834416920414
21	27.528598469261787	22.34384001316764	21.79244506624969	28.33511645132088
22	28.70545634104189	23.405481030367874	21.101143938770473	26.78791868981977
23	27.42984116533619	21.67722821166982	23.28203440046087	27.61089622253313
24	27.44630071599045	22.047568101390834	23.70175294214468	26.804378240474037
25	27.660274874495926	22.10517652868077	23.61122541354621	26.623323183277098
26	27.635585548514523	23.39725125504074	22.393218665130444	26.573944531314297
27	25.973170932433543	22.195704057279237	25.397086659534196	26.434038350753024
28	25.627520368693936	22.673031026252982	22.442597317093245	29.25685128795984
29	26.63155295860423	23.043370915973995	23.323183277096536	27.001892848325245
30	27.018352398979506	21.67722821166982	23.891037774668753	27.41338161468192
31	27.73434285244013	22.82939675746852	23.018681589992593	26.417578800098756
32	25.397086659534196	22.52489507036458	23.092749567936796	28.98526870216443
33	25.792115875236604	22.2121636079335	24.3436754176611	27.65204509916879
34	26.39288947411736	22.45082709242038	23.84165912270595	27.314624310756315
35	25.60283104271253	23.53715743560201	25.594601267385404	25.265410254300058
36	26.05546868570488	22.75532877952432	25.00205744383178	26.18714509093902
37	26.886675993745367	22.80470743148712	24.730474858036374	25.578141716731135
38	25.73450744794667	21.80890461690396	26.236523742901817	26.22006419224755
39	25.63575014402107	20.97769730886347	25.528763064768334	27.857789482347133
40	24.886840589251914	21.67722821166982	27.14179902888651	26.29413217019175
41	23.323183277096536	21.413875401201548	27.84955970702	27.41338161468192
42	22.368529339149042	21.413875401201548	28.968809151510165	27.24878610813925
43	21.34803719858448	22.245082709242038	28.43387375524648	27.973006336927003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	1.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	3.0
11	5.0
12	5.0
13	9.0
14	13.0
15	27.0
16	41.0
17	35.5
18	30.0
19	30.0
20	36.5
21	43.0
22	39.5
23	36.0
24	37.5
25	39.0
26	39.0
27	43.0
28	47.0
29	66.0
30	85.0
31	95.5
32	106.0
33	106.0
34	124.5
35	143.0
36	169.0
37	195.0
38	226.5
39	258.0
40	258.0
41	321.5
42	385.0
43	444.0
44	503.0
45	687.5
46	872.0
47	872.0
48	979.0
49	1086.0
50	1232.5
51	1379.0
52	1402.0
53	1425.0
54	1425.0
55	1337.0
56	1249.0
57	1159.5
58	1070.0
59	1007.5
60	945.0
61	945.0
62	847.5
63	750.0
64	601.5
65	453.0
66	396.0
67	339.0
68	339.0
69	297.5
70	256.0
71	209.0
72	162.0
73	121.5
74	81.0
75	81.0
76	63.0
77	45.0
78	43.0
79	41.0
80	35.5
81	30.0
82	30.0
83	22.0
84	14.0
85	11.5
86	9.0
87	7.5
88	6.0
89	6.0
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12151.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.26919595095055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.14742524916943	71.45913916550079
2	5.471345514950166	8.674183194798783
3	1.9206810631229236	4.567525306559131
4	0.8201827242524917	2.600609003374208
5	0.436046511627907	1.7282528186980495
6	0.3633720930232558	1.7282528186980495
7	0.12458471760797342	0.6913011274792198
8	0.10382059800664453	0.6583820261706855
9	0.031146179401993355	0.22220393383260637
>10	0.5813953488372093	7.670150604888487
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	44	0.362110114393877	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	40	0.32919101308534277	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	38	0.31273146243107564	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	36	0.29627191177680845	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	32	0.2633528104682742	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	32	0.2633528104682742	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	26	0.2139741585054728	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	24	0.19751460785120567	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.19751460785120567	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	23	0.1892848325240721	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	20	0.16459550654267138	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	20	0.16459550654267138	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.15636573121553782	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	19	0.15636573121553782	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	19	0.15636573121553782	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	18	0.14813595588840422	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	18	0.14813595588840422	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	18	0.14813595588840422	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	17	0.1399061805612707	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	17	0.1399061805612707	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	17	0.1399061805612707	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.1316764052341371	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	15	0.12344662990700354	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	14	0.11521685457986997	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	14	0.11521685457986997	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	14	0.11521685457986997	No Hit
CCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCAC	13	0.1069870792527364	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	13	0.1069870792527364	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	13	0.1069870792527364	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	13	0.1069870792527364	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	13	0.1069870792527364	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	13	0.1069870792527364	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	13	0.1069870792527364	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	13	0.1069870792527364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.008229775327133568	0.0
11	0.0	0.0	0.0	0.016459550654267137	0.0
12	0.0	0.0	0.0	0.016459550654267137	0.0
13	0.0	0.0	0.0	0.016459550654267137	0.0
14	0.0	0.0	0.0	0.016459550654267137	0.0
15	0.0	0.0	0.0	0.016459550654267137	0.0
16	0.0	0.0	0.0	0.016459550654267137	0.0
17	0.0	0.0	0.0	0.016459550654267137	0.0
18	0.0	0.0	0.0	0.016459550654267137	0.0
19	0.0	0.0	0.0	0.016459550654267137	0.0
20	0.0	0.0	0.0	0.016459550654267137	0.0
21	0.0	0.0	0.0	0.016459550654267137	0.0
22	0.0	0.0	0.0	0.016459550654267137	0.0
23	0.0	0.0	0.0	0.016459550654267137	0.0
24	0.0	0.0	0.0	0.02468932598140071	0.0
25	0.0	0.0	0.0	0.02468932598140071	0.0
26	0.0	0.0	0.0	0.02468932598140071	0.0
27	0.0	0.0	0.0	0.041148876635667846	0.0
28	0.0	0.0	0.0	0.09052752859846926	0.0
29	0.0	0.0	0.0	0.25512303514114065	0.0
30	0.0	0.0	0.0	0.4032589910295449	0.0
31	0.0	0.0	0.0	0.8147477573862234	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTGGG	25	0.005287624	29.599998	2
GTCTCTT	65	2.3393932E-6	22.76923	37
TGTCTCT	90	3.8932754E-5	16.444445	36
CTGTCTC	130	8.841036E-4	11.384615	35
>>END_MODULE