FastQCFastQC Report
Fri 10 Feb 2017
ERR1631661.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631661.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420506
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16090.38263425492145176No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT11640.2768093677616966No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG7680.18263710862627408No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT6710.15956966131279934No Hit
GTGTGGGGCTGCCTGCAGGCTGCGTCTAGTTGCAGTAGTTCTC6420.15267320799227596No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT6360.1512463555811332No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT6240.14839265075884767No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG6130.1457767546717526No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6030.143398667319848No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5770.13721564020489602No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5460.12984356941399172No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5350.12722767332689666No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG5240.12461177723980157No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT5160.12270930735827788No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG5130.12199588115270651No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGTGGG5110.12152026368232559No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG5050.12009341127118282No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC5020.11937998506561143No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4870.11581285403775453No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4860.11557504530256406No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4500.10701393083570745No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4400.10463584348380285No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG4380.10416022601342192No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG4300.10225775613189823No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4290.10201994739670776No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTAAG303.5956313E-430.8333341
ACCGGGA250.00549242929.68
AACTGAG250.00549242929.67
GTAGTAT250.00549242929.61
GGTATCA4150.028.97591
GACAGGC4250.025.6823547
ACAGGCT4300.024.9534878
CAGGACA4650.024.6666664
TGCATCA4400.024.38636414
GGACAGG4750.024.1473676
TGAGTCG551.8989409E-523.5454544
CCAGGAC4700.023.2234023
CATCAGA4700.023.22340216
ATCAGAA4950.022.05050517
TCCAGGA5200.021.7019232
ATCAAGC4900.021.14285930
GCTGCAT5100.021.03921512
TCAGAAG5200.020.99038518
AGAAGAG4950.020.92929320
CCATCAA5100.020.6764728
Produced by FastQC (version 0.11.5)