ERR1631660.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31560
Sequences flagged as poor quality	0
Sequence length	43
%GC	54

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	73	0.23130544993662863	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	67	0.2122940430925222	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	64	0.20278833967046894	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	63	0.19961977186311788	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	63	0.19961977186311788	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	61	0.1932826362484157	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	57	0.1806083650190114	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	55	0.17427122940430925	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	50	0.15842839036755385	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	49	0.1552598225602028	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	48	0.1520912547528517	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	47	0.14892268694550065	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	45	0.14258555133079848	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	43	0.13624841571609633	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	43	0.13624841571609633	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	41	0.12991128010139416	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	40	0.12674271229404308	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	40	0.12674271229404308	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	39	0.123574144486692	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	38	0.12040557667934094	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	38	0.12040557667934094	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	38	0.12040557667934094	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	38	0.12040557667934094	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	37	0.11723700887198987	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	36	0.11406844106463879	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	35	0.1108998732572877	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	35	0.1108998732572877	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	35	0.1108998732572877	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	35	0.1108998732572877	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	34	0.10773130544993663	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	33	0.10456273764258554	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	33	0.10456273764258554	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	33	0.10456273764258554	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTCG	20	0.0018149407	37.0	35
ACCACGT	25	0.005416704	29.6	5
TACCACG	25	0.005416704	29.6	4
GGTATCA	35	8.67888E-4	26.42857	1
GTATCAA	50	0.006888013	18.5	2
CTCTTAT	90	4.229785E-5	16.444445	37
GTCTCTT	205	7.6397555E-11	14.439024	37
TCAACGC	80	0.0061214357	13.875	3
ATCAACG	80	0.0061214357	13.875	2
AACGCAG	85	0.009142339	13.058824	5
ACGCAGA	85	0.009142339	13.058824	6
CGCAGAG	85	0.009142339	13.058824	7
TATCAAC	85	0.009142339	13.058824	1
GCCTGTC	85	0.009142339	13.058824	36
GCAGAGT	105	0.0025466608	12.333334	8
TCTCTTA	125	6.8828394E-4	11.84	36
TGTCTCT	280	1.0599251E-8	10.571428	36
CCTGTCT	215	5.707643E-6	10.325582	37
CTGTCTC	325	1.0685471E-7	9.107692	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content