##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631660.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31560
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.55532319391635	34.0	31.0	34.0	31.0	34.0
2	32.72610899873257	34.0	31.0	34.0	31.0	34.0
3	32.81530418250951	34.0	31.0	34.0	31.0	34.0
4	36.25769961977186	37.0	37.0	37.0	35.0	37.0
5	36.156115335868186	37.0	35.0	37.0	35.0	37.0
6	36.222148288973386	37.0	36.0	37.0	35.0	37.0
7	36.20722433460076	37.0	36.0	37.0	35.0	37.0
8	36.19011406844106	37.0	36.0	37.0	35.0	37.0
9	38.017807351077316	39.0	38.0	39.0	35.0	39.0
10	37.94600760456274	39.0	38.0	39.0	35.0	39.0
11	38.02661596958175	39.0	38.0	39.0	35.0	39.0
12	37.94594423320659	39.0	38.0	39.0	35.0	39.0
13	38.029911280101395	39.0	38.0	39.0	35.0	39.0
14	39.37043726235741	40.0	39.0	41.0	37.0	41.0
15	39.38837135614702	40.0	39.0	41.0	37.0	41.0
16	39.32820025348543	40.0	39.0	41.0	36.0	41.0
17	39.3074144486692	40.0	39.0	41.0	36.0	41.0
18	39.316001267427126	40.0	39.0	41.0	36.0	41.0
19	39.33770595690748	40.0	39.0	41.0	36.0	41.0
20	39.314860583016475	40.0	39.0	41.0	36.0	41.0
21	39.291825095057035	40.0	39.0	41.0	36.0	41.0
22	39.292458808618505	40.0	39.0	41.0	36.0	41.0
23	39.20541825095057	40.0	39.0	41.0	36.0	41.0
24	39.202756653992395	40.0	39.0	41.0	36.0	41.0
25	39.16669835234474	40.0	39.0	41.0	36.0	41.0
26	39.09119138149556	40.0	39.0	41.0	36.0	41.0
27	38.95443599493029	40.0	39.0	41.0	35.0	41.0
28	38.958174904942965	40.0	38.0	41.0	35.0	41.0
29	38.94508871989861	40.0	38.0	41.0	35.0	41.0
30	38.86520912547528	40.0	38.0	41.0	35.0	41.0
31	38.814512040557666	40.0	38.0	41.0	35.0	41.0
32	38.79543726235742	40.0	38.0	41.0	35.0	41.0
33	38.72639416983523	40.0	38.0	41.0	35.0	41.0
34	38.68475918884664	40.0	38.0	41.0	35.0	41.0
35	38.63339670468948	40.0	38.0	41.0	35.0	41.0
36	38.591349809885934	40.0	38.0	41.0	35.0	41.0
37	38.53368187579214	40.0	38.0	41.0	35.0	41.0
38	38.50354879594423	40.0	38.0	41.0	34.0	41.0
39	38.41530418250951	40.0	38.0	41.0	34.0	41.0
40	38.09775031685678	40.0	37.0	41.0	34.0	41.0
41	38.18121039290241	40.0	37.0	41.0	34.0	41.0
42	38.172718631178704	40.0	37.0	41.0	34.0	41.0
43	37.70738276299113	40.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	1.0
20	0.0
21	2.0
22	4.0
23	10.0
24	14.0
25	34.0
26	44.0
27	70.0
28	94.0
29	154.0
30	226.0
31	307.0
32	430.0
33	579.0
34	831.0
35	1349.0
36	1943.0
37	3330.0
38	7623.0
39	14514.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.041825095057035	16.137515842839036	12.702788339670468	28.11787072243346
2	21.26742712294043	19.53422053231939	31.359315589353614	27.839036755386566
3	21.644486692015207	18.87832699619772	28.8212927756654	30.655893536121674
4	16.029784537389098	16.270595690747783	33.346007604562736	34.353612167300376
5	17.506337135614704	32.40177439797212	32.87705956907478	17.214828897338403
6	35.17427122940431	32.544359949302915	15.243979721166031	17.03738910012674
7	30.602027883396705	26.932826362484157	19.892268694550065	22.572877059569073
8	27.664765525982254	30.186945500633716	20.38339670468948	21.76489226869455
9	28.041825095057032	12.5	17.864385297845374	41.59378960709759
10	19.898605830164765	23.298479087452474	29.039923954372625	27.76299112801014
11	39.49619771863118	19.074778200253487	18.865652724968314	22.563371356147023
12	22.924588086185043	23.26362484157161	26.321292775665402	27.490494296577943
13	35.79214195183777	17.069074778200253	20.117237008871992	27.021546261089984
14	24.08745247148289	20.53865652724968	23.130544993662863	32.243346007604565
15	30.095057034220535	23.485424588086186	20.05069708491762	26.368821292775664
16	25.430925221799743	23.425221799746517	22.325728770595692	28.818124207858048
17	27.268694550063373	23.624841571609632	21.514575411913814	27.591888466413184
18	27.230671736375157	20.4404309252218	23.181242078580482	29.147655259822557
19	28.330164765525982	22.03422053231939	22.73764258555133	26.8979721166033
20	30.513307984790877	20.89987325728771	22.097591888466415	26.489226869455006
21	28.013307984790874	21.70468948035488	21.365652724968314	28.916349809885933
22	29.131812420785806	22.75982256020279	20.367553865652724	27.74081115335868
23	28.656527249683144	21.346641318124206	21.790240811153357	28.20659062103929
24	28.083016476552597	20.8871989860583	22.658428390367554	28.371356147021547
25	29.058935361216733	21.40684410646388	22.40811153358682	27.126108998732573
26	28.40621039290241	22.41444866920152	22.335234474017742	26.844106463878326
27	27.214828897338407	21.29911280101394	23.992395437262356	27.4936628643853
28	26.818757921419518	22.55069708491762	21.6191381495564	29.011406844106464
29	27.230671736375157	23.32065906210393	22.313054499366284	27.135614702154626
30	27.21166032953105	21.730038022813687	22.832699619771862	28.225602027883394
31	28.422053231939167	21.94866920152091	22.00253485424588	27.626742712294046
32	26.26425855513308	21.74271229404309	22.854879594423323	29.138149556400506
33	26.229404309252217	21.305449936628644	24.59125475285171	27.873891001267424
34	26.88529784537389	21.666666666666668	22.728136882129277	28.719898605830164
35	26.26742712294043	23.342839036755386	24.255386565272495	26.134347275031683
36	26.685678073510772	22.224334600760457	24.03358681875792	27.056400506970853
37	26.73003802281369	21.682509505703422	24.61660329531052	26.97084917617237
38	25.430925221799743	21.264258555133082	25.019011406844104	28.285804816223063
39	25.288339670468947	20.101394169835235	26.327629911280106	28.282636248415717
40	25.484790874524716	21.181875792141952	26.783903675538657	26.549429657794676
41	23.811787072243344	20.399239543726235	27.88656527249683	27.90240811153359
42	21.730038022813687	20.453105196451205	29.47401774397972	28.342839036755386
43	21.80291508238276	21.210392902408113	29.08428390367554	27.90240811153359
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	1.0
4	2.0
5	2.0
6	2.0
7	2.0
8	2.5
9	3.0
10	5.0
11	7.0
12	7.0
13	10.0
14	13.0
15	29.5
16	46.0
17	47.5
18	49.0
19	49.0
20	50.5
21	52.0
22	47.0
23	42.0
24	50.0
25	58.0
26	58.0
27	73.0
28	88.0
29	102.5
30	117.0
31	155.0
32	193.0
33	193.0
34	250.0
35	307.0
36	334.0
37	361.0
38	471.5
39	582.0
40	582.0
41	744.0
42	906.0
43	1076.5
44	1247.0
45	1810.0
46	2373.0
47	2373.0
48	2622.0
49	2871.0
50	3194.5
51	3518.0
52	3524.5
53	3531.0
54	3531.0
55	3295.5
56	3060.0
57	2964.0
58	2868.0
59	2667.0
60	2466.0
61	2466.0
62	2292.5
63	2119.0
64	1789.0
65	1459.0
66	1257.0
67	1055.0
68	1055.0
69	911.0
70	767.0
71	665.0
72	563.0
73	446.5
74	330.0
75	330.0
76	263.5
77	197.0
78	164.0
79	131.0
80	105.5
81	80.0
82	80.0
83	67.0
84	54.0
85	43.0
86	32.0
87	20.5
88	9.0
89	9.0
90	5.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	31560.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.98436839881707	68.2382762991128
2	4.583861427967892	6.8757921419518375
3	1.6223067173637515	3.650190114068441
4	0.6928601605407689	2.0785804816223066
5	0.4985213350232362	1.8694550063371358
6	0.2619349387410224	1.1787072243346008
7	0.2492606675116181	1.308618504435995
8	0.17743979721166034	1.064638783269962
9	0.08449514152936206	0.5703422053231939
>10	0.8111533586818758	11.571609632446133
>50	0.03379805661174483	1.5937896070975919
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	73	0.23130544993662863	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	67	0.2122940430925222	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	64	0.20278833967046894	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	63	0.19961977186311788	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	63	0.19961977186311788	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	61	0.1932826362484157	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	57	0.1806083650190114	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	55	0.17427122940430925	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	50	0.15842839036755385	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	49	0.1552598225602028	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	48	0.1520912547528517	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	47	0.14892268694550065	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	45	0.14258555133079848	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	43	0.13624841571609633	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	43	0.13624841571609633	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	41	0.12991128010139416	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	40	0.12674271229404308	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	40	0.12674271229404308	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	39	0.123574144486692	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	38	0.12040557667934094	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	38	0.12040557667934094	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	38	0.12040557667934094	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	38	0.12040557667934094	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	37	0.11723700887198987	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	36	0.11406844106463879	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	35	0.1108998732572877	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	35	0.1108998732572877	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	35	0.1108998732572877	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	35	0.1108998732572877	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	34	0.10773130544993663	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	33	0.10456273764258554	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	33	0.10456273764258554	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	33	0.10456273764258554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0031685678073510772	0.0
17	0.0	0.0	0.0	0.0031685678073510772	0.0
18	0.0	0.0	0.0	0.0031685678073510772	0.0
19	0.0	0.0	0.0	0.0031685678073510772	0.0
20	0.0	0.0	0.0	0.0031685678073510772	0.0
21	0.0	0.0	0.0	0.0063371356147021544	0.0
22	0.0	0.0	0.0	0.012674271229404309	0.0
23	0.0	0.0	0.0	0.012674271229404309	0.0
24	0.0	0.0	0.0	0.012674271229404309	0.0
25	0.0	0.0	0.0	0.015842839036755388	0.0
26	0.0	0.0	0.0	0.015842839036755388	0.0
27	0.0	0.0	0.0	0.041191381495564006	0.0
28	0.0	0.0	0.0	0.12991128010139416	0.0
29	0.0	0.0	0.0	0.3231939163498099	0.0
30	0.0	0.0	0.0	0.5038022813688213	0.0
31	0.0	0.0	0.0	0.9283903675538656	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTCG	20	0.0018149407	37.0	35
ACCACGT	25	0.005416704	29.6	5
TACCACG	25	0.005416704	29.6	4
GGTATCA	35	8.67888E-4	26.42857	1
GTATCAA	50	0.006888013	18.5	2
CTCTTAT	90	4.229785E-5	16.444445	37
GTCTCTT	205	7.6397555E-11	14.439024	37
TCAACGC	80	0.0061214357	13.875	3
ATCAACG	80	0.0061214357	13.875	2
AACGCAG	85	0.009142339	13.058824	5
ACGCAGA	85	0.009142339	13.058824	6
CGCAGAG	85	0.009142339	13.058824	7
TATCAAC	85	0.009142339	13.058824	1
GCCTGTC	85	0.009142339	13.058824	36
GCAGAGT	105	0.0025466608	12.333334	8
TCTCTTA	125	6.8828394E-4	11.84	36
TGTCTCT	280	1.0599251E-8	10.571428	36
CCTGTCT	215	5.707643E-6	10.325582	37
CTGTCTC	325	1.0685471E-7	9.107692	35
>>END_MODULE