FastQCFastQC Report
Fri 10 Feb 2017
ERR1631659.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631659.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078558
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC27600.255897225740294No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT20640.19136662098839374No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG12560.11645178098906132No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT11330.10504766549411344No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT11130.10319333777135768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7250.031.8965511
TATCCGG652.6825928E-622.7692310
AATCGTA450.003825657820.5555556
TGTCCGA656.9016285E-519.92307712
GTATCAA11500.019.7869572
GACAGGC8100.019.6419757
GTATAGA951.6758713E-719.4736841
ACAGGCT8350.018.6107798
CTTATAC11700.018.1837637
GGACAGG10000.017.766
CTATCCG953.6059682E-617.5263169
TGCATCA8800.017.23863614
TATTAGA2150.017.2093032
TAGAACA1401.8681021E-917.1785724
ATTAGAG2100.016.7380943
CAGGACA11300.016.6991164
GCATCAG9250.016.615
CCAGGAC11050.016.574663
TCTTATA20700.016.35507237
ATCAGAA9300.016.31182717
Produced by FastQC (version 0.11.5)