##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631658.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3813
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47469184369263	33.0	31.0	34.0	31.0	34.0
2	32.60713349068975	34.0	31.0	34.0	31.0	34.0
3	32.6464725937582	34.0	31.0	34.0	31.0	34.0
4	36.138473642800946	37.0	35.0	37.0	35.0	37.0
5	36.034880671387356	37.0	35.0	37.0	35.0	37.0
6	36.09415158667716	37.0	35.0	37.0	35.0	37.0
7	36.09336480461579	37.0	35.0	37.0	35.0	37.0
8	36.0422239706268	37.0	35.0	37.0	35.0	37.0
9	37.936532913716235	39.0	38.0	39.0	35.0	39.0
10	37.782848151062154	39.0	38.0	39.0	35.0	39.0
11	37.93207448203514	39.0	38.0	39.0	35.0	39.0
12	37.814319433516914	39.0	38.0	39.0	35.0	39.0
13	37.90375032782586	39.0	38.0	39.0	35.0	39.0
14	39.27143981117231	40.0	39.0	41.0	36.0	41.0
15	39.28350380277996	40.0	39.0	41.0	36.0	41.0
16	39.13899816417519	40.0	39.0	41.0	36.0	41.0
17	39.079202727511145	40.0	39.0	41.0	36.0	41.0
18	39.17309205350118	40.0	39.0	41.0	36.0	41.0
19	39.16024127983216	40.0	39.0	41.0	36.0	41.0
20	39.0923157618673	40.0	39.0	41.0	36.0	41.0
21	39.08838185156045	40.0	39.0	41.0	36.0	41.0
22	39.09572515079989	40.0	39.0	41.0	36.0	41.0
23	38.95069499082088	40.0	39.0	41.0	35.0	41.0
24	38.91948596905324	40.0	39.0	41.0	35.0	41.0
25	38.99003409388933	40.0	39.0	41.0	35.0	41.0
26	38.93994230264883	40.0	38.0	41.0	35.0	41.0
27	38.80540257015473	40.0	38.0	41.0	35.0	41.0
28	38.76212955677944	40.0	38.0	41.0	35.0	41.0
29	38.80828743771308	40.0	38.0	41.0	35.0	41.0
30	38.787831104117494	40.0	38.0	41.0	35.0	41.0
31	38.66745344872803	40.0	38.0	41.0	35.0	41.0
32	38.600839234198794	40.0	38.0	41.0	35.0	41.0
33	38.599003409388935	40.0	38.0	41.0	35.0	41.0
34	38.58405455022292	40.0	38.0	41.0	35.0	41.0
35	38.43036978756884	40.0	38.0	41.0	34.0	41.0
36	38.3443482821925	40.0	38.0	41.0	34.0	41.0
37	38.3147128245476	40.0	38.0	41.0	34.0	41.0
38	38.29058484133228	40.0	38.0	41.0	34.0	41.0
39	38.26173616574876	40.0	37.0	41.0	34.0	41.0
40	37.98741148701809	40.0	37.0	41.0	33.0	41.0
41	38.06215578284815	40.0	37.0	41.0	33.0	41.0
42	38.084185680566485	40.0	37.0	41.0	34.0	41.0
43	37.56307369525308	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	3.0
23	3.0
24	2.0
25	3.0
26	7.0
27	14.0
28	19.0
29	32.0
30	29.0
31	43.0
32	62.0
33	83.0
34	95.0
35	185.0
36	242.0
37	379.0
38	902.0
39	1709.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.48623131392604	16.207710464201416	13.191712562286915	28.114345659585627
2	22.554419092578023	17.414109624967217	31.497508523472334	28.53396275898243
3	21.21688958825072	20.06294256490952	28.822449514817727	29.897718332022027
4	15.630736952530816	16.574875426173616	33.59559402045633	34.19879360083923
5	16.679779701022817	33.30710726462103	32.28429058484133	17.728822449514816
6	33.96275898242853	33.857854707579335	14.896407028586415	17.282979281405716
7	31.051665355363234	28.009441384736427	19.013899816417517	21.92499344348282
8	27.511146079202724	31.13034356150013	21.03330710726462	20.32520325203252
9	26.986624704956725	12.326252294781012	17.282979281405716	43.40414371885654
10	19.40729084710202	24.600052452137426	28.95357985837923	27.039076842381327
11	39.36532913716234	19.302386572252818	18.90899554156832	22.423288749016525
12	22.869131917125625	23.21007081038552	26.330972987149227	27.589824285339624
13	33.857854707579335	19.32861264096512	20.744820351429322	26.068712300026224
14	24.836087070548125	19.51219512195122	23.86572252819302	31.785995279307635
15	30.605822187254127	23.577235772357724	19.56464725937582	26.252294781012324
16	24.180435352740624	23.970626803042226	22.370836611591923	29.47810123262523
17	25.045895620246526	24.783634933123526	22.606871230002625	27.563598216627327
18	26.671911880409127	20.875950694990824	22.921584054550223	29.530553370049827
19	29.50432730133753	21.08575924468922	22.659323367427223	26.750590086546026
20	28.79622344610543	21.00708103855232	22.423288749016525	27.773406766325724
21	28.24547600314713	22.738001573564123	21.45292420666142	27.563598216627327
22	28.95357985837923	23.86572252819302	20.666142145292422	26.514555468135327
23	27.852084972462627	21.374246000524522	23.105166535536323	27.668502491476527
24	27.065302911093625	22.239706268030424	22.79045371098872	27.90453710988723
25	27.668502491476527	21.92499344348282	23.524783634933126	26.881720430107524
26	26.72436401783373	23.68214004720692	23.183844741673223	26.409651193286127
27	27.327563598216624	22.449514817728822	22.947810123262524	27.27511146079203
28	26.19984264358773	22.50196695515342	23.026488329399424	28.27170207185943
29	27.117755048518227	23.000262260687123	22.16102806189352	27.72095462890113
30	25.517964857067927	23.498557566220825	23.787044322056126	27.196433254655126
31	27.484920010490427	22.213480199318123	24.285339627589824	26.01626016260163
32	25.911355887752425	22.685549436139524	24.180435352740624	27.222659323367427
33	25.93758195646473	22.97403619197482	25.124573826383422	25.963808025177027
34	27.039076842381327	20.90217676370312	24.678730658274326	27.380015735641226
35	25.963808025177027	23.760818253343825	24.967217414109623	25.308156307369522
36	25.517964857067927	22.554419092578023	23.970626803042226	27.956989247311824
37	25.124573826383422	21.767637031209023	25.098347757671124	28.009441384736427
38	25.858903750327826	20.79727248885392	25.49173878835563	27.852084972462627
39	24.626278520849727	19.45974298452662	28.08811959087333	27.825858903750326
40	24.180435352740624	19.69577760293732	28.193023865722527	27.930763178599527
41	22.869131917125625	19.82690794649882	30.13375295043273	27.17020718594283
42	21.24311565696302	19.82690794649882	31.051665355363234	27.878311041174932
43	21.71518489378442	20.32520325203252	30.42223970626803	27.53737214791503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	1.0
11	2.0
12	2.0
13	3.5
14	5.0
15	7.0
16	9.0
17	10.5
18	12.0
19	12.0
20	11.0
21	10.0
22	8.5
23	7.0
24	8.5
25	10.0
26	10.0
27	11.5
28	13.0
29	17.0
30	21.0
31	28.5
32	36.0
33	36.0
34	45.0
35	54.0
36	62.5
37	71.0
38	86.5
39	102.0
40	102.0
41	124.0
42	146.0
43	168.0
44	190.0
45	229.0
46	268.0
47	268.0
48	303.5
49	339.0
50	357.5
51	376.0
52	388.0
53	400.0
54	400.0
55	379.0
56	358.0
57	324.5
58	291.0
59	273.5
60	256.0
61	256.0
62	247.5
63	239.0
64	211.5
65	184.0
66	158.5
67	133.0
68	133.0
69	107.5
70	82.0
71	78.0
72	74.0
73	67.5
74	61.0
75	61.0
76	48.5
77	36.0
78	25.0
79	14.0
80	9.5
81	5.0
82	5.0
83	5.0
84	5.0
85	2.5
86	0.0
87	1.0
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3813.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.1846315237346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.45234708392604	87.0705481248361
2	3.783783783783784	6.976134277471807
3	1.1948790896159318	3.304484657749803
4	0.25604551920341395	0.9441384736428009
5	0.1991465149359886	0.917912404930501
6	0.05689900426742533	0.3147128245476003
7	0.0	0.0
8	0.028449502133712664	0.2098085496984002
9	0.0	0.0
>10	0.028449502133712664	0.26226068712300027
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	10	0.26226068712300027	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.2098085496984002	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	6	0.15735641227380015	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	6	0.15735641227380015	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	5	0.13113034356150013	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	5	0.13113034356150013	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	5	0.13113034356150013	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	5	0.13113034356150013	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.13113034356150013	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	5	0.13113034356150013	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.13113034356150013	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	4	0.1049042748492001	No Hit
GACGTTACCCGCAGAAGAAGCACCGGCTAACTCCGTGCCAGCA	4	0.1049042748492001	No Hit
ACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGA	4	0.1049042748492001	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	4	0.1049042748492001	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	4	0.1049042748492001	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	4	0.1049042748492001	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	4	0.1049042748492001	No Hit
CCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTC	4	0.1049042748492001	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4	0.1049042748492001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.1835824809861002	0.0
29	0.0	0.0	0.0	0.3147128245476003	0.0
30	0.0	0.0	0.0	0.6556517178075006	0.0
31	0.0	0.0	0.0	1.4424337791765014	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE