FastQCFastQC Report
Fri 10 Feb 2017
ERR1631657.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631657.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252362
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11090.43944809440407034No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10200.40418129512367157No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA10000.3962561716898741No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9810.38872730442776643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.3855572550542475No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9310.36891449584327274No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8680.3439503570268107No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG8590.34038405148160183No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA8520.3376102582797727No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT7950.3150236564934499No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7910.3134386318066904No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC7480.2963996164240258No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7310.289663261505298No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6680.2646991226888359No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6400.2536039498815194No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA6340.2512264128513802No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6240.24726385113448143No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6070.24052749621575356No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6040.23933872770068393No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5990.2373574468422346No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5870.23260237278195606No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5810.23022483575181685No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5790.22943232340843708No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5730.22705478637829782No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5520.21873340677281047No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5410.2143745888842219No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5120.20288315990521552No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5050.20010936670338642No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4920.19495803647141804No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4910.19456178029972818No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4910.19456178029972818No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4620.18307035132072183No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4610.18267409514903196No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4550.1802965581188927No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4510.1787115334321332No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4480.17752276491706356No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT4350.17237143468509522No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4230.16761636062481675No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT4230.16761636062481675No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT4190.16603133593805725No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA4010.1588987248476395No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC3870.15335113844398127No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3860.15295488227229137No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3780.1497848328987724No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT3770.1493885767270825No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3740.1481998082120129No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3730.14780355204032303No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT3680.14582227118187366No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3670.1454260150101838No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3600.14265222180835466No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3590.1422559656366648No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA3530.13987842860652555No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3480.13789714774807618No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC3420.13551961071793692No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3420.13551961071793692No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT3350.1327458175161078No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3290.13036828048596857No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3240.12838699962751918No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3220.12759448728413944No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3210.12719823111244957No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3140.12442443791062045No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3090.12244315705217108No Hit
CAGTAATTCCGATTAACGCTTGCACCCTCCGTATTACCGCGGC3070.12165064470879133No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3040.12046187619372173No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT3040.12046187619372173No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT3000.11887685150696221No Hit
CTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTT3000.11887685150696221No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCC2990.11848059533527235No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2970.11768808299189261No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2960.11729182682020273No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2950.11689557064851286No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA2940.11649931447682298No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2910.11531054596175334No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2890.1145180336183736No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT2870.11372552127499386No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2870.11372552127499386No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2850.1129330089316141No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2820.11174424041654447No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2810.11134798424485462No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2800.11095172807316474No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2790.11055547190147487No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2790.11055547190147487No Hit
AGCCTGATGCAGCCATGCCGCGTGTATGAAGAAGGCCTTCGGG2770.10976295955809512No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2750.10897044721471538No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2750.10897044721471538No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2690.10659291018457612No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2690.10659291018457612No Hit
TTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACATTG2670.10580039784119637No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2630.10421537315443688No Hit
GGCACGGAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATG2570.10183783612429764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6750.030.9703711
ACAGGTT303.5915922E-430.83333236
GAACAGT250.005488318429.61
ACTGTGC358.850148E-426.428578
ACACGAA358.850148E-426.4285717
ACTCGAC358.850148E-426.4285736
GTGCAAA358.850148E-426.4285711
AAACACG358.850148E-426.4285715
CACTGTG358.850148E-426.428577
TAGTCCG358.850148E-426.4285715
TTTTAAC358.850148E-426.428572
ATTGTAG358.850148E-426.428571
CTGTGCA358.850148E-426.428579
CGTCGTA358.850148E-426.4285710
TCGTAGT358.850148E-426.4285712
GACCTGC358.850148E-426.428576
TTGGAGT358.850148E-426.4285724
CAAACAC358.850148E-426.4285714
TTTAACC358.850148E-426.428573
GGATTGG451.319218E-424.66666821
Produced by FastQC (version 0.11.5)