FastQCFastQC Report
Fri 10 Feb 2017
ERR1631656.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631656.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26608
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA770.2893866506313891No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA710.26683704149128085No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA690.25932050511124477No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA630.2367708959711365No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC620.23301262778111848No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC540.20294648226097414No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA530.1991882140709561No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG510.19167167769092003No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT500.187913409500902No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT490.18415514131088395No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA480.1803968731208659No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA470.17663860493084788No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA450.1691220685508118No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT390.14657245941070354No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA390.14657245941070354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380.1428141912206855No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT370.13905592303066747No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA340.12778111846061335No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA340.12778111846061335No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG330.12402285027059531No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC320.12026458208057728No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG320.12026458208057728No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC310.11650631389055924No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA310.11650631389055924No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC300.1127480457005412No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT300.1127480457005412No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC290.10898977751052316No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC290.10898977751052316No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA290.10898977751052316No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA290.10898977751052316No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC280.10523150932050512No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC280.10523150932050512No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA280.10523150932050512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACA250.005401552729.637
GCTGCTG250.005401552729.616
GGTATCA603.99923E-827.7500021
TCTTATA652.5232057E-622.7692337
GTATCAA1005.4565207E-616.6500022
CTCTTAT1051.5618591E-414.09523836
TATCAAC1359.273509E-512.3333341
ATCAACG1359.273509E-512.3333342
AACGCAG1401.3024594E-411.8928585
TCAACGC1401.3024594E-411.8928583
CAACGCA1401.3024594E-411.8928584
TATCACC1100.00358310911.7727276
CTGTCTC3255.456968E-1211.38461537
ACGCAGA1502.4748954E-411.0999996
CAGAGTA1502.4748954E-411.0999999
AGAGTAC1502.4748954E-411.09999910
GAGTACG1502.4748954E-411.09999911
GCAGAGT1502.4748954E-411.0999998
CGCAGAG1502.4748954E-411.0999997
AGTACGG1553.353852E-410.74193612
Produced by FastQC (version 0.11.5)