##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631656.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26608
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52833734215274	34.0	31.0	34.0	31.0	34.0
2	32.69219783523752	34.0	31.0	34.0	31.0	34.0
3	32.7916416115454	34.0	31.0	34.0	31.0	34.0
4	36.24195730607336	37.0	37.0	37.0	35.0	37.0
5	36.148376428141916	37.0	35.0	37.0	35.0	37.0
6	36.180359290438965	37.0	36.0	37.0	35.0	37.0
7	36.192423331328925	37.0	36.0	37.0	35.0	37.0
8	36.17295550210463	37.0	36.0	37.0	35.0	37.0
9	38.00259320505111	39.0	38.0	39.0	35.0	39.0
10	37.93419272399279	39.0	38.0	39.0	35.0	39.0
11	38.00484816596512	39.0	38.0	39.0	35.0	39.0
12	37.943550811785926	39.0	38.0	39.0	35.0	39.0
13	37.99605381840048	39.0	38.0	39.0	35.0	39.0
14	39.34260372820204	40.0	39.0	41.0	36.0	41.0
15	39.340348767288035	40.0	39.0	41.0	36.0	41.0
16	39.29799308478653	40.0	39.0	41.0	36.0	41.0
17	39.27585688514733	40.0	39.0	41.0	36.0	41.0
18	39.304645219482865	40.0	39.0	41.0	36.0	41.0
19	39.31761124473842	40.0	39.0	41.0	36.0	41.0
20	39.31013229104029	40.0	39.0	41.0	36.0	41.0
21	39.270369813589895	40.0	39.0	41.0	36.0	41.0
22	39.241994888755265	40.0	39.0	41.0	36.0	41.0
23	39.18107336139507	40.0	39.0	41.0	36.0	41.0
24	39.214371617558626	40.0	39.0	41.0	36.0	41.0
25	39.14225045099218	40.0	39.0	41.0	36.0	41.0
26	39.102826217678896	40.0	39.0	41.0	36.0	41.0
27	38.95223241130487	40.0	39.0	41.0	35.0	41.0
28	38.952420324714375	40.0	39.0	41.0	35.0	41.0
29	38.945768190018036	40.0	38.0	41.0	35.0	41.0
30	38.89890258568852	40.0	38.0	41.0	35.0	41.0
31	38.80280366806976	40.0	38.0	41.0	35.0	41.0
32	38.76488274203247	40.0	38.0	41.0	35.0	41.0
33	38.70373571858088	40.0	38.0	41.0	35.0	41.0
34	38.67310583283223	40.0	38.0	41.0	35.0	41.0
35	38.61846061334937	40.0	38.0	41.0	35.0	41.0
36	38.59098767288034	40.0	38.0	41.0	35.0	41.0
37	38.53100571256765	40.0	38.0	41.0	34.0	41.0
38	38.50646422128683	40.0	38.0	41.0	35.0	41.0
39	38.46501052315093	40.0	38.0	41.0	34.0	41.0
40	38.12082832230908	40.0	37.0	41.0	34.0	41.0
41	38.231885147324114	40.0	37.0	41.0	34.0	41.0
42	38.21662657847264	40.0	37.0	41.0	34.0	41.0
43	37.68915363800361	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	5.0
23	9.0
24	17.0
25	24.0
26	36.0
27	60.0
28	93.0
29	145.0
30	185.0
31	269.0
32	338.0
33	475.0
34	733.0
35	1070.0
36	1694.0
37	2813.0
38	6503.0
39	12138.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.72053517739026	17.19783523752255	13.326819001803969	27.754810583283223
2	20.535177390258568	20.332230907997594	32.542844257366205	26.589747444377632
3	20.332230907997594	19.625676488274205	30.148827420324714	29.89326518340349
4	15.28111846061335	16.773150932050513	34.48962717979555	33.45610342754059
5	16.1041791942273	33.74549007817198	33.805622369212266	16.344708358388456
6	32.88108839446783	34.78653036680698	16.013980757666868	16.31840048105833
7	29.630186410102226	28.34861695730607	20.520144317498495	21.501052315093204
8	26.800210463018644	30.829073962717978	20.61034275405893	21.76037282020445
9	28.081779915814792	12.935959110042091	17.705201443174985	41.27705953096813
10	18.889055923030668	23.846211665664462	29.94588093806374	27.31885147324113
11	39.1461214672279	19.40018039687312	18.96046301864101	22.493235117257967
12	22.372970535177387	24.56028262176789	26.608538785327724	26.458208057727
13	34.84666265784727	17.998346361996393	20.911004209260373	26.243986770895972
14	23.24113048707156	20.14807576668671	24.229555021046302	32.38123872519543
15	29.5625375826819	24.763229104028863	20.61034275405893	25.063890559230305
16	24.951142513529764	24.191972339146123	22.95174383644017	27.905141310883945
17	26.563439567047503	24.721888153938664	21.617558628983762	27.097113650030064
18	26.296602525556224	21.486019242333132	23.801112447384245	28.416265784726395
19	27.484215273601926	22.98932651834035	23.35012026458208	26.176337943475648
20	29.611395069152135	21.05006013229104	23.372669873722188	25.965874924834637
21	26.943024654239327	22.62101623571858	22.440619362597715	27.995339747444376
22	28.145670475045097	23.417769092002406	21.538634996993387	26.89792543595911
23	27.803668069753456	22.053517739025857	23.0419422730006	27.100871918220083
24	26.86410102224895	21.952044497895372	23.68084786530367	27.503006614552017
25	27.563138905592304	22.493235117257967	23.440318701142512	26.503307276007217
26	27.251202645820804	23.28998797354179	23.09831629585087	26.36049308478653
27	26.597263980757667	21.688965724594105	25.007516536380038	26.70625375826819
28	25.544948887552614	23.564341551413108	22.861545399879734	28.02916416115454
29	26.822760072158747	23.932651834034875	22.974293445580276	26.2702946482261
30	25.84561034275406	22.038484666265784	24.812086590499096	27.303818400481056
31	27.1873120865905	22.534576067348166	23.710914010823814	26.567197835237522
32	25.405892964521946	23.267438364401684	22.92543595911004	28.401232711966323
33	25.413409500901984	22.459410703547807	25.263078773301263	26.86410102224895
34	25.751653638003607	22.079825616355983	24.78577871316897	27.382742032471434
35	24.992483463619962	24.237071557426336	25.1879134095009	25.582531569452797
36	25.601322910402885	22.651082381238723	25.84561034275406	25.90198436560433
37	25.53367408298256	22.636049308478654	25.71782922429345	26.112447384245343
38	24.62041491280818	21.410853878532773	26.68370414912808	27.285027059530968
39	24.29344558027661	20.418671076368007	27.5217979555021	27.76608538785328
40	23.823662056524352	22.26022248947685	28.1870114251353	25.729104028863496
41	22.54585087191822	21.125225496091403	29.077720986169574	27.251202645820804
42	20.869663259170174	21.373271196632594	30.26909200240529	27.487973541791945
43	20.095460012026457	22.139957907396273	29.86319903788334	27.90138304269393
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	1.0
8	2.0
9	3.0
10	5.0
11	7.0
12	7.0
13	15.0
14	23.0
15	48.0
16	73.0
17	55.5
18	38.0
19	38.0
20	58.5
21	79.0
22	63.0
23	47.0
24	50.0
25	53.0
26	53.0
27	63.5
28	74.0
29	100.0
30	126.0
31	151.0
32	176.0
33	176.0
34	249.5
35	323.0
36	380.0
37	437.0
38	547.0
39	657.0
40	657.0
41	818.5
42	980.0
43	1165.0
44	1350.0
45	1804.0
46	2258.0
47	2258.0
48	2479.5
49	2701.0
50	3030.5
51	3360.0
52	3276.0
53	3192.0
54	3192.0
55	2808.0
56	2424.0
57	2288.0
58	2152.0
59	2017.0
60	1882.0
61	1882.0
62	1710.5
63	1539.0
64	1210.5
65	882.0
66	770.0
67	658.0
68	658.0
69	564.0
70	470.0
71	382.5
72	295.0
73	214.5
74	134.0
75	134.0
76	111.5
77	89.0
78	71.0
79	53.0
80	42.0
81	31.0
82	31.0
83	23.0
84	15.0
85	14.0
86	13.0
87	10.0
88	7.0
89	7.0
90	5.5
91	4.0
92	2.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	26608.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.2908899579074
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.41378388493824	61.44392663860493
2	6.426776452975458	9.034876728803368
3	2.2777094583756616	4.803066746843055
4	1.0265732770143827	2.8863499699338546
5	0.7111158637651713	2.4992483463619966
6	0.45981928032935887	1.9392663860493085
7	0.2887237341602951	1.420625375826819
8	0.26199005507137896	1.4732411304870716
9	0.17644228198684703	1.1162056524353579
>10	0.9142918248409346	11.50405892964522
>50	0.042773886542265944	1.8791340950090198
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	77	0.2893866506313891	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	71	0.26683704149128085	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	69	0.25932050511124477	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	63	0.2367708959711365	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	62	0.23301262778111848	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	54	0.20294648226097414	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	53	0.1991882140709561	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	51	0.19167167769092003	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	50	0.187913409500902	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	49	0.18415514131088395	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	48	0.1803968731208659	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	47	0.17663860493084788	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	45	0.1691220685508118	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	39	0.14657245941070354	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	39	0.14657245941070354	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.1428141912206855	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	37	0.13905592303066747	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	34	0.12778111846061335	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	34	0.12778111846061335	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	33	0.12402285027059531	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	32	0.12026458208057728	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	32	0.12026458208057728	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	31	0.11650631389055924	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	31	0.11650631389055924	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	30	0.1127480457005412	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	30	0.1127480457005412	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	29	0.10898977751052316	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	29	0.10898977751052316	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	29	0.10898977751052316	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	29	0.10898977751052316	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	28	0.10523150932050512	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	28	0.10523150932050512	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	28	0.10523150932050512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.00375826819001804	0.0
18	0.0	0.0	0.0	0.00375826819001804	0.0
19	0.0	0.0	0.0	0.00375826819001804	0.0
20	0.0	0.0	0.0	0.00375826819001804	0.0
21	0.0	0.0	0.0	0.00751653638003608	0.0
22	0.0	0.0	0.0	0.01127480457005412	0.0
23	0.0	0.0	0.0	0.01127480457005412	0.0
24	0.0	0.0	0.0	0.01127480457005412	0.0
25	0.0	0.0	0.0	0.01127480457005412	0.0
26	0.0	0.0	0.0	0.01127480457005412	0.0
27	0.0	0.0	0.0	0.05637402285027059	0.0
28	0.0	0.0	0.0	0.1991882140709561	0.0
29	0.0	0.0	0.0	0.3683102826217679	0.0
30	0.0	0.0	0.0	0.6464221286831028	0.0
31	0.0	0.0	0.0	1.3755261575466025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATACA	25	0.0054015527	29.6	37
GCTGCTG	25	0.0054015527	29.6	16
GGTATCA	60	3.99923E-8	27.750002	1
TCTTATA	65	2.5232057E-6	22.76923	37
GTATCAA	100	5.4565207E-6	16.650002	2
CTCTTAT	105	1.5618591E-4	14.095238	36
TATCAAC	135	9.273509E-5	12.333334	1
ATCAACG	135	9.273509E-5	12.333334	2
AACGCAG	140	1.3024594E-4	11.892858	5
TCAACGC	140	1.3024594E-4	11.892858	3
CAACGCA	140	1.3024594E-4	11.892858	4
TATCACC	110	0.003583109	11.772727	6
CTGTCTC	325	5.456968E-12	11.384615	37
ACGCAGA	150	2.4748954E-4	11.099999	6
CAGAGTA	150	2.4748954E-4	11.099999	9
AGAGTAC	150	2.4748954E-4	11.099999	10
GAGTACG	150	2.4748954E-4	11.099999	11
GCAGAGT	150	2.4748954E-4	11.099999	8
CGCAGAG	150	2.4748954E-4	11.099999	7
AGTACGG	155	3.353852E-4	10.741936	12
>>END_MODULE