FastQCFastQC Report
Fri 10 Feb 2017
ERR1631655.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631655.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597190
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA13450.2252214538086706No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13160.2203653778529446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.2049598955106415No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11310.18938696227331336No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11290.18905206048326328No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11220.18787990421808806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10840.18151677020713675No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10400.17414893082603528No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10330.17297677456086002No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9700.16242736817428288No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9640.1614226628041327No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9560.1600830556439324No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8820.1476916894120799No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8560.14333796614142905No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7920.13262110885982686No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7680.12860228737922605No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7570.12676032753395067No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7500.12558817126877542No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7040.11788543009762387No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6940.11621092114737354No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6840.1145364121971232No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6330.10599641655084646No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6090.10197759507024565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCT303.597426E-430.83333235
GGTATCA7200.030.576391
AACGATT809.767973E-1025.437522
CCGCTTA601.3356093E-624.66666625
TAAGGTA451.3221722E-424.6666665
TAAACCG400.001930106723.1255
CAACGAT1054.0017767E-1122.90476421
CAGTCGG1950.021.82051310
TACGTGA855.1702955E-821.76470636
GTACTGG3750.021.7066651
CCCAATA603.7224283E-521.5833322
CTTATAC7150.021.47552537
CCTACGT957.1268005E-921.42105334
AGTCGGT2000.021.27500211
AACAACG450.003823687620.55555525
TTCAACG1101.7498678E-920.18181819
GATTAGA656.895034E-519.9230772
CTACGTG951.6727427E-719.47368435
CTAAGTC1052.2539098E-819.38095334
CTCTATG3150.019.380951
Produced by FastQC (version 0.11.5)