##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631655.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	597190
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64291264086807	34.0	31.0	34.0	31.0	34.0
2	32.80838594082285	34.0	31.0	34.0	31.0	34.0
3	32.895781911954316	34.0	31.0	34.0	31.0	34.0
4	36.32126626366818	37.0	37.0	37.0	35.0	37.0
5	36.2367956596728	37.0	35.0	37.0	35.0	37.0
6	36.28898842914315	37.0	37.0	37.0	35.0	37.0
7	36.276873356888096	37.0	37.0	37.0	35.0	37.0
8	36.25397444699342	37.0	37.0	37.0	35.0	37.0
9	38.09323163482309	39.0	38.0	39.0	37.0	39.0
10	38.02778847602941	39.0	38.0	39.0	35.0	39.0
11	38.09520420636648	39.0	38.0	39.0	37.0	39.0
12	38.038051541385485	39.0	38.0	39.0	35.0	39.0
13	38.081704315209564	39.0	38.0	39.0	37.0	39.0
14	39.477506321271285	41.0	39.0	41.0	37.0	41.0
15	39.48506505467272	41.0	39.0	41.0	37.0	41.0
16	39.4431470721211	41.0	39.0	41.0	37.0	41.0
17	39.412352852526	41.0	39.0	41.0	37.0	41.0
18	39.43639210301579	41.0	39.0	41.0	37.0	41.0
19	39.46591202129975	41.0	39.0	41.0	37.0	41.0
20	39.45570756375693	41.0	39.0	41.0	37.0	41.0
21	39.42573720256535	41.0	39.0	41.0	37.0	41.0
22	39.40451782514778	40.0	39.0	41.0	37.0	41.0
23	39.341762253219244	40.0	39.0	41.0	36.0	41.0
24	39.34806845392588	41.0	39.0	41.0	36.0	41.0
25	39.292685744905306	40.0	39.0	41.0	36.0	41.0
26	39.236229675647614	40.0	39.0	41.0	36.0	41.0
27	39.132045077780944	40.0	39.0	41.0	36.0	41.0
28	39.12166814581624	40.0	39.0	41.0	36.0	41.0
29	39.10311291213851	40.0	39.0	41.0	35.0	41.0
30	39.05284247894305	40.0	39.0	41.0	35.0	41.0
31	38.97617843567374	40.0	38.0	41.0	35.0	41.0
32	38.925871163281364	40.0	38.0	41.0	35.0	41.0
33	38.87165391249016	40.0	38.0	41.0	35.0	41.0
34	38.840636983204675	40.0	38.0	41.0	35.0	41.0
35	38.7668731894372	40.0	38.0	41.0	35.0	41.0
36	38.71058289656558	40.0	38.0	41.0	35.0	41.0
37	38.67380063296439	40.0	38.0	41.0	35.0	41.0
38	38.63463051960013	40.0	38.0	41.0	35.0	41.0
39	38.57077646980023	40.0	38.0	41.0	35.0	41.0
40	38.240313802977276	40.0	38.0	41.0	34.0	41.0
41	38.32067516200874	40.0	38.0	41.0	34.0	41.0
42	38.30176158341566	40.0	37.0	41.0	34.0	41.0
43	37.830682027495435	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.0
17	2.0
18	6.0
19	7.0
20	21.0
21	44.0
22	79.0
23	162.0
24	295.0
25	426.0
26	731.0
27	1152.0
28	1772.0
29	2652.0
30	3907.0
31	5389.0
32	7152.0
33	9955.0
34	14380.0
35	21654.0
36	33506.0
37	61338.0
38	138932.0
39	293623.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.427870526967965	16.931127446876204	13.138029772769135	27.502972253386694
2	19.656223312513603	19.79704951522966	34.20703628660895	26.339690885647784
3	20.25469281133308	20.60650714178067	29.87658869036655	29.262211356519703
4	14.806343039903549	16.39243791758067	34.84401949128418	33.957199551231604
5	16.01098477871364	33.698990271103	34.050804601550595	16.239220348632763
6	32.780019759205615	34.98836216279576	16.0521776988898	16.179440379108826
7	29.824344011118743	28.718163398583364	20.543378154356233	20.91411443594166
8	26.754131850834746	32.125119308762706	20.463336626534268	20.657412213868284
9	28.423449823339308	13.270315979838912	18.135936636581324	40.170297560240456
10	18.844253922537217	24.11276143270986	30.16242736817428	26.880557276578642
11	38.0120229742628	20.479914265141748	19.489777122858722	22.018285637736735
12	22.418828178636616	24.35690483765636	27.359466836350236	25.864800147356785
13	33.81888511194092	18.303889884291433	22.029672298598435	25.847552705169207
14	23.55665701033172	20.457643296103416	24.364440127932486	31.621259565632375
15	28.853798623553644	24.69130427502135	21.034846531254708	25.4200505701703
16	24.64207371188399	24.91217200555937	23.149248982735813	27.29650529982083
17	25.933287563422024	24.50275456722316	22.75992565180261	26.804032217552205
18	26.03693966744252	22.260252181047907	24.208543344664175	27.494264806845393
19	27.468979721696613	22.90728243942464	24.17304375491887	25.45069408395988
20	28.97804718766222	21.368073812354528	23.955022689596277	25.69885631038698
21	26.543143723103203	23.03169845442824	23.10403724107905	27.321120581389508
22	27.383412314338816	23.65947185987709	22.32153920862707	26.63557661715702
23	26.993419179825516	23.06703059327852	23.53706525561379	26.40248497128217
24	26.70121736800683	22.49552068855808	23.9585391583918	26.844722785043285
25	26.888762370434872	22.946298497965472	24.217083340310456	25.947855791289204
26	26.87737570957317	23.639377752474086	23.406118655704216	26.07712788224853
27	26.473484150772787	22.641705319914934	24.63972939935364	26.245081129958642
28	25.5491552102346	23.780371406085166	23.150253688105963	27.520219695574273
29	26.35409166261994	23.9451430867898	23.395401798422615	26.30536345216765
30	26.01600830556439	23.147407022890537	24.48751653577588	26.349068135769187
31	26.88943217401497	23.216899144325925	23.5846213098009	26.309047371858203
32	24.602052947973007	23.29191714529714	23.954352886016174	28.151677020713677
33	25.019842931060467	22.747534285570755	25.719955123160133	26.512667660208646
34	25.538605803848018	22.584437113816374	24.9438202247191	26.933136857616503
35	24.97814765819923	24.241363720089083	25.42909291850165	25.351395703210034
36	25.32929218506673	23.30380615884392	25.591185384885883	25.775716271203468
37	25.825449187025907	22.952159279291347	25.035415864297793	26.186975669384953
38	25.11964366449539	22.257238064937457	25.78877744101542	26.834340829551735
39	24.687118002645725	21.33022991007887	26.762002042900917	27.22065004437449
40	24.428071468042	22.44394581289037	27.426447194360254	25.70153552470738
41	23.030191396373016	21.39654046450878	28.399839247140772	27.17342889197743
42	21.813660644016142	21.76108106297828	29.165257288300207	27.26000100470537
43	21.172993519650362	22.53453674709891	29.03832950987123	27.25414022337949
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	8.0
2	9.0
3	24.5
4	40.0
5	40.0
6	63.5
7	87.0
8	97.0
9	107.0
10	166.0
11	225.0
12	225.0
13	433.0
14	641.0
15	1379.0
16	2117.0
17	2099.0
18	2081.0
19	2081.0
20	2269.5
21	2458.0
22	2153.5
23	1849.0
24	1989.5
25	2130.0
26	2130.0
27	2439.0
28	2748.0
29	3566.5
30	4385.0
31	5329.0
32	6273.0
33	6273.0
34	7847.5
35	9422.0
36	10740.5
37	12059.0
38	14613.5
39	17168.0
40	17168.0
41	20109.5
42	23051.0
43	26402.0
44	29753.0
45	39348.5
46	48944.0
47	48944.0
48	53404.0
49	57864.0
50	63537.0
51	69210.0
52	69545.0
53	69880.0
54	69880.0
55	62904.5
56	55929.0
57	51752.5
58	47576.0
59	42881.5
60	38187.0
61	38187.0
62	34312.0
63	30437.0
64	25248.5
65	20060.0
66	17195.5
67	14331.0
68	14331.0
69	12143.5
70	9956.0
71	8523.0
72	7090.0
73	5763.5
74	4437.0
75	4437.0
76	3528.5
77	2620.0
78	2185.0
79	1750.0
80	1373.5
81	997.0
82	997.0
83	801.5
84	606.0
85	485.0
86	364.0
87	279.0
88	194.0
89	194.0
90	147.0
91	100.0
92	68.0
93	36.0
94	22.5
95	9.0
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	597190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.14755329850095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.46822035586624	41.150856949120936
2	6.778805643422935	6.527658120337695
3	2.3258370986052537	3.359500970061818
4	1.184811725750119	2.2818314285697094
5	0.7582173431917131	1.8253154971585397
6	0.5267360515553805	1.5216631269902776
7	0.37562541684339074	1.2659811344417156
8	0.2987168576646504	1.1505988660455582
9	0.2280347695273479	0.9881384597756427
>10	1.7158260873949942	16.687298267536736
>50	0.19860597267605268	6.673135214613756
>100	0.127974975039139	11.733795280509687
>500	0.009440776847149597	3.0793257980975453
>1k	0.0031469256157165325	1.7549008867404083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1345	0.2252214538086706	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1316	0.2203653778529446	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1224	0.2049598955106415	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1131	0.18938696227331336	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1129	0.18905206048326328	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1122	0.18787990421808806	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1084	0.18151677020713675	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1040	0.17414893082603528	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1033	0.17297677456086002	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	970	0.16242736817428288	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	964	0.1614226628041327	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	956	0.1600830556439324	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	882	0.1476916894120799	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	856	0.14333796614142905	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	792	0.13262110885982686	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	768	0.12860228737922605	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	757	0.12676032753395067	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	750	0.12558817126877542	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	704	0.11788543009762387	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	694	0.11621092114737354	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	684	0.1145364121971232	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	633	0.10599641655084646	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	609	0.10197759507024565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.674508950250339E-4	0.0	0.0	0.0	0.0
3	1.674508950250339E-4	0.0	0.0	0.0	0.0
4	1.674508950250339E-4	0.0	0.0	0.0	0.0
5	1.674508950250339E-4	0.0	0.0	0.0	0.0
6	1.674508950250339E-4	0.0	0.0	0.0	0.0
7	3.349017900500678E-4	0.0	0.0	0.0	0.0
8	5.023526850751018E-4	0.0	0.0	0.0	0.0
9	5.023526850751018E-4	0.0	0.0	0.0	0.0
10	5.023526850751018E-4	0.0	0.0	0.0	0.0
11	5.023526850751018E-4	0.0	0.0	1.674508950250339E-4	0.0
12	5.023526850751018E-4	0.0	0.0	1.674508950250339E-4	0.0
13	5.023526850751018E-4	0.0	0.0	3.349017900500678E-4	0.0
14	5.023526850751018E-4	0.0	0.0	5.023526850751018E-4	0.0
15	5.023526850751018E-4	0.0	0.0	6.698035801001356E-4	0.0
16	6.698035801001356E-4	0.0	0.0	0.0015070580552253052	0.0
17	6.698035801001356E-4	0.0	0.0	0.0028466652154255766	0.0
18	8.372544751251695E-4	0.0	0.0	0.004018821480600814	0.0
19	8.372544751251695E-4	0.0	0.0	0.005860781325876187	0.0
20	8.372544751251695E-4	0.0	0.0	0.007535290276126526	0.0
21	8.372544751251695E-4	0.0	0.0	0.011889013546777407	0.0
22	8.372544751251695E-4	0.0	0.0	0.017414893082603525	0.0
23	8.372544751251695E-4	0.0	0.0	0.021266263668179308	0.0
24	8.372544751251695E-4	0.0	0.0	0.025619986938830188	0.0
25	8.372544751251695E-4	0.0	0.0	0.02695959409903046	0.0
26	8.372544751251695E-4	0.0	0.0	0.030141161104506105	0.0
27	8.372544751251695E-4	0.0	0.0	0.04420703628660895	0.0
28	8.372544751251695E-4	0.0	0.0	0.11319680503692292	0.0
29	8.372544751251695E-4	0.0	0.0	0.23727791825047304	0.0
30	8.372544751251695E-4	0.0	0.0	0.404896264170532	0.0
31	8.372544751251695E-4	0.0	0.0	0.9728897000954471	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGCT	30	3.597426E-4	30.833332	35
GGTATCA	720	0.0	30.57639	1
AACGATT	80	9.767973E-10	25.4375	22
CCGCTTA	60	1.3356093E-6	24.666666	25
TAAGGTA	45	1.3221722E-4	24.666666	5
TAAACCG	40	0.0019301067	23.125	5
CAACGAT	105	4.0017767E-11	22.904764	21
CAGTCGG	195	0.0	21.820513	10
TACGTGA	85	5.1702955E-8	21.764706	36
GTACTGG	375	0.0	21.706665	1
CCCAATA	60	3.7224283E-5	21.583332	2
CTTATAC	715	0.0	21.475525	37
CCTACGT	95	7.1268005E-9	21.421053	34
AGTCGGT	200	0.0	21.275002	11
AACAACG	45	0.0038236876	20.555555	25
TTCAACG	110	1.7498678E-9	20.181818	19
GATTAGA	65	6.895034E-5	19.923077	2
CTACGTG	95	1.6727427E-7	19.473684	35
CTAAGTC	105	2.2539098E-8	19.380953	34
CTCTATG	315	0.0	19.38095	1
>>END_MODULE