FastQCFastQC Report
Fri 10 Feb 2017
ERR1631654.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631654.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689286
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16170.2345905763355124No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14860.21558540286615424No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12930.18758541447236704No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12910.18729525915222417No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12630.18323308467022398No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12570.18236261870979537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12570.18236261870979537No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12480.18105691976915245No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10420.15117092179443656No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10370.15044553349407938No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10140.14710874731243634No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9830.14261133985022184No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9760.14159579622972177No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA8770.1272331078826496No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8540.12389632170100656No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8470.12288077808050649No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7750.1124351865553631No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7730.11214503123522022No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7720.11199995357514879No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7520.10909840037372005No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC7260.10532638121186272No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7070.10256990567050542No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6960.10097405140971963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10550.030.862561
GTGTAAT250.005494836729.5999981
TATAATC250.005494836729.5999983
TTTTACG809.767973E-1025.43754
GACCGTT551.9012261E-523.5454547
GTACCTA400.001930407423.12534
TTACGAG903.8216967E-922.6111136
CGAACTA502.7005823E-422.19999924
ATACACA1350.021.92592637
TTAGGCA603.7235015E-521.5833324
GTTCCGA705.0970357E-621.14285733
GGACCGT1151.3460522E-1020.9130446
AATGCAA450.003824276820.55555724
CGGGACG450.003824276820.55555718
CTTATAC7400.020.537
GCCTAGT656.897007E-519.9230771
GTATCAA16850.019.2136522
TAGCGAA701.2182991E-418.510
AGGACCG902.1496726E-618.55
TACGAGG1103.8449798E-818.57
Produced by FastQC (version 0.11.5)