##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631654.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689286
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.599315813755105	34.0	31.0	34.0	31.0	34.0
2	32.768020821545775	34.0	31.0	34.0	31.0	34.0
3	32.8527664858999	34.0	31.0	34.0	31.0	34.0
4	36.28743221246333	37.0	37.0	37.0	35.0	37.0
5	36.20454354215812	37.0	35.0	37.0	35.0	37.0
6	36.255947458674626	37.0	37.0	37.0	35.0	37.0
7	36.24958000017409	37.0	37.0	37.0	35.0	37.0
8	36.23290477392548	37.0	37.0	37.0	35.0	37.0
9	38.05698505409946	39.0	38.0	39.0	35.0	39.0
10	37.993668810914485	39.0	38.0	39.0	35.0	39.0
11	38.06323790124854	39.0	38.0	39.0	37.0	39.0
12	38.0012084969084	39.0	38.0	39.0	35.0	39.0
13	38.05367873422643	39.0	38.0	39.0	35.0	39.0
14	39.43904562112099	40.0	39.0	41.0	37.0	41.0
15	39.442588417579934	40.0	39.0	41.0	37.0	41.0
16	39.39382781602992	40.0	39.0	41.0	37.0	41.0
17	39.37333124421503	40.0	39.0	41.0	37.0	41.0
18	39.39495942177848	40.0	39.0	41.0	37.0	41.0
19	39.41336977684154	40.0	39.0	41.0	37.0	41.0
20	39.41756542277081	40.0	39.0	41.0	37.0	41.0
21	39.37601227937315	40.0	39.0	41.0	36.0	41.0
22	39.3551979874827	40.0	39.0	41.0	36.0	41.0
23	39.28888298906405	40.0	39.0	41.0	36.0	41.0
24	39.29905728536485	40.0	39.0	41.0	36.0	41.0
25	39.24852093325557	40.0	39.0	41.0	36.0	41.0
26	39.183739405703875	40.0	39.0	41.0	36.0	41.0
27	39.07905716930273	40.0	39.0	41.0	35.0	41.0
28	39.06809510130773	40.0	39.0	41.0	35.0	41.0
29	39.04951500538238	40.0	39.0	41.0	35.0	41.0
30	38.998440415154235	40.0	38.0	41.0	35.0	41.0
31	38.91005185075571	40.0	38.0	41.0	35.0	41.0
32	38.84952690175051	40.0	38.0	41.0	35.0	41.0
33	38.79774433254121	40.0	38.0	41.0	35.0	41.0
34	38.76756527769315	40.0	38.0	41.0	35.0	41.0
35	38.690691527174494	40.0	38.0	41.0	35.0	41.0
36	38.63685320752199	40.0	38.0	41.0	35.0	41.0
37	38.59339954677739	40.0	38.0	41.0	35.0	41.0
38	38.55517738645497	40.0	38.0	41.0	35.0	41.0
39	38.48204373801296	40.0	38.0	41.0	35.0	41.0
40	38.1594229390993	40.0	38.0	41.0	34.0	41.0
41	38.23714829548257	40.0	37.0	41.0	34.0	41.0
42	38.21471348612913	40.0	37.0	41.0	34.0	41.0
43	37.74523202270175	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	4.0
19	8.0
20	37.0
21	50.0
22	93.0
23	167.0
24	315.0
25	549.0
26	858.0
27	1449.0
28	2179.0
29	3294.0
30	4616.0
31	6303.0
32	8587.0
33	11872.0
34	17383.0
35	25975.0
36	40625.0
37	73240.0
38	165175.0
39	326505.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.49556787748482	16.998459275250042	13.277507449737843	27.228465397527295
2	20.0176704589967	19.958333696027484	33.91814138108129	26.105854463894524
3	20.765545796664956	20.490913786149726	29.97580104630008	28.767739370885238
4	15.104035190037226	16.632863571870022	34.48118777981854	33.78191345827422
5	16.326024320818934	33.59548866508242	33.641478283325064	16.437008730773584
6	32.674825834269086	34.565332822659975	16.22592073536964	16.5339206077013
7	29.380982640007197	28.850723792446097	20.474374932901583	21.293918634645127
8	26.64032056359769	31.75619409069675	20.534872317151372	21.068613028554182
9	27.93571899037555	13.651372579741935	18.428925003554404	39.98398342632811
10	19.01547398322322	24.318352614154357	30.11333466804781	26.552838734574618
11	37.68638852377678	20.578975925813086	19.736364876118188	21.99827067429195
12	22.208923436715676	24.629689272667658	27.479739904771023	25.68164738584564
13	34.07787188481994	18.271080509396683	22.011617819018518	25.639429786764858
14	23.438456605821095	20.782955115873527	24.359699747274714	31.41888853103066
15	28.68823681316609	25.07116059226504	21.128094869183474	25.112507725385395
16	24.378995076064218	25.011533673975677	23.386954036495737	27.22251721346437
17	25.712984160421072	24.684963861154877	22.987265082998928	26.61478689542512
18	25.704134423156717	22.347762757404038	24.434559819871577	27.51354299956767
19	27.21236177725937	23.312239041558946	24.56991727671823	24.905481904463457
20	28.704195355773948	21.75497543835215	24.143098800788064	25.397730405085845
21	26.49335689394533	23.337772709731517	23.128425646248438	27.040444750074716
22	27.007076888258286	23.8290056667334	22.746726322600487	26.417191122407825
23	26.68950189036191	23.138436004793366	23.752839895195898	26.419222209648822
24	26.79236195135256	22.6405294754282	24.091015920822418	26.476092652396826
25	26.761605487417416	23.003078547946714	24.435575363492077	25.799740601143796
26	26.68427909459934	23.61951352559025	23.7873683782929	25.908839001517514
27	26.145605742754096	22.75194911836306	25.019367867619536	26.083077271263306
28	25.332010225073482	23.91996935959819	23.620674146870822	27.127346268457504
29	26.090331154266877	24.43310904327086	23.529565376345957	25.946994426116298
30	25.793212106440578	23.556839976439388	24.793336873228238	25.856611043891796
31	26.82993706531106	23.260446316913445	23.99149264601341	25.918123971762085
32	24.555699666031227	23.42278821853338	24.31037334285043	27.711138772584963
33	24.829461210586025	22.901814341216852	25.896942633391657	26.371781814805466
34	25.256424764176266	22.807658939830493	25.272528384444193	26.66338791154905
35	24.9453782609831	24.10131643468749	25.50044538841642	25.45285991591299
36	25.10409322110125	23.11943083132401	25.924072155825012	25.852403791749722
37	25.743450469036077	22.606871458291625	25.50465264055849	26.145025432113812
38	24.77012444761681	22.07733799903088	26.52353304724019	26.629004506112118
39	24.307471789649	21.225151823771267	27.585501518963103	26.881874867616634
40	24.090870843162346	22.162788740812957	28.167408013509633	25.578932402515065
41	22.624280777500196	21.53924495782592	29.10693094013225	26.729543324541627
42	21.535618016324136	21.955472764570874	29.57350069492199	26.935408524182996
43	20.602043273764444	22.610788555113555	29.418122520985484	27.36904565013652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14.0
1	13.0
2	12.0
3	37.0
4	62.0
5	62.0
6	82.0
7	102.0
8	116.5
9	131.0
10	224.5
11	318.0
12	318.0
13	622.0
14	926.0
15	1947.5
16	2969.0
17	2883.0
18	2797.0
19	2797.0
20	3134.0
21	3471.0
22	3040.0
23	2609.0
24	2810.0
25	3011.0
26	3011.0
27	3365.5
28	3720.0
29	5016.5
30	6313.0
31	7257.0
32	8201.0
33	8201.0
34	9825.0
35	11449.0
36	12714.5
37	13980.0
38	17267.5
39	20555.0
40	20555.0
41	24360.5
42	28166.0
43	31354.0
44	34542.0
45	45122.5
46	55703.0
47	55703.0
48	61193.0
49	66683.0
50	73170.0
51	79657.0
52	79869.0
53	80081.0
54	80081.0
55	70923.0
56	61765.0
57	57029.5
58	52294.0
59	47776.0
60	43258.0
61	43258.0
62	38855.5
63	34453.0
64	28546.5
65	22640.0
66	19649.0
67	16658.0
68	16658.0
69	14143.5
70	11629.0
71	9855.0
72	8081.0
73	6544.0
74	5007.0
75	5007.0
76	4118.5
77	3230.0
78	2634.0
79	2038.0
80	1643.0
81	1248.0
82	1248.0
83	984.0
84	720.0
85	570.5
86	421.0
87	312.0
88	203.0
89	203.0
90	169.0
91	135.0
92	81.0
93	27.0
94	17.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	689286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.45357665225181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.55218909638285	41.9376312880178
2	6.383404993440206	6.185976063040441
3	2.093184744040736	3.042668623281057
4	1.0683349724352629	2.0705860190869334
5	0.6727187858913439	1.6297815628798002
6	0.44971991001012057	1.3074322879031497
7	0.3423243438258244	1.161078718344747
8	0.2830549604469708	1.0972020178253925
9	0.2060323098922289	0.8984702088183252
>10	1.5815809276341681	15.450975091979927
>50	0.22023261623514856	7.3657959108332065
>100	0.13430282831640306	12.682253124048861
>500	0.009614520147930333	3.160409034838723
>1k	0.0033049913008510517	2.0097400491016892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1617	0.2345905763355124	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1486	0.21558540286615424	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1293	0.18758541447236704	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1291	0.18729525915222417	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1263	0.18323308467022398	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1257	0.18236261870979537	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1257	0.18236261870979537	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1248	0.18105691976915245	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1042	0.15117092179443656	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1037	0.15044553349407938	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1014	0.14710874731243634	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	983	0.14261133985022184	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	976	0.14159579622972177	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	877	0.1272331078826496	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	854	0.12389632170100656	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	847	0.12288077808050649	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	775	0.1124351865553631	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	773	0.11214503123522022	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	772	0.11199995357514879	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	752	0.10909840037372005	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	726	0.10532638121186272	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	707	0.10256990567050542	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	696	0.10097405140971963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.4507766007143625E-4	0.0
13	0.0	0.0	0.0	1.4507766007143625E-4	0.0
14	0.0	0.0	0.0	1.4507766007143625E-4	0.0
15	2.901553201428725E-4	0.0	0.0	1.4507766007143625E-4	0.0
16	2.901553201428725E-4	0.0	0.0	0.001305698940642926	0.0
17	2.901553201428725E-4	0.0	0.0	0.001740931920857235	0.0
18	2.901553201428725E-4	0.0	0.0	0.0020310872410001075	0.0
19	2.901553201428725E-4	0.0	0.0	0.003046630861500161	0.0
20	2.901553201428725E-4	0.0	0.0	0.00348186384171447	0.0
21	2.901553201428725E-4	0.0	0.0	0.005658028742786013	0.0
22	2.901553201428725E-4	0.0	0.0	0.008704659604286175	0.0
23	2.901553201428725E-4	0.0	0.0	0.010155436205000536	0.0
24	2.901553201428725E-4	0.0	0.0	0.013782377706786442	0.0
25	2.901553201428725E-4	0.0	0.0	0.015378231967572242	0.0
26	2.901553201428725E-4	0.0	0.0	0.017989629848858095	0.0
27	2.901553201428725E-4	0.0	0.0	0.036124337357787625	0.0
28	2.901553201428725E-4	0.0	0.0	0.12679787490243527	0.0
29	2.901553201428725E-4	0.0	0.0	0.2752123211555145	0.0
30	2.901553201428725E-4	0.0	0.0	0.4810775207968826	0.0
31	2.901553201428725E-4	0.0	0.0	1.0912741590573434	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1055	0.0	30.86256	1
GTGTAAT	25	0.0054948367	29.599998	1
TATAATC	25	0.0054948367	29.599998	3
TTTTACG	80	9.767973E-10	25.4375	4
GACCGTT	55	1.9012261E-5	23.545454	7
GTACCTA	40	0.0019304074	23.125	34
TTACGAG	90	3.8216967E-9	22.611113	6
CGAACTA	50	2.7005823E-4	22.199999	24
ATACACA	135	0.0	21.925926	37
TTAGGCA	60	3.7235015E-5	21.583332	4
GTTCCGA	70	5.0970357E-6	21.142857	33
GGACCGT	115	1.3460522E-10	20.913044	6
AATGCAA	45	0.0038242768	20.555557	24
CGGGACG	45	0.0038242768	20.555557	18
CTTATAC	740	0.0	20.5	37
GCCTAGT	65	6.897007E-5	19.923077	1
GTATCAA	1685	0.0	19.213652	2
TAGCGAA	70	1.2182991E-4	18.5	10
AGGACCG	90	2.1496726E-6	18.5	5
TACGAGG	110	3.8449798E-8	18.5	7
>>END_MODULE