FastQCFastQC Report
Fri 10 Feb 2017
ERR1631653.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631653.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649983
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC15730.24200632939630728No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12100.1861587149202364No Hit
GTGCAGGAGGCGGCGGGTGTGGGGCTGCCTGCAGGCTGCGTCT11880.18277401101259572No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11740.18062010852591528No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11200.17231219893443367No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11140.17138909786871348No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10190.15677333099481064No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10180.1566194808171906No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9570.14723461998236878No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8860.13631125737134664No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC8670.13338810399656606No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8550.13154190186512568No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT8350.12846489831272512No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG8040.12369554280650416No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8010.1232339922736441No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT7830.12046468907648353No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA7710.11861848694504319No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7670.11800308623456306No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT7320.112618330017862No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG7000.10769512433402104No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT6770.10415657024876036No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT200.001841347437.029
GGTATCA8050.029.645961
TCGTATG250.00549460829.630
TAAGAGT250.00549460829.64
GTTTATA358.865233E-426.428571
CGTTAGA551.9010095E-523.5454541
GTAACAC400.00193028923.1250023
TCAAGCC652.679868E-622.7692331
TATTAGA1201.0913936E-1121.5833322
ATTAGAG1151.3460522E-1020.9130443
AACGACC1151.3460522E-1020.91304437
ATACACA1151.3460522E-1020.91304437
TCCAGGA5050.020.881192
TTCGCTA450.00382404720.55555526
CCAGGAC5550.020.3333343
GACAGGC4550.020.3296727
GTACCAT555.139788E-420.1818186
ACCGCTA555.139788E-420.18181822
GATCTCT656.896233E-519.9230776
TTAGAAC759.25562E-619.7333323
Produced by FastQC (version 0.11.5)