FastQCFastQC Report
Fri 10 Feb 2017
ERR1631652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences519519
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12540.24137711998983677No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11610.22347594601929863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10170.19575799922620735No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8760.1686175096579721No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8670.16688513798340388No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8100.15591345071113857No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6730.1295429041093781No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6560.12627064650186037No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5890.11337410181340817No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5720.11010184420589045No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5580.1074070438232288No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5540.10663710085675403No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5500.10586715789027928No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5350.10297987176599893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATCG200.001841007537.011
TCGATCA303.596786E-430.83333416
GGTATCA7000.030.1285721
TGCCTTA250.005493605529.612
GGGCTAA509.078571E-625.9000021
TACGCCA400.001929770223.1250025
CTCTAAT652.678149E-622.7692281
AGAGGTC1400.022.4642858
GATTTAT502.699341E-422.21
ATACGGC603.7212267E-521.58333429
TAAACCG450.003823028920.5555555
CTTAACG555.1378796E-420.18181817
GATGCTA555.1378796E-420.18181814
GTCTTGG555.1378796E-420.1818181
TCGCCAT1202.382876E-1020.04166813
ATTAGCT1202.382876E-1020.0416683
CTTATAC7400.019.7537
TCTAATA759.24975E-619.7333342
TAATGTC759.24975E-619.7333347
ACGGTAC851.2426208E-619.5882363
Produced by FastQC (version 0.11.5)