##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631652.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	519519
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65268065268065	34.0	31.0	34.0	31.0	34.0
2	32.82343475407059	34.0	31.0	34.0	31.0	34.0
3	32.90701976251109	34.0	31.0	34.0	31.0	34.0
4	36.321535882229526	37.0	37.0	37.0	35.0	37.0
5	36.2447379210385	37.0	37.0	37.0	35.0	37.0
6	36.298924582161575	37.0	37.0	37.0	35.0	37.0
7	36.28710018305394	37.0	37.0	37.0	35.0	37.0
8	36.27197657833496	37.0	37.0	37.0	35.0	37.0
9	38.09703206234998	39.0	38.0	39.0	37.0	39.0
10	38.04710318583151	39.0	38.0	39.0	35.0	39.0
11	38.10647156311896	39.0	38.0	39.0	37.0	39.0
12	38.05311451554226	39.0	38.0	39.0	35.0	39.0
13	38.099116682931715	39.0	38.0	39.0	37.0	39.0
14	39.507313495752804	41.0	39.0	41.0	37.0	41.0
15	39.51287440882817	41.0	39.0	41.0	37.0	41.0
16	39.460776218001655	41.0	39.0	41.0	37.0	41.0
17	39.44163543585509	41.0	39.0	41.0	37.0	41.0
18	39.46404655075175	41.0	39.0	41.0	37.0	41.0
19	39.494728777965776	41.0	39.0	41.0	37.0	41.0
20	39.4855991792408	41.0	39.0	41.0	37.0	41.0
21	39.449132755491135	41.0	39.0	41.0	37.0	41.0
22	39.42411923336779	41.0	39.0	41.0	37.0	41.0
23	39.3552690084482	41.0	39.0	41.0	36.0	41.0
24	39.37889855808931	41.0	39.0	41.0	36.0	41.0
25	39.31804996544881	41.0	39.0	41.0	36.0	41.0
26	39.269077743066184	40.0	39.0	41.0	36.0	41.0
27	39.16163605180946	40.0	39.0	41.0	36.0	41.0
28	39.15790182842206	40.0	39.0	41.0	36.0	41.0
29	39.12691547373628	40.0	39.0	41.0	35.0	41.0
30	39.07839944256129	40.0	39.0	41.0	35.0	41.0
31	38.993723039965815	40.0	39.0	41.0	35.0	41.0
32	38.94137654253261	40.0	38.0	41.0	35.0	41.0
33	38.88279543192838	40.0	38.0	41.0	35.0	41.0
34	38.85131438888664	40.0	38.0	41.0	35.0	41.0
35	38.783898182742114	40.0	38.0	41.0	35.0	41.0
36	38.7380192062273	40.0	38.0	41.0	35.0	41.0
37	38.696696367216596	40.0	38.0	41.0	35.0	41.0
38	38.66150227421904	40.0	38.0	41.0	35.0	41.0
39	38.59551816199215	40.0	38.0	41.0	35.0	41.0
40	38.25181947147265	40.0	38.0	41.0	34.0	41.0
41	38.3394178076259	40.0	38.0	41.0	34.0	41.0
42	38.323926555140424	40.0	38.0	41.0	34.0	41.0
43	37.859135084568614	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	3.0
17	2.0
18	4.0
19	7.0
20	21.0
21	38.0
22	79.0
23	126.0
24	235.0
25	419.0
26	609.0
27	992.0
28	1581.0
29	2358.0
30	3304.0
31	4559.0
32	6225.0
33	8386.0
34	12246.0
35	18409.0
36	28709.0
37	53136.0
38	118468.0
39	259603.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.28661511898506	17.47116082376198	13.356393125179252	26.885830932073706
2	19.647212132761265	20.210232926995932	34.61682825844676	25.525726681796044
3	20.405605954738903	20.969589177681662	30.238355093846426	28.386449773733013
4	15.021587275922535	16.613251873367478	34.54252876218194	33.822632088528046
5	16.18439364104104	33.751797335612366	33.86844369503329	16.195365328313304
6	32.628258061784074	34.843191490590335	16.127995318746763	16.40055512887883
7	29.119435477816978	29.315385962784806	20.66584667740737	20.89933188199084
8	26.30105924903613	32.4956353858088	20.489144766601413	20.71416059855366
9	27.511409592334445	14.09707825892797	18.806819384854066	39.58469276388352
10	18.72867017375688	24.794665835128264	30.618129462060097	25.85853452905476
11	36.86101951997906	21.09316502380086	20.35055503263596	21.695260423584124
12	22.09505330892614	24.83893755570056	27.75644394141504	25.309565193958257
13	33.095998413917485	18.822025758441942	22.579347434838766	25.502628392801803
14	23.189142264286772	21.117418227244816	24.5128667093985	31.18057279906991
15	28.10599804819458	25.411005179791307	21.667349991049413	24.815646780964702
16	24.433177612368365	25.441610412708677	23.65823001661152	26.466981958311436
17	25.34479008467448	25.04778458535684	23.490767421403262	26.116657908565422
18	25.172130374442514	23.039003385824195	25.039315212725615	26.749551027007673
19	26.873319358868493	23.63320687020109	24.997353321052742	24.496120449877676
20	27.864235956721505	22.436523014557697	24.642217127766262	25.05702390095454
21	25.869698702068643	24.04974601506393	23.74061391402432	26.3399413688431
22	26.42251775199752	24.37658680433247	23.263441760551586	25.937453683118424
23	26.150920370573548	23.861302474019237	24.38139894787294	25.606378207534274
24	26.301444220519365	23.383360377580033	24.358685630361933	25.95650977153867
25	26.27160893056847	23.66073233125256	24.66878016010964	25.39887857806933
26	26.083550361007006	24.075731590182457	24.2924705352451	25.548247513565432
27	25.744775455758113	23.398759236909523	25.272992903050707	25.583472404281654
28	24.935757883734762	24.43548744126779	24.214321324147914	26.414433350849535
29	25.629861468011754	24.951349228805878	24.134439741376156	25.28434956180621
30	25.250857042764558	24.104796937166878	25.21101249424949	25.433333525819073
31	25.925519567138068	23.81067872397352	24.63778995570903	25.626011753179384
32	23.965629745976567	23.870349303875315	25.032578211768964	27.131442738379153
33	24.396027864235954	23.244770643614572	26.431179610370364	25.928021881779106
34	24.706507365466905	23.206850952515694	25.897416648861736	26.189225033155665
35	24.47629441849095	24.197575064627088	25.91300799393285	25.41312252294911
36	24.59582806403616	23.30540365222446	26.605186720793654	25.493581562945728
37	24.966555602393754	22.510052567856036	26.23330426798635	26.290087561763865
38	24.015676038797427	22.194760923084623	27.26445038583767	26.52511265228028
39	23.554865173362284	21.375349121013862	28.506560876503073	26.563224829120784
40	23.364881746384636	22.251929188345372	28.943503509977496	25.439685555292492
41	22.045969444813377	21.693720537651174	29.95867331127447	26.301636706260982
42	20.989607694810005	22.10102036691632	30.295330873365558	26.61404106490812
43	20.344972946129015	22.677707648805914	30.02546586361615	26.951853541448916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	20.0
1	19.5
2	19.0
3	47.5
4	76.0
5	76.0
6	101.0
7	126.0
8	135.0
9	144.0
10	235.5
11	327.0
12	327.0
13	640.0
14	953.0
15	1730.0
16	2507.0
17	2532.0
18	2557.0
19	2557.0
20	2823.0
21	3089.0
22	2886.0
23	2683.0
24	2882.0
25	3081.0
26	3081.0
27	3461.5
28	3842.0
29	5109.0
30	6376.0
31	7310.5
32	8245.0
33	8245.0
34	9657.0
35	11069.0
36	12097.5
37	13126.0
38	15727.5
39	18329.0
40	18329.0
41	21356.0
42	24383.0
43	26304.5
44	28226.0
45	34632.5
46	41039.0
47	41039.0
48	44702.5
49	48366.0
50	52612.0
51	56858.0
52	56848.5
53	56839.0
54	56839.0
55	50376.5
56	43914.0
57	40250.5
58	36587.0
59	33090.0
60	29593.0
61	29593.0
62	26981.0
63	24369.0
64	20327.0
65	16285.0
66	14156.5
67	12028.0
68	12028.0
69	10278.5
70	8529.0
71	7342.5
72	6156.0
73	4976.5
74	3797.0
75	3797.0
76	3084.0
77	2371.0
78	1930.0
79	1489.0
80	1198.0
81	907.0
82	907.0
83	738.0
84	569.0
85	465.5
86	362.0
87	263.5
88	165.0
89	165.0
90	125.0
91	85.0
92	56.5
93	28.0
94	16.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	519519.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.3338257994728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.09907456973679	45.58227795827896
2	6.030965862713801	6.3124703372366255
3	2.0077392522250026	3.152180288301212
4	1.0256595930017924	2.147067618788559
5	0.634371325438099	1.659953921882907
6	0.4625881293578391	1.4525403947230282
7	0.35535242525554267	1.3017866344520626
8	0.27759651576350214	1.1622150158806182
9	0.20583701977612895	0.9695014862441913
>10	1.5936142066233938	16.689056695372496
>50	0.18987248719926159	6.789921307877058
>100	0.11140666399231682	10.515719683597538
>500	0.00481158349468478	1.6005340261483259
>1k	0.0011103654218503336	0.6647746312163928
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1254	0.24137711998983677	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1161	0.22347594601929863	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.19575799922620735	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	876	0.1686175096579721	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	867	0.16688513798340388	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	810	0.15591345071113857	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	673	0.1295429041093781	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	656	0.12627064650186037	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	589	0.11337410181340817	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	572	0.11010184420589045	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	558	0.1074070438232288	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	554	0.10663710085675403	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	550	0.10586715789027928	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	535	0.10297987176599893	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.924857416186896E-4	0.0	0.0	0.0	0.0
9	1.924857416186896E-4	0.0	0.0	0.0	0.0
10	3.849714832373792E-4	0.0	0.0	3.849714832373792E-4	0.0
11	3.849714832373792E-4	0.0	0.0	3.849714832373792E-4	0.0
12	3.849714832373792E-4	0.0	0.0	5.774572248560687E-4	0.0
13	3.849714832373792E-4	0.0	0.0	5.774572248560687E-4	0.0
14	3.849714832373792E-4	0.0	0.0	9.62428708093448E-4	0.0
15	3.849714832373792E-4	0.0	0.0	0.0011549144497121375	0.0
16	3.849714832373792E-4	0.0	0.0	0.0021173431578055857	0.0
17	3.849714832373792E-4	0.0	0.0	0.0025023146410429646	0.0
18	3.849714832373792E-4	0.0	0.0	0.002887286124280344	0.0
19	3.849714832373792E-4	0.0	0.0	0.0036572290907551023	0.0
20	3.849714832373792E-4	0.0	0.0	0.004234686315611171	0.0
21	3.849714832373792E-4	0.0	0.0	0.005389600765323309	0.0
22	3.849714832373792E-4	0.0	0.0	0.008276886889603652	0.0
23	3.849714832373792E-4	0.0	0.0	0.00962428708093448	0.0
24	3.849714832373792E-4	0.0	0.0	0.011549144497121376	0.0
25	3.849714832373792E-4	0.0	0.0	0.013666487654926961	0.0
26	3.849714832373792E-4	0.0	0.0	0.01809365971215682	0.0
27	3.849714832373792E-4	0.0	0.0	0.04658154947172288	0.0
28	3.849714832373792E-4	0.0	0.0	0.16188050870131795	0.0
29	3.849714832373792E-4	0.0	0.0	0.34705179213849735	0.0
30	3.849714832373792E-4	0.0	0.0	0.5834242828462481	0.0
31	3.849714832373792E-4	0.0	0.0	1.32641924549439	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTATCG	20	0.0018410075	37.0	11
TCGATCA	30	3.596786E-4	30.833334	16
GGTATCA	700	0.0	30.128572	1
TGCCTTA	25	0.0054936055	29.6	12
GGGCTAA	50	9.078571E-6	25.900002	1
TACGCCA	40	0.0019297702	23.125002	5
CTCTAAT	65	2.678149E-6	22.769228	1
AGAGGTC	140	0.0	22.464285	8
GATTTAT	50	2.699341E-4	22.2	1
ATACGGC	60	3.7212267E-5	21.583334	29
TAAACCG	45	0.0038230289	20.555555	5
CTTAACG	55	5.1378796E-4	20.181818	17
GATGCTA	55	5.1378796E-4	20.181818	14
GTCTTGG	55	5.1378796E-4	20.181818	1
TCGCCAT	120	2.382876E-10	20.041668	13
ATTAGCT	120	2.382876E-10	20.041668	3
CTTATAC	740	0.0	19.75	37
TCTAATA	75	9.24975E-6	19.733334	2
TAATGTC	75	9.24975E-6	19.733334	7
ACGGTAC	85	1.2426208E-6	19.588236	3
>>END_MODULE