FastQCFastQC Report
Fri 10 Feb 2017
ERR1631651.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631651.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938905
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.17754724918921508No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA16490.17563012232334474No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA15710.1673225725712399No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA15280.16274276950277183No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15030.16008009330017414No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA14870.1583759805305116No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14130.1504944589708224No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13550.14431705018079571No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC13550.14431705018079571No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12700.1352639510919635No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG11820.12589133085881957No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA11470.12216358417518279No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC11190.11918138682827335No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT10420.1109803441242724No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT10350.11023479478754507No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA10210.10874369611409034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13750.030.5418191
TAGACCG250.005495837429.65
CCGCTTA551.9021774E-523.54545425
GGACGTA551.9021774E-523.54545427
AAGACCG400.001930925123.1250025
CCTAGAC502.7015916E-422.23
ACACGAA603.7253496E-521.58333417
AAACACG603.7253496E-521.58333415
CTTAGGA1750.021.1428592
GACCGTT1059.822543E-1021.1428577
GTATCAA19900.021.010052
CGCAACA450.003825294820.55555526
CGTTATT555.142129E-420.18181810
CTTATAC10900.019.34862337
GCAGTCG2200.019.3409089
CAGTCGG2200.019.34090810
GATATAC1254.129106E-1019.243
TTCTGCG4750.018.69473618
CAATGCA609.235096E-418.59
AACACGA701.2188957E-418.516
Produced by FastQC (version 0.11.5)