##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631651.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	938905
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61760348491062	34.0	31.0	34.0	31.0	34.0
2	32.788174522449026	34.0	31.0	34.0	31.0	34.0
3	32.8747871190376	34.0	31.0	34.0	31.0	34.0
4	36.29733146590976	37.0	37.0	37.0	35.0	37.0
5	36.213627576804896	37.0	35.0	37.0	35.0	37.0
6	36.26837645981223	37.0	37.0	37.0	35.0	37.0
7	36.259625840740014	37.0	37.0	37.0	35.0	37.0
8	36.240360845878975	37.0	37.0	37.0	35.0	37.0
9	38.07458475564621	39.0	38.0	39.0	37.0	39.0
10	38.00355307512475	39.0	38.0	39.0	35.0	39.0
11	38.078109073867964	39.0	38.0	39.0	37.0	39.0
12	38.01315468551132	39.0	38.0	39.0	35.0	39.0
13	38.062169229048735	39.0	38.0	39.0	37.0	39.0
14	39.45604187857131	40.0	39.0	41.0	37.0	41.0
15	39.46350269729099	41.0	39.0	41.0	37.0	41.0
16	39.41540624450823	40.0	39.0	41.0	37.0	41.0
17	39.38789334384203	40.0	39.0	41.0	37.0	41.0
18	39.40906161965268	40.0	39.0	41.0	37.0	41.0
19	39.43928192948168	41.0	39.0	41.0	37.0	41.0
20	39.42878565989104	41.0	39.0	41.0	37.0	41.0
21	39.39077542456372	40.0	39.0	41.0	37.0	41.0
22	39.366175491663164	40.0	39.0	41.0	36.0	41.0
23	39.301603463609204	40.0	39.0	41.0	36.0	41.0
24	39.31934008232995	40.0	39.0	41.0	36.0	41.0
25	39.26295951134566	40.0	39.0	41.0	36.0	41.0
26	39.20428264840426	40.0	39.0	41.0	36.0	41.0
27	39.09697253715765	40.0	39.0	41.0	36.0	41.0
28	39.08804618145606	40.0	39.0	41.0	35.0	41.0
29	39.06453794579856	40.0	39.0	41.0	35.0	41.0
30	39.021564482029596	40.0	39.0	41.0	35.0	41.0
31	38.94366416197592	40.0	38.0	41.0	35.0	41.0
32	38.8910656562698	40.0	38.0	41.0	35.0	41.0
33	38.83595145408747	40.0	38.0	41.0	35.0	41.0
34	38.80778992549832	40.0	38.0	41.0	35.0	41.0
35	38.729106778641075	40.0	38.0	41.0	35.0	41.0
36	38.68714726197006	40.0	38.0	41.0	35.0	41.0
37	38.64855336801913	40.0	38.0	41.0	35.0	41.0
38	38.60928741459466	40.0	38.0	41.0	35.0	41.0
39	38.5371214340109	40.0	38.0	41.0	35.0	41.0
40	38.21856417848451	40.0	38.0	41.0	34.0	41.0
41	38.29569658272136	40.0	38.0	41.0	34.0	41.0
42	38.283065911886716	40.0	37.0	41.0	34.0	41.0
43	37.813484857360436	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	1.0
15	1.0
16	1.0
17	7.0
18	6.0
19	20.0
20	30.0
21	73.0
22	146.0
23	260.0
24	419.0
25	757.0
26	1240.0
27	1933.0
28	2871.0
29	4168.0
30	6101.0
31	8641.0
32	11494.0
33	15723.0
34	23115.0
35	34493.0
36	53792.0
37	97904.0
38	224158.0
39	451549.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.52570813873608	17.05518662697504	13.048178463209803	27.370926771079073
2	19.899350839541807	20.030461015757716	33.798307603005625	26.27188054169485
3	20.352218808079627	20.575031552713	29.987378914799688	29.08537072440769
4	15.02494927601834	16.296643430379003	34.56388026477652	34.11452702882613
5	16.056789558049005	33.74729072696386	34.08353347782789	16.112386237159242
6	33.204424302778236	34.84410030833791	15.846864166236202	16.10461122264766
7	29.67403517927799	28.83550518955592	20.53828662111715	20.95217301004894
8	26.7639431039349	32.0371070555594	20.427519291089087	20.771430549416607
9	27.910917504965894	13.457485049073123	18.275544384149622	40.356053061811366
10	18.568864794627785	24.341120773667196	30.653580500689632	26.436433931015387
11	37.84536241685794	20.408454529478487	19.740868351963194	22.005314701700385
12	22.33921429750614	24.613033267476474	27.543681203103613	25.504071231913773
13	33.71406052795544	18.456606365926266	22.092863495241797	25.7364696108765
14	23.594719380555006	20.50878416879237	24.169537919171802	31.72695853148082
15	28.44718049216907	25.064303630292734	21.077744819763446	25.41077105777475
16	24.891975226460612	24.958542131525554	23.071237239124297	27.078245402889532
17	25.87801747780659	24.62166033837289	22.951736331151714	26.5485858526688
18	25.8399944616335	22.510477630857224	24.46434942832342	27.18517847918586
19	27.354631192719175	23.13418290455371	24.373711930386992	25.137473972340118
20	28.705886112013463	21.815306127883012	23.97707968324804	25.501728076855485
21	26.474989482429002	23.332392521075082	23.097224958861652	27.095393037634263
22	27.207118931095266	23.842880802637115	22.50206357405701	26.447936692210604
23	26.7258135807137	23.231956374713096	23.572565914549397	26.46966413002381
24	26.754570483701762	22.740319840665457	23.83020646391275	26.674903211720036
25	26.85213093976494	23.000516559183303	24.25261341669285	25.89473908435891
26	26.77874758362135	23.55467273046794	23.561382674498486	26.10519701141223
27	26.214792763911156	22.763218856007796	24.749575303145686	26.272413076935365
28	25.578306644442193	23.827969815902566	23.467656472167047	27.12606706748819
29	26.164095409013694	24.33654097059873	23.553501152938797	25.945862467448784
30	25.83807733476763	23.496732896299413	24.614630873198035	26.050558895734927
31	26.778428062477033	23.437621484601742	23.845649985887814	25.938300467033404
32	24.4693552595843	23.343895282270307	24.312470377727248	27.874279080418145
33	24.88814097272887	22.977724050889066	25.62964304162828	26.504491934753784
34	25.407575846331632	22.820945676080115	25.11872873187383	26.65274974571442
35	24.96109830068005	24.000085205638484	25.524094556957305	25.51472193672416
36	25.192005580969322	22.984327487871507	25.95693920045159	25.866727730707577
37	25.726138427210422	22.61123329836352	25.364227477753342	26.298400796672723
38	24.757669838801583	22.14846017435204	26.552100585256227	26.541769401590148
39	24.313535448208285	21.281173281641912	27.46742215666122	26.93786911348858
40	23.975588584574584	22.019160617953894	28.190924534431065	25.814326263040456
41	22.698888598953033	21.308545593004617	29.096660471506702	26.895905336535648
42	21.655439048679046	21.695805219910426	29.726223632848903	26.922532098561625
43	20.91596061369361	22.180305781735107	29.577113765503434	27.32661983906785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	17.0
2	17.0
3	40.5
4	64.0
5	64.0
6	104.5
7	145.0
8	156.5
9	168.0
10	255.5
11	343.0
12	343.0
13	679.0
14	1015.0
15	2080.5
16	3146.0
17	3096.5
18	3047.0
19	3047.0
20	3472.5
21	3898.0
22	3646.5
23	3395.0
24	3862.5
25	4330.0
26	4330.0
27	5031.0
28	5732.0
29	7971.5
30	10211.0
31	11619.0
32	13027.0
33	13027.0
34	15485.0
35	17943.0
36	20134.0
37	22325.0
38	26163.0
39	30001.0
40	30001.0
41	35061.0
42	40121.0
43	44272.0
44	48423.0
45	60220.5
46	72018.0
47	72018.0
48	78070.0
49	84122.0
50	92617.5
51	101113.0
52	102915.5
53	104718.0
54	104718.0
55	93878.5
56	83039.0
57	77515.0
58	71991.0
59	65825.5
60	59660.0
61	59660.0
62	54135.0
63	48610.0
64	40725.5
65	32841.0
66	28480.5
67	24120.0
68	24120.0
69	20655.5
70	17191.0
71	14817.0
72	12443.0
73	10147.5
74	7852.0
75	7852.0
76	6316.5
77	4781.0
78	3912.0
79	3043.0
80	2403.0
81	1763.0
82	1763.0
83	1387.0
84	1011.0
85	820.5
86	630.0
87	491.5
88	353.0
89	353.0
90	269.0
91	185.0
92	113.0
93	41.0
94	26.0
95	11.0
96	11.0
97	6.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	938905.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.5674110158955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.79079749293274	43.019951317295494
2	6.405274162220452	6.349857141365538
3	2.0391318017450284	3.0322345239803807
4	1.0335222561487882	2.0491608985841046
5	0.6729288899513941	1.6677671436345531
6	0.44196326682968456	1.314418494052492
7	0.3093834528325917	1.0734735737648997
8	0.2509879692441869	0.9952659065257243
9	0.20177023579834324	0.9001105387731568
>10	1.5034655285949046	15.10494133649847
>50	0.206846241829101	7.091524252013215
>100	0.13100097416356113	12.042975505177832
>500	0.009480333653430253	3.0788015377169797
>1k	0.0034473940557928195	2.2795178306171504
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1667	0.17754724918921508	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1649	0.17563012232334474	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1571	0.1673225725712399	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1528	0.16274276950277183	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1503	0.16008009330017414	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1487	0.1583759805305116	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1413	0.1504944589708224	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1355	0.14431705018079571	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1355	0.14431705018079571	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1270	0.1352639510919635	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1182	0.12589133085881957	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	1147	0.12216358417518279	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1119	0.11918138682827335	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1042	0.1109803441242724	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1035	0.11023479478754507	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1021	0.10874369611409034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.0650704810390828E-4	0.0	0.0	0.0	0.0
3	1.0650704810390828E-4	0.0	0.0	1.0650704810390828E-4	0.0
4	1.0650704810390828E-4	0.0	0.0	1.0650704810390828E-4	0.0
5	1.0650704810390828E-4	0.0	0.0	1.0650704810390828E-4	0.0
6	1.0650704810390828E-4	0.0	0.0	1.0650704810390828E-4	0.0
7	1.0650704810390828E-4	0.0	0.0	1.0650704810390828E-4	0.0
8	3.195211443117248E-4	0.0	0.0	1.0650704810390828E-4	0.0
9	3.195211443117248E-4	0.0	0.0	2.1301409620781657E-4	0.0
10	3.195211443117248E-4	0.0	0.0	5.325352405195413E-4	0.0
11	3.195211443117248E-4	0.0	0.0	5.325352405195413E-4	0.0
12	3.195211443117248E-4	0.0	0.0	5.325352405195413E-4	0.0
13	3.195211443117248E-4	0.0	0.0	5.325352405195413E-4	0.0
14	4.2602819241563313E-4	0.0	0.0	5.325352405195413E-4	0.0
15	4.2602819241563313E-4	0.0	0.0	6.390422886234496E-4	0.0
16	4.2602819241563313E-4	0.0	0.0	0.0010650704810390827	0.0
17	4.2602819241563313E-4	0.0	0.0	0.0017041127696625325	0.0
18	4.2602819241563313E-4	0.0	0.0	0.0018106198177664408	0.0
19	4.2602819241563313E-4	0.0	0.0	0.002343155058285982	0.0
20	5.325352405195413E-4	0.0	0.0	0.00308870439501334	0.0
21	6.390422886234496E-4	0.0	0.0	0.004792817164675872	0.0
22	6.390422886234496E-4	0.0	0.0	0.008307549752104846	0.0
23	6.390422886234496E-4	0.0	0.0	0.010011662521767379	0.0
24	6.390422886234496E-4	0.0	0.0	0.012780845772468992	0.0
25	6.390422886234496E-4	0.0	0.0	0.013845916253508075	0.0
26	6.390422886234496E-4	0.0	0.0	0.015550029023170608	0.0
27	6.390422886234496E-4	0.0	0.0	0.03131307214254903	0.0
28	6.390422886234496E-4	0.0	0.0	0.1157731612889483	1.0650704810390828E-4
29	6.390422886234496E-4	0.0	0.0	0.2649895356825238	1.0650704810390828E-4
30	6.390422886234496E-4	0.0	0.0	0.4490337148060773	1.0650704810390828E-4
31	7.455493367273579E-4	0.0	0.0	1.0376981696763783	1.0650704810390828E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1375	0.0	30.541819	1
TAGACCG	25	0.0054958374	29.6	5
CCGCTTA	55	1.9021774E-5	23.545454	25
GGACGTA	55	1.9021774E-5	23.545454	27
AAGACCG	40	0.0019309251	23.125002	5
CCTAGAC	50	2.7015916E-4	22.2	3
ACACGAA	60	3.7253496E-5	21.583334	17
AAACACG	60	3.7253496E-5	21.583334	15
CTTAGGA	175	0.0	21.142859	2
GACCGTT	105	9.822543E-10	21.142857	7
GTATCAA	1990	0.0	21.01005	2
CGCAACA	45	0.0038252948	20.555555	26
CGTTATT	55	5.142129E-4	20.181818	10
CTTATAC	1090	0.0	19.348623	37
GCAGTCG	220	0.0	19.340908	9
CAGTCGG	220	0.0	19.340908	10
GATATAC	125	4.129106E-10	19.24	3
TTCTGCG	475	0.0	18.694736	18
CAATGCA	60	9.235096E-4	18.5	9
AACACGA	70	1.2188957E-4	18.5	16
>>END_MODULE