FastQCFastQC Report
Fri 10 Feb 2017
ERR1631650.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631650.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences670003
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA14460.2158199291644963No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA13360.19940209222943775No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA13200.197014043220702No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12830.1914916798880005No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA11680.17432757763771206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11580.17283504700725222No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11470.17119326331374635No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG10770.1607455489005273No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9770.14582024259592868No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9670.1443277119654688No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9440.14089489151541112No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA9420.14059638538931915No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8840.13193970773265196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8680.12955165872391616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8620.12865614034564024No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8550.12761136890431835No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7280.10865622989747808No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT7240.10805921764529412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTTAG200.001841388137.024
GGTATCA10050.027.243781
GAACTAA451.3224076E-424.66666634
ATACGGC1002.0008883E-1124.0529
CCTAGAG400.001930350923.1250023
AACGATT400.001930350923.12500222
GGACGTA652.6800735E-622.7692327
AAACACG652.6800735E-622.7692315
GCGGTAA6050.021.71074323
CAGTCGG2050.020.756110
TAGAGTG450.00382416820.5555555
CGCGGTA6650.020.30827122
AGTCGGT2100.020.26190411
ATTTAGA555.140016E-420.1818182
GCAGTCG2200.020.1818189
AACTAAG555.140016E-420.18181835
ACACGAA759.256313E-619.73333417
GGTAATA6700.019.32835825
TAATACG6150.019.25203127
CGGTAAT6850.018.9051124
Produced by FastQC (version 0.11.5)