FastQCFastQC Report
Fri 10 Feb 2017
ERR1631649.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631649.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences927583
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21010.22650264181210736No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA18740.20203043824649652No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA17470.18833894109745436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17190.18532034330081515No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16210.17475525101257786No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15900.17141323202344158No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA15540.16753217771347684No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG14950.16117156092770135No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT13470.14521611543117974No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC12970.13982576222289542No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12900.1390711127737356No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT12550.13529786552793657No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA12060.13001531938381794No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11620.12527180856052772No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA11550.12451715911136793No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA9380.10112302618741395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTA250.005495802529.625
GGTATCA14400.028.9062521
GTTCTAG1059.822543E-1021.1428591
ACCGAGT450.00382525920.5555578
AAGTAGT555.142065E-420.1818181
GTACTAT555.142065E-420.1818181
TGTCCGA851.2447781E-619.58823612
CTTATAC12950.019.037
GTATCAA22100.018.7511312
TGCGGGT4550.018.70329921
CTGTCCG801.6161228E-518.511
AGACGCG500.007033882718.57
AGTAGTA500.007033882718.52
TATACTG609.234986E-418.55
TTTTACG609.234986E-418.54
GTAACGT4500.018.49999826
GTCGGTG3850.018.25973912
GCAGTCG3750.018.2533329
GGTAACG4650.017.90322525
ATGCTAG1156.401024E-817.69565215
Produced by FastQC (version 0.11.5)