##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631649.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	927583
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.65763602825839	34.0	31.0	34.0	31.0	34.0
2	32.818961753287844	34.0	31.0	34.0	31.0	34.0
3	32.90390940756784	34.0	31.0	34.0	31.0	34.0
4	36.324756922022075	37.0	37.0	37.0	35.0	37.0
5	36.24346069300537	37.0	37.0	37.0	35.0	37.0
6	36.29766392872659	37.0	37.0	37.0	35.0	37.0
7	36.28718292594841	37.0	37.0	37.0	35.0	37.0
8	36.26996182551858	37.0	37.0	37.0	35.0	37.0
9	38.09976034489636	39.0	38.0	39.0	37.0	39.0
10	38.037677490855266	39.0	38.0	39.0	35.0	39.0
11	38.10752676579886	39.0	38.0	39.0	37.0	39.0
12	38.04797306548309	39.0	38.0	39.0	35.0	39.0
13	38.094866982253876	39.0	38.0	39.0	37.0	39.0
14	39.49735171946877	41.0	39.0	41.0	37.0	41.0
15	39.49706710881937	41.0	39.0	41.0	37.0	41.0
16	39.459057572206476	41.0	39.0	41.0	37.0	41.0
17	39.434889384561814	41.0	39.0	41.0	37.0	41.0
18	39.45878805454606	41.0	39.0	41.0	37.0	41.0
19	39.4883412050458	41.0	39.0	41.0	37.0	41.0
20	39.47815774976471	41.0	39.0	41.0	37.0	41.0
21	39.446077601680926	41.0	39.0	41.0	37.0	41.0
22	39.424539906401904	41.0	39.0	41.0	37.0	41.0
23	39.35982763806581	41.0	39.0	41.0	36.0	41.0
24	39.38341905791719	41.0	39.0	41.0	36.0	41.0
25	39.32675243077978	41.0	39.0	41.0	36.0	41.0
26	39.274188940504516	40.0	39.0	41.0	36.0	41.0
27	39.17826329288053	40.0	39.0	41.0	36.0	41.0
28	39.16800329458388	40.0	39.0	41.0	36.0	41.0
29	39.14759218312539	40.0	39.0	41.0	36.0	41.0
30	39.100766184805025	40.0	39.0	41.0	35.0	41.0
31	39.015749534003966	40.0	39.0	41.0	35.0	41.0
32	38.97275176453212	40.0	38.0	41.0	35.0	41.0
33	38.91559677139404	40.0	38.0	41.0	35.0	41.0
34	38.882611043971266	40.0	38.0	41.0	35.0	41.0
35	38.81706003667596	40.0	38.0	41.0	35.0	41.0
36	38.77654937617442	40.0	38.0	41.0	35.0	41.0
37	38.73830805437357	40.0	38.0	41.0	35.0	41.0
38	38.701514581444464	40.0	38.0	41.0	35.0	41.0
39	38.64002035397372	40.0	38.0	41.0	35.0	41.0
40	38.321360999500854	40.0	38.0	41.0	34.0	41.0
41	38.392817677771156	40.0	38.0	41.0	34.0	41.0
42	38.38529921311624	40.0	38.0	41.0	34.0	41.0
43	37.938874472688696	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	1.0
15	2.0
16	0.0
17	2.0
18	4.0
19	13.0
20	30.0
21	49.0
22	113.0
23	220.0
24	393.0
25	683.0
26	1134.0
27	1844.0
28	2828.0
29	4167.0
30	5874.0
31	8110.0
32	11024.0
33	14911.0
34	22092.0
35	32693.0
36	50488.0
37	91525.0
38	210153.0
39	469227.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.00346491904228	17.24449456275072	13.419068697895497	27.332971820311496
2	19.473190000247957	19.866146749131882	34.33568748025783	26.32497577036233
3	20.116151330932112	20.97084573563767	30.271468968275617	28.641533965154604
4	14.887616525960482	16.5574401428228	34.757213101145666	33.79773023007105
5	15.977869365868067	33.70059606525777	34.262055255432664	16.059479313441493
6	32.63869648322576	34.82987506239334	16.141843910464075	16.389584543916826
7	29.391224289362782	29.006029649098785	20.896890089620012	20.705855971918417
8	26.72688050557201	31.984738831996708	20.41757988233937	20.870800780091916
9	27.98121569713977	13.718017686826947	18.721990377141452	39.57877623889183
10	18.706358352837427	24.653319433409195	30.44428369213321	26.196038521620167
11	37.326794475534804	20.77463687885612	19.75952556267202	22.13904308293705
12	22.499118677250447	25.03549547587655	27.11574058601764	25.349645260855365
13	33.24715955337689	18.666685353224455	22.24178321508695	25.8443718783117
14	23.26347076218516	20.93235861373052	24.384987650700797	31.419182973383514
15	28.252027042323974	25.349321839662863	21.487241572991312	24.911409545021847
16	24.61138248544874	25.154191053522972	23.564144664143264	26.67028179688502
17	25.6206722201679	24.94849517509484	23.055510935409554	26.375321669327707
18	25.52720349553625	22.91191192594086	24.646096360110093	26.9147882184128
19	26.918453658594434	23.48447524372482	24.843598901661633	24.753472196019118
20	28.180119730525462	22.199415038869837	24.558988252264218	25.06147697834048
21	26.005435632175235	23.808543278606876	23.501185338670503	26.684835750547393
22	26.982814475901346	24.14932140843461	22.959993876558755	25.907870239105286
23	26.294358564139277	23.605326962654555	24.12204622120069	25.97826825200548
24	26.508679007700657	23.210214072487314	24.282031904422567	25.999075015389455
25	26.142781831922317	23.205254947535693	24.645018289468435	26.006944931073555
26	26.070119870674645	24.12506481899733	24.093477349196785	25.711337961131242
27	25.645252230797677	23.134641320507168	25.248414427603784	25.971692021091375
28	24.935773941523294	24.00141011639929	24.044856363258056	27.01795957881936
29	26.123160946244163	24.46724444065922	24.049923295273846	25.359671317822773
30	25.419288624306397	23.41202889660548	25.037867231288196	26.130815247799926
31	26.21921704041579	23.739223336348335	24.458404261397632	25.583155361838237
32	24.255187945445314	23.697286388387887	24.582274578124004	27.4652510880428
33	24.53775026062358	22.953741066837146	26.324436735041502	26.184071937497777
34	25.09500497529601	22.9695887052695	25.817959147591107	26.117447171843384
35	24.617743102234517	24.192120812908385	25.840275209873404	25.349860874983694
36	24.648360308457573	23.23662680320791	26.23743643425979	25.87757645407473
37	24.846617499458272	22.741361150430745	26.11647690826589	26.2955444418451
38	24.33399490935043	22.005362323371603	27.163283501314705	26.49735926596326
39	23.8427181179474	21.15940029086346	28.22863290940002	26.769248681789122
40	23.560371416897464	22.135701063947916	28.74707708097281	25.55685043818181
41	21.98293845402514	21.413501541101983	29.850374575644445	26.753185429228438
42	20.792317237379297	21.938737557717207	30.33615320677503	26.93279199812847
43	20.09114009204567	22.383441697400663	30.17541287410399	27.35000533644968
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	22.5
2	26.0
3	47.5
4	69.0
5	69.0
6	105.5
7	142.0
8	175.0
9	208.0
10	305.0
11	402.0
12	402.0
13	854.5
14	1307.0
15	2593.5
16	3880.0
17	3812.0
18	3744.0
19	3744.0
20	4073.5
21	4403.0
22	3890.0
23	3377.0
24	3625.0
25	3873.0
26	3873.0
27	4556.5
28	5240.0
29	7038.0
30	8836.0
31	10430.0
32	12024.0
33	12024.0
34	15056.5
35	18089.0
36	20329.5
37	22570.0
38	27556.0
39	32542.0
40	32542.0
41	37682.0
42	42822.0
43	47516.0
44	52210.0
45	64289.0
46	76368.0
47	76368.0
48	83346.5
49	90325.0
50	99185.0
51	108045.0
52	106237.0
53	104429.0
54	104429.0
55	91591.5
56	78754.0
57	72071.5
58	65389.0
59	59700.5
60	54012.0
61	54012.0
62	48552.5
63	43093.0
64	35715.0
65	28337.0
66	24573.0
67	20809.0
68	20809.0
69	17712.0
70	14615.0
71	12465.5
72	10316.0
73	8372.5
74	6429.0
75	6429.0
76	5251.5
77	4074.0
78	3373.0
79	2672.0
80	2200.5
81	1729.0
82	1729.0
83	1404.0
84	1079.0
85	883.0
86	687.0
87	535.0
88	383.0
89	383.0
90	282.0
91	181.0
92	122.0
93	63.0
94	36.0
95	9.0
96	9.0
97	5.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	927583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.01722053087579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.78300749699758	41.190615887851045
2	6.7961920782308765	6.526685075812061
3	2.160794476062142	3.1126603463692217
4	1.1693555366673392	2.2459681073262487
5	0.6997278086361812	1.6799492249434982
6	0.5038347001074411	1.4515645143699998
7	0.3680278650618247	1.2370172610726724
8	0.28457945205370366	1.0931771446254785
9	0.21512658762049697	0.9296802719845358
>10	1.655701929133102	16.071411922309665
>50	0.21237534044362844	7.107289578288764
>100	0.13932789644489796	12.340923614660596
>500	0.008567087482069217	2.5867452323351845
>1k	0.0033817450587115333	2.4263118180509933
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2101	0.22650264181210736	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1874	0.20203043824649652	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1747	0.18833894109745436	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1719	0.18532034330081515	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1621	0.17475525101257786	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1590	0.17141323202344158	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1554	0.16753217771347684	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1495	0.16117156092770135	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1347	0.14521611543117974	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1297	0.13982576222289542	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1290	0.1390711127737356	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1255	0.13529786552793657	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1206	0.13001531938381794	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1162	0.12527180856052772	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1155	0.12451715911136793	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	938	0.10112302618741395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0780706416568652E-4	0.0	0.0	0.0	0.0
9	2.1561412833137305E-4	0.0	0.0	0.0	0.0
10	2.1561412833137305E-4	0.0	0.0	0.0	0.0
11	2.1561412833137305E-4	0.0	0.0	0.0	0.0
12	2.1561412833137305E-4	0.0	0.0	0.0	0.0
13	2.1561412833137305E-4	0.0	0.0	0.0	0.0
14	2.1561412833137305E-4	0.0	0.0	0.0	0.0
15	2.1561412833137305E-4	0.0	0.0	5.390353208284326E-4	0.0
16	2.1561412833137305E-4	0.0	0.0	0.0010780706416568652	0.0
17	2.1561412833137305E-4	0.0	0.0	0.002048334219148044	0.0
18	3.234211924970596E-4	0.0	0.0	0.0021561412833137304	0.0
19	3.234211924970596E-4	0.0	0.0	0.0023717554116451036	0.0
20	4.312282566627461E-4	0.0	0.0	0.003665440181633342	0.0
21	6.468423849941192E-4	0.0	0.0	0.006145002657444131	1.0780706416568652E-4
22	6.468423849941192E-4	0.0	0.0	0.008732372197420608	1.0780706416568652E-4
23	6.468423849941192E-4	0.0	0.0	0.01056509228823728	1.0780706416568652E-4
24	6.468423849941192E-4	0.0	0.0	0.013475883020710814	1.0780706416568652E-4
25	6.468423849941192E-4	0.0	0.0	0.015416410175693172	1.0780706416568652E-4
26	6.468423849941192E-4	0.0	0.0	0.018758429164829456	1.0780706416568652E-4
27	6.468423849941192E-4	0.0	0.0	0.04301501860210892	1.0780706416568652E-4
28	6.468423849941192E-4	0.0	0.0	0.15793734900273076	1.0780706416568652E-4
29	6.468423849941192E-4	0.0	0.0	0.3446591841376998	1.0780706416568652E-4
30	6.468423849941192E-4	0.0	0.0	0.5816191111738788	1.0780706416568652E-4
31	7.546494491598056E-4	0.0	0.0	1.2840899412774922	1.0780706416568652E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCTA	25	0.0054958025	29.6	25
GGTATCA	1440	0.0	28.906252	1
GTTCTAG	105	9.822543E-10	21.142859	1
ACCGAGT	45	0.003825259	20.555557	8
AAGTAGT	55	5.142065E-4	20.181818	1
GTACTAT	55	5.142065E-4	20.181818	1
TGTCCGA	85	1.2447781E-6	19.588236	12
CTTATAC	1295	0.0	19.0	37
GTATCAA	2210	0.0	18.751131	2
TGCGGGT	455	0.0	18.703299	21
CTGTCCG	80	1.6161228E-5	18.5	11
AGACGCG	50	0.0070338827	18.5	7
AGTAGTA	50	0.0070338827	18.5	2
TATACTG	60	9.234986E-4	18.5	5
TTTTACG	60	9.234986E-4	18.5	4
GTAACGT	450	0.0	18.499998	26
GTCGGTG	385	0.0	18.259739	12
GCAGTCG	375	0.0	18.253332	9
GGTAACG	465	0.0	17.903225	25
ATGCTAG	115	6.401024E-8	17.695652	15
>>END_MODULE