FastQCFastQC Report
Fri 10 Feb 2017
ERR1631646.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631646.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480594
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA12480.25967864767350407No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA12370.2573898134392023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10900.2268026650353521No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC10730.2232653757641585No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA10660.22180884488778468No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9910.2062031569266366No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9660.20100126093958726No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG9610.19996088174217738No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT9440.19642359247098382No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9280.19309437903927223No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC9110.18955708976807867No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8350.17374332596744863No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA8330.17332717428848468No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT8310.1729110226095207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8290.17249487093055677No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC6840.14232387420567047No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6310.13129585471312585No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA6100.12692626208400437No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA5810.12089206273902713No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5580.11610631843094171No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA5570.11589824259145974No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5560.11569016675197777No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG5520.11485786339404985No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5490.11423363587560395No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT5390.11215287748078419No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5270.10965596740700051No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5040.10487022309891508No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA4970.10341369222254128No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4880.1015410096672035No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4870.10133293382772153No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA4830.10050063046979363No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4810.10008447879082968No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG200.001840870237.01
TCTTAAC200.001840870237.03
GGTATCA7550.030.8741741
GTATGCC250.005493229.632
TATACTG250.005493229.65
TTTTACG701.918088E-723.7857134
GTCTCAC400.001929560723.1250021
ATTGGTC400.001929560723.12500217
TAACACC753.731402E-722.235
TTACGAG806.9427915E-720.81256
CTAAGTC450.003822617520.55555534
TAAGTCT450.003822617520.55555535
GATTAGA450.003822617520.5555552
TAGTATA450.003822617520.5555552
AACGATT555.13711E-420.18181822
CCAACCG851.2422242E-619.58823414
GTATCAA12100.019.111572
CAGTCGG1551.8189894E-1219.09677510
CTCACTC500.007029070518.53
TTTACGA902.1469168E-618.55
Produced by FastQC (version 0.11.5)