##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631646.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480594
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6349226165953	34.0	31.0	34.0	31.0	34.0
2	32.79521592029863	34.0	31.0	34.0	31.0	34.0
3	32.8815882012676	34.0	31.0	34.0	31.0	34.0
4	36.307592271231016	37.0	37.0	37.0	35.0	37.0
5	36.22258288701066	37.0	35.0	37.0	35.0	37.0
6	36.27123101828154	37.0	37.0	37.0	35.0	37.0
7	36.26545899449431	37.0	37.0	37.0	35.0	37.0
8	36.24800351232017	37.0	37.0	37.0	35.0	37.0
9	38.075708394195516	39.0	38.0	39.0	37.0	39.0
10	38.022303649234075	39.0	38.0	39.0	35.0	39.0
11	38.08884422194201	39.0	38.0	39.0	37.0	39.0
12	38.02297157267881	39.0	38.0	39.0	35.0	39.0
13	38.08164479789594	39.0	38.0	39.0	37.0	39.0
14	39.461002426164285	40.0	39.0	41.0	37.0	41.0
15	39.47462307061678	41.0	39.0	41.0	37.0	41.0
16	39.42687174621406	40.0	39.0	41.0	37.0	41.0
17	39.4060495969571	40.0	39.0	41.0	37.0	41.0
18	39.43294964148533	41.0	39.0	41.0	37.0	41.0
19	39.44457275787879	41.0	39.0	41.0	37.0	41.0
20	39.45095236311731	41.0	39.0	41.0	37.0	41.0
21	39.416800875583135	41.0	39.0	41.0	37.0	41.0
22	39.39306774533182	40.0	39.0	41.0	37.0	41.0
23	39.33680819985268	40.0	39.0	41.0	36.0	41.0
24	39.34263848487497	41.0	39.0	41.0	36.0	41.0
25	39.28635813181188	40.0	39.0	41.0	36.0	41.0
26	39.21996529294997	40.0	39.0	41.0	36.0	41.0
27	39.11710716321885	40.0	39.0	41.0	36.0	41.0
28	39.11167222229158	40.0	39.0	41.0	35.0	41.0
29	39.091763525969945	40.0	39.0	41.0	35.0	41.0
30	39.05320083063875	40.0	39.0	41.0	35.0	41.0
31	38.96146643528633	40.0	38.0	41.0	35.0	41.0
32	38.91837600968801	40.0	38.0	41.0	35.0	41.0
33	38.851945717175	40.0	38.0	41.0	35.0	41.0
34	38.828797280032624	40.0	38.0	41.0	35.0	41.0
35	38.7481512461662	40.0	38.0	41.0	35.0	41.0
36	38.69982355168812	40.0	38.0	41.0	35.0	41.0
37	38.65583215770484	40.0	38.0	41.0	35.0	41.0
38	38.62504317573669	40.0	38.0	41.0	35.0	41.0
39	38.55343803709576	40.0	38.0	41.0	35.0	41.0
40	38.22011926907119	40.0	38.0	41.0	34.0	41.0
41	38.29663915904069	40.0	38.0	41.0	34.0	41.0
42	38.28018036013766	40.0	37.0	41.0	34.0	41.0
43	37.81075918550793	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	1.0
14	2.0
15	3.0
16	3.0
17	3.0
18	0.0
19	3.0
20	10.0
21	33.0
22	52.0
23	98.0
24	180.0
25	313.0
26	580.0
27	911.0
28	1440.0
29	2169.0
30	3185.0
31	4339.0
32	5978.0
33	8145.0
34	11823.0
35	17574.0
36	26914.0
37	50024.0
38	113285.0
39	233524.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.09063367416156	16.2253378111254	13.043858225446012	27.64017028926703
2	20.681698065310844	19.31817708918547	31.916961093979534	28.083163751524154
3	21.367724108082914	19.49878691785582	29.226748565317084	29.90674040874418
4	15.501025813888647	15.856419347723858	33.816069280931515	34.82648555745598
5	16.94507213989355	32.662496826843444	33.63254639050841	16.759884642754592
6	34.040375035893085	33.25197568009588	15.770067874338839	16.937581409672198
7	30.347653112606483	27.594601680420478	20.297798141466604	21.759947065506434
8	28.363441907306374	29.437529390712324	20.434087816327295	21.764940885654003
9	28.719459668660036	12.982892004477792	17.982954427229636	40.31469389963254
10	19.98651668560157	23.567918034765313	28.94543003033746	27.500135249295667
11	38.74101632563037	19.88996949608193	18.45029276270615	22.91872141558155
12	23.752689380225306	24.01486493797259	25.822419755552506	26.4100259262496
13	35.68042880269001	17.527684490443075	20.925354873344236	25.866531833522682
14	24.09143684690196	20.479864501013328	23.622017753030626	31.806680899054086
15	29.848271097849743	24.461811841179873	20.129256711486203	25.56066034948418
16	25.02195200106535	24.263931717832516	22.776813693054844	27.93730258804729
17	26.9872283049726	23.675701319616973	21.829652471732896	27.507417903677535
18	26.96954185861663	21.12510767924693	23.477196968751173	28.428153493385267
19	28.675763742368822	22.035647552820052	23.666545982679768	25.62204272213136
20	30.64249657715244	20.281568225987005	22.564160185104267	26.511775011756285
21	27.61769809860298	21.970311739222712	22.41892324914585	27.993066913028457
22	28.755872940569375	22.50340203997553	21.852124662396953	26.88860035705814
23	28.127275829494337	21.73393758557119	22.38417458395236	27.75461200098212
24	27.352817554942426	21.351286116763838	24.139502365822295	27.15639396247144
25	27.554859195079423	22.06082472939737	23.513610240660515	26.87070583486269
26	27.517197468133187	23.004448661448123	22.924131387408085	26.55422248301061
27	26.651601975888173	21.456572491541717	24.86277398386164	27.029051548708473
28	26.122048964406545	23.058548379713436	22.238313420475496	28.581089235404523
29	27.465386584102173	23.48239886473822	22.609520718111337	26.44269383304827
30	27.385277385901613	21.708968484833353	23.176319304860236	27.729434824404798
31	27.156185886631963	22.713558637852323	23.486976533206825	26.64327894230889
32	25.314714707216485	22.169024165927997	23.197959192166362	29.318301934689156
33	25.171974681331854	22.219378519082635	25.466401994198844	27.142244805386667
34	26.714024727732767	21.220614489569158	24.27516781316454	27.790192969533535
35	25.217959441857367	22.949724715664367	25.131816044311833	26.700499798166437
36	25.489706488220826	22.34963399459835	25.811807887738926	26.3488516294419
37	25.878392156373152	21.75495324535887	25.607893565046584	26.75876103322139
38	25.594576711319743	21.270552691044834	25.505936403700417	27.628934193935006
39	24.872553548317292	19.91202553506702	27.38174009663042	27.83368081998527
40	24.35506893552562	21.136343774578958	27.725897535133605	26.78268975476182
41	22.399364120234544	20.477159515100062	29.08817005622209	28.035306308443303
42	21.36730795640395	20.775540268917215	30.06134075747929	27.79581101719955
43	21.01857284943216	21.738515254039793	29.40923107654278	27.83368081998527
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	10.0
2	8.0
3	27.0
4	46.0
5	46.0
6	60.5
7	75.0
8	87.0
9	99.0
10	151.5
11	204.0
12	204.0
13	409.5
14	615.0
15	1234.0
16	1853.0
17	1775.5
18	1698.0
19	1698.0
20	1892.5
21	2087.0
22	1682.0
23	1277.0
24	1287.0
25	1297.0
26	1297.0
27	1404.5
28	1512.0
29	1891.5
30	2271.0
31	2686.0
32	3101.0
33	3101.0
34	4047.0
35	4993.0
36	5643.5
37	6294.0
38	7692.0
39	9090.0
40	9090.0
41	11196.5
42	13303.0
43	16309.0
44	19315.0
45	28504.0
46	37693.0
47	37693.0
48	42447.5
49	47202.0
50	52089.0
51	56976.0
52	56186.0
53	55396.0
54	55396.0
55	49055.5
56	42715.0
57	40848.0
58	38981.0
59	37454.5
60	35928.0
61	35928.0
62	32924.0
63	29920.0
64	25923.0
65	21926.0
66	19068.5
67	16211.0
68	16211.0
69	14508.0
70	12805.0
71	10322.0
72	7839.0
73	5497.0
74	3155.0
75	3155.0
76	2506.5
77	1858.0
78	1514.5
79	1171.0
80	951.0
81	731.0
82	731.0
83	607.5
84	484.0
85	360.0
86	236.0
87	177.5
88	119.0
89	119.0
90	95.0
91	71.0
92	44.5
93	18.0
94	10.5
95	3.0
96	3.0
97	2.5
98	2.0
99	3.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	480594.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.75419021142339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4338699919021	38.23523668115604
2	6.507445465458823	5.8247090430321755
3	2.3060475286651707	3.0961586920339164
4	1.223863529528221	2.1909208477332
5	0.7683411503371573	1.7193242994726496
6	0.5083188353936461	1.3649638708353873
7	0.4318041236943185	1.3527530720124765
8	0.31866647483851657	1.1409328023141387
9	0.25634798879134374	1.0325381985615267
>10	1.812738989622649	16.234458542694245
>50	0.23313078900484502	7.329275457423247
>100	0.18678551167252763	15.955206027499159
>500	0.01029895051828473	3.3263844277933705
>1k	0.0023406705723374387	1.197138037438516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	1248	0.25967864767350407	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	1237	0.2573898134392023	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1090	0.2268026650353521	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1073	0.2232653757641585	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1066	0.22180884488778468	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	991	0.2062031569266366	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	966	0.20100126093958726	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	961	0.19996088174217738	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	944	0.19642359247098382	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	928	0.19309437903927223	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	911	0.18955708976807867	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	835	0.17374332596744863	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	833	0.17332717428848468	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	831	0.1729110226095207	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	829	0.17249487093055677	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	684	0.14232387420567047	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	631	0.13129585471312585	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	610	0.12692626208400437	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	581	0.12089206273902713	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	558	0.11610631843094171	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	557	0.11589824259145974	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	556	0.11569016675197777	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	552	0.11485786339404985	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	549	0.11423363587560395	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	539	0.11215287748078419	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	527	0.10965596740700051	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	504	0.10487022309891508	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	497	0.10341369222254128	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	488	0.1015410096672035	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	487	0.10133293382772153	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	483	0.10050063046979363	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	481	0.10008447879082968	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.080758394819744E-4	0.0
4	0.0	0.0	0.0	2.080758394819744E-4	0.0
5	0.0	0.0	0.0	2.080758394819744E-4	0.0
6	0.0	0.0	0.0	2.080758394819744E-4	0.0
7	0.0	0.0	0.0	2.080758394819744E-4	0.0
8	6.242275184459232E-4	0.0	0.0	2.080758394819744E-4	0.0
9	6.242275184459232E-4	0.0	0.0	2.080758394819744E-4	0.0
10	6.242275184459232E-4	0.0	0.0	2.080758394819744E-4	0.0
11	8.323033579278976E-4	0.0	0.0	2.080758394819744E-4	0.0
12	8.323033579278976E-4	0.0	0.0	2.080758394819744E-4	0.0
13	8.323033579278976E-4	0.0	0.0	2.080758394819744E-4	0.0
14	8.323033579278976E-4	0.0	0.0	6.242275184459232E-4	0.0
15	0.001040379197409872	0.0	0.0	8.323033579278976E-4	0.0
16	0.001040379197409872	0.0	0.0	0.0014565308763738207	0.0
17	0.0012484550368918464	0.0	0.0	0.002080758394819744	0.0
18	0.0012484550368918464	0.0	0.0	0.0029130617527476413	0.0
19	0.0012484550368918464	0.0	0.0	0.0035372892711935645	0.0
20	0.0012484550368918464	0.0	0.0	0.0045776684686034365	0.0
21	0.0012484550368918464	0.0	0.0	0.006450351023941206	0.0
22	0.0014565308763738207	0.0	0.0	0.008739185258242924	0.0
23	0.0014565308763738207	2.080758394819744E-4	0.0	0.011028019492544642	0.0
24	0.0014565308763738207	2.080758394819744E-4	2.080758394819744E-4	0.016437991319075976	0.0
25	0.0014565308763738207	2.080758394819744E-4	2.080758394819744E-4	0.017478370516485847	0.0
26	0.0014565308763738207	2.080758394819744E-4	2.080758394819744E-4	0.019767204750787568	0.0
27	0.0018726825553377696	2.080758394819744E-4	2.080758394819744E-4	0.041615167896394877	0.0
28	0.002080758394819744	2.080758394819744E-4	2.080758394819744E-4	0.15418419705614303	0.0
29	0.002080758394819744	2.080758394819744E-4	2.080758394819744E-4	0.35248047208246464	0.0
30	0.0022888342343017182	2.080758394819744E-4	2.080758394819744E-4	0.593016142523627	0.0
31	0.0022888342343017182	2.080758394819744E-4	2.080758394819744E-4	1.340008406263915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCTAG	20	0.0018408702	37.0	1
TCTTAAC	20	0.0018408702	37.0	3
GGTATCA	755	0.0	30.874174	1
GTATGCC	25	0.0054932	29.6	32
TATACTG	25	0.0054932	29.6	5
TTTTACG	70	1.918088E-7	23.785713	4
GTCTCAC	40	0.0019295607	23.125002	1
ATTGGTC	40	0.0019295607	23.125002	17
TAACACC	75	3.731402E-7	22.2	35
TTACGAG	80	6.9427915E-7	20.8125	6
CTAAGTC	45	0.0038226175	20.555555	34
TAAGTCT	45	0.0038226175	20.555555	35
GATTAGA	45	0.0038226175	20.555555	2
TAGTATA	45	0.0038226175	20.555555	2
AACGATT	55	5.13711E-4	20.181818	22
CCAACCG	85	1.2422242E-6	19.588234	14
GTATCAA	1210	0.0	19.11157	2
CAGTCGG	155	1.8189894E-12	19.096775	10
CTCACTC	50	0.0070290705	18.5	3
TTTACGA	90	2.1469168E-6	18.5	5
>>END_MODULE