FastQCFastQC Report
Fri 10 Feb 2017
ERR1631645.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631645.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196280
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9350.47636030160994497No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7950.40503362543305477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7590.38669248013042595No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6870.3500101895251681No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6560.334216425514571No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA5790.29498675361728144No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5320.2710413694721826No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5290.2695129406969635No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4940.25168127165274096No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4780.24352965151823924No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4760.24251069900142652No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4720.24047279396780108No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4710.23996331770939475No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4660.23741593641736294No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4380.22315060118198493No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4280.21805583859792135No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA4160.21194212349704505No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4100.2088852659466069No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4030.20531893213776237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3920.19971469329529246No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3810.1941104544528225No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3790.19309150193600977No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3700.18850621561035255No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA3690.1879967393519462No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3610.18392092928469533No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3590.1829019767678826No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC3500.1783166904422254No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA3350.17067454656613001No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3320.16914611779091096No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3310.16863664153250457No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3240.16507030772366008No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3240.16507030772366008No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3140.1599755451395965No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3070.15640921133075197No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT2950.15029549622987567No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2930.14927654371306298No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2910.14825759119625026No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2880.14672916242103118No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT2870.14621968616262482No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2830.1441817811289994No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2810.14316282861218668No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA2810.14316282861218668No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2800.14265335235378032No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG2740.13959649480334216No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC2700.13755858976971674No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTT2690.13704911351131038No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2670.13603016099449766No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC2660.1355206847360913No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2640.13450173221927858No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCCTGT2590.1319543509272468No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2570.13093539841043406No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG2550.12991644589362134No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2460.12533115956796412No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC2440.12431220705115141No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC2410.12278377827593234No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT2390.12176482575911962No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTCTGT2390.12176482575911962No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2390.12176482575911962No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA2360.12023639698390054No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2360.12023639698390054No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC2350.1197269207254942No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC2340.11921744446708783No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT2330.11870796820868147No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2320.11819849195027511No Hit
CACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCA2290.11667006317505604No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2280.11616058691664968No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA2280.11616058691664968No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2250.1146321581414306No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG2250.1146321581414306No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2250.1146321581414306No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT2240.11412268188302425No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTT2200.11208477684939881No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2190.11157530059099247No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2190.11157530059099247No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2180.1110658243325861No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC2160.11004687181577338No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA2140.10902791929896068No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2140.10902791929896068No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2140.10902791929896068No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2070.10546158549011617No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2070.10546158549011617No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2060.1049521092317098No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG2050.10444263297330345No Hit
GACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTAATTCC2040.10393315671489708No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2040.10393315671489708No Hit
CATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTC2040.10393315671489708No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCT200.001838219637.035
GGTATCA4600.032.5760841
GTATTAG352.3783017E-531.7142871
TATTAGC352.3783017E-531.7142872
ATACACA352.3783017E-531.71428737
ATACGGC551.8391802E-830.27272829
CTAAGTC250.005485380529.634
GTTCAAC250.005485380529.61
ATTGCTG250.005485380529.633
GTTTCCA405.9154434E-527.7513
TACTGCT405.9154434E-527.7533
ACCGTTT405.9154434E-527.7510
AGCTCTG405.9154434E-527.7535
TTGATCA358.843101E-426.4285725
AGCGTGC358.843101E-426.4285728
TACGGCT659.309042E-825.61538530
CTACCGT451.3177577E-424.6666668
CGTTTCC451.3177577E-424.66666612
TGATCAT400.001925516823.1256
AGTTTGA400.001925516823.1252
Produced by FastQC (version 0.11.5)