FastQCFastQC Report
Fri 10 Feb 2017
ERR1631644.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631644.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences726589
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23190.3191625526948523No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22290.30677590770022667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18490.2544767399451409No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA15730.21649102862828917No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA14610.2010765370794218No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA13210.18180842264333757No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13040.17946872303324163No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG12500.17203673603646627No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12360.17010992459285787No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11770.16198979065193664No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC11610.15978772043066988No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT11510.15841142654237814No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC10380.1428593056046816No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT9660.13294998960898113No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA9520.13102317816537273No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG8040.1106540286186551No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA7710.1061122587872924No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7500.10322204162187976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA7430.10225863590007556No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA7320.10074471262295466No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7280.10019419506763795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10850.029.3271881
CTAATGG405.9371683E-527.753
AAACACG601.3362751E-624.66666815
ATTAGAG551.9014105E-523.5454543
GGTCTAT400.001930507123.1251
ATACGGC903.8216967E-922.6111129
ACACGAA603.723857E-521.58333417
GTATTAG1301.8189894E-1221.3461531
TCGTTGG450.00382447420.5555552
TAACCCT759.258079E-619.73333435
GCTCATA759.258079E-619.73333434
TTGGCTA951.6739978E-719.47368422
TTCTGCG3950.019.20253418
TACCCTC902.15E-618.55
TACGAGG902.15E-618.57
TATTAGA701.21841396E-418.52
TTTTACG902.15E-618.54
TTGTACT500.007032453518.4999984
TGCGGGT4000.018.49999821
TGCTATA500.007032453518.4999982
Produced by FastQC (version 0.11.5)