FastQCFastQC Report
Fri 10 Feb 2017
ERR1631643.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631643.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342273
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA5620.16419641631095647No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5380.15718446970692984No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5300.1548471541722543No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5120.14958819421923436No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC4740.13848594542952555No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4510.13176616326733337No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4470.13059750549999563No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT4390.1282601899653201No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4270.12475421666330677No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG4220.12329339445413456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.12183257224496234No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3810.11131465233892243No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3680.10751651459507468No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3660.10693218571140581No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3550.10371837685122694No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3510.10254971908388918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC352.383446E-531.71428519
CGCCGTT352.383446E-531.71428525
CACTATA250.00549101829.62
GGTATCA3450.028.956521
TAGAACA453.997453E-628.7777774
TTTACCG405.928133E-527.7530
GACCGTT405.928133E-527.757
GGACCGT509.066753E-625.9000026
CCGTTTA451.3205613E-424.66666627
GCGGTAA2400.023.12500223
GTTTAAC400.001928432323.12533
ATACGGC400.001928432323.12529
TATACTA400.001928432323.1255
AGGACCG652.6737162E-622.769235
GTTACGG502.6967327E-422.217
TAGGACC753.7249083E-722.24
TTAGGAC855.1539246E-821.7647063
CGCGGTA2650.021.6415122
CTTATAC4600.021.31521837
ACTTAGA1650.021.3030321
Produced by FastQC (version 0.11.5)