##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631643.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	342273
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63393840589237	34.0	31.0	34.0	31.0	34.0
2	32.802117023545534	34.0	31.0	34.0	31.0	34.0
3	32.89988401071659	34.0	31.0	34.0	31.0	34.0
4	36.308122463647436	37.0	37.0	37.0	35.0	37.0
5	36.22500167994554	37.0	35.0	37.0	35.0	37.0
6	36.27761173098667	37.0	37.0	37.0	35.0	37.0
7	36.267114262591555	37.0	37.0	37.0	35.0	37.0
8	36.246662751663145	37.0	37.0	37.0	35.0	37.0
9	38.0776105623289	39.0	38.0	39.0	37.0	39.0
10	38.006924297271475	39.0	38.0	39.0	35.0	39.0
11	38.08593432727677	39.0	38.0	39.0	37.0	39.0
12	38.026405822253	39.0	38.0	39.0	35.0	39.0
13	38.07763393548425	39.0	38.0	39.0	37.0	39.0
14	39.475979700414584	41.0	39.0	41.0	37.0	41.0
15	39.481162697612724	41.0	39.0	41.0	37.0	41.0
16	39.4346209020285	41.0	39.0	41.0	37.0	41.0
17	39.40125864441542	40.0	39.0	41.0	37.0	41.0
18	39.41974096700587	41.0	39.0	41.0	37.0	41.0
19	39.43624241468068	41.0	39.0	41.0	37.0	41.0
20	39.42882728114692	41.0	39.0	41.0	37.0	41.0
21	39.39167565072325	40.0	39.0	41.0	37.0	41.0
22	39.37258562609379	40.0	39.0	41.0	36.0	41.0
23	39.31108793273206	40.0	39.0	41.0	36.0	41.0
24	39.31546747771516	41.0	39.0	41.0	36.0	41.0
25	39.265878407002596	40.0	39.0	41.0	36.0	41.0
26	39.2039395453337	40.0	39.0	41.0	36.0	41.0
27	39.104904564485075	40.0	39.0	41.0	36.0	41.0
28	39.09236486664154	40.0	39.0	41.0	35.0	41.0
29	39.07134947834039	40.0	39.0	41.0	35.0	41.0
30	39.028120827526564	40.0	39.0	41.0	35.0	41.0
31	38.945420760620905	40.0	38.0	41.0	35.0	41.0
32	38.8950282376933	40.0	38.0	41.0	35.0	41.0
33	38.845012606895665	40.0	38.0	41.0	35.0	41.0
34	38.8065082551063	40.0	38.0	41.0	35.0	41.0
35	38.74060472196171	40.0	38.0	41.0	35.0	41.0
36	38.6914538979119	40.0	38.0	41.0	35.0	41.0
37	38.64620346916058	40.0	38.0	41.0	35.0	41.0
38	38.61613098316256	40.0	38.0	41.0	35.0	41.0
39	38.54966941593406	40.0	38.0	41.0	35.0	41.0
40	38.221822930818384	40.0	38.0	41.0	34.0	41.0
41	38.3084672176888	40.0	38.0	41.0	34.0	41.0
42	38.302144779167506	40.0	37.0	41.0	34.0	41.0
43	37.83265697265049	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	1.0
18	0.0
19	6.0
20	13.0
21	33.0
22	40.0
23	103.0
24	174.0
25	272.0
26	446.0
27	689.0
28	1074.0
29	1551.0
30	2229.0
31	2992.0
32	4170.0
33	5629.0
34	8393.0
35	12692.0
36	19721.0
37	35272.0
38	80690.0
39	166082.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.799169084327424	16.426069248816002	12.695421491032013	28.079340175824562
2	20.384313106789026	19.30797930307094	32.68093013471702	27.626777455423014
3	20.89969118218498	19.903994764413202	29.164146748355847	30.03216730504597
4	15.67140849555764	15.821581018660543	33.36108895530761	35.14592153047421
5	17.062695567573254	32.86470156863089	33.17176639699888	16.900836466796974
6	34.72432824090711	33.125604415189045	15.56622929649724	16.5838380474066
7	30.947810665755114	27.731956654483408	19.99252059028904	21.32771208947244
8	27.70186371697446	30.770759014003442	20.076371785095525	21.451005483926576
9	28.53979133615564	13.140972264829538	17.70633383293453	40.612902566080294
10	19.16978552208325	24.07055186941418	30.24661600535245	26.513046603150116
11	38.63553362374479	19.551644447560864	19.254221045773402	22.558600882920942
12	23.099397264756497	23.670870912984665	26.76021772094205	26.469514101316786
13	34.1414601794474	17.676240895425583	21.695839286183833	26.48645963894318
14	24.28558489860433	19.823941707350563	23.27528025874083	32.615193135304274
15	28.82991062689724	24.466726852541683	20.36473808918612	26.338624431374956
16	25.831426960350363	24.0571123050898	22.640406926634586	27.471053807925255
17	26.604494073444297	23.889994244360498	22.427711212979112	27.077800469216097
18	26.726910974572927	21.9847899191581	23.597829802526054	27.69046930374292
19	27.867813119936425	22.602717713637944	23.65597052645111	25.873498639974525
20	29.03939253169254	21.441948386229708	23.148773055426517	26.36988602665124
21	27.102342282330188	22.781230187598787	22.586648669337052	27.529778860733977
22	27.792726858384974	23.05235878962115	22.000274634575323	27.15463971741855
23	27.602527806750754	22.573793433896334	22.678972632956736	27.14470612639618
24	27.344838769052775	22.24072597020507	23.154908508705038	27.259526752037118
25	27.39801269746664	22.164763215328115	23.520114060998093	26.91711002620715
26	27.36412162221385	23.029569963158064	23.07543978052607	26.530868634102017
27	26.915064875114307	22.337140236010438	24.017962269883984	26.729832618991274
28	26.130311184347	23.139423793287815	22.774510405436597	27.95575461692859
29	27.065237398217214	23.526249514276614	22.83469628045449	26.57381680705168
30	26.50837197208077	22.649464024331458	23.949303626052888	26.892860377534895
31	27.282023414058372	22.715785352627872	23.26943696990414	26.732754263409618
32	25.56672597604836	22.646250215471277	23.520406225439928	28.26661758304044
33	25.834640769210544	22.292146911967933	24.76824055651483	27.104971762306697
34	26.04499916733134	22.256795014505965	24.336129347041688	27.362076471121004
35	25.75137390328773	23.27265077876432	24.962822074776568	26.013153243171388
36	25.80571648946893	22.17761845076883	25.29296789404949	26.72369716571275
37	26.174135850622164	21.870845786842665	25.167045019618843	26.787973342916327
38	25.353153769067383	21.219611245993693	25.913233004064008	27.514001980874912
39	24.97421648800811	20.60811106923421	27.038066105126607	27.379606337631074
40	24.537722811907454	21.556476847428808	27.508158692038226	26.397641648625513
41	23.310047827319128	20.59291851825882	28.63182313533349	27.46521051908856
42	22.089676953776664	21.13050109123419	29.091105637897236	27.68871631709191
43	21.611111598051846	21.53164286987288	29.11038849105831	27.746857041016966
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	6.5
2	6.0
3	8.0
4	10.0
5	10.0
6	21.5
7	33.0
8	33.5
9	34.0
10	65.0
11	96.0
12	96.0
13	186.5
14	277.0
15	510.5
16	744.0
17	734.0
18	724.0
19	724.0
20	880.5
21	1037.0
22	921.0
23	805.0
24	913.5
25	1022.0
26	1022.0
27	1212.0
28	1402.0
29	1804.0
30	2206.0
31	2664.5
32	3123.0
33	3123.0
34	4047.0
35	4971.0
36	5778.0
37	6585.0
38	8003.0
39	9421.0
40	9421.0
41	11093.5
42	12766.0
43	14684.5
44	16603.0
45	21109.5
46	25616.0
47	25616.0
48	27979.5
49	30343.0
50	33551.5
51	36760.0
52	37310.0
53	37860.0
54	37860.0
55	34654.0
56	31448.0
57	29567.0
58	27686.0
59	25543.5
60	23401.0
61	23401.0
62	21437.0
63	19473.0
64	16767.5
65	14062.0
66	12587.0
67	11112.0
68	11112.0
69	9514.0
70	7916.0
71	6929.5
72	5943.0
73	4731.0
74	3519.0
75	3519.0
76	2810.0
77	2101.0
78	1748.0
79	1395.0
80	1117.5
81	840.0
82	840.0
83	655.0
84	470.0
85	355.0
86	240.0
87	186.5
88	133.0
89	133.0
90	98.0
91	63.0
92	40.0
93	17.0
94	10.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	342273.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.81493279388869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.76457872264061	51.03052866462171
2	6.445281570805095	7.581576048491819
3	2.070630642728318	3.653520062790977
4	1.1373858686091696	2.6758109369186807
5	0.6858229374298375	2.016831498672161
6	0.46616250783104035	1.6450389941467944
7	0.3528492323523245	1.4526962721024026
8	0.26534846285832275	1.2485161607979136
9	0.20968077724291445	1.1099124739541122
>10	1.4299794441858698	15.791778669711793
>50	0.10886088018262033	4.385812065999697
>100	0.06142150579111116	6.778874191076047
>500	0.0019974473428003626	0.6291039607159522
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	562	0.16419641631095647	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	538	0.15718446970692984	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	530	0.1548471541722543	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	512	0.14958819421923436	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	474	0.13848594542952555	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	451	0.13176616326733337	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	447	0.13059750549999563	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	439	0.1282601899653201	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.12475421666330677	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	422	0.12329339445413456	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	417	0.12183257224496234	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	381	0.11131465233892243	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	368	0.10751651459507468	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	366	0.10693218571140581	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	355	0.10371837685122694	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	351	0.10254971908388918	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.921644418344421E-4	0.0	0.0	0.0	0.0
9	2.921644418344421E-4	0.0	0.0	0.0	0.0
10	2.921644418344421E-4	0.0	0.0	0.0	0.0
11	2.921644418344421E-4	0.0	0.0	0.0	0.0
12	2.921644418344421E-4	0.0	0.0	0.0	0.0
13	5.843288836688842E-4	0.0	0.0	0.0	0.0
14	5.843288836688842E-4	0.0	0.0	2.921644418344421E-4	0.0
15	5.843288836688842E-4	0.0	0.0	2.921644418344421E-4	0.0
16	5.843288836688842E-4	0.0	0.0	5.843288836688842E-4	0.0
17	5.843288836688842E-4	0.0	0.0	5.843288836688842E-4	0.0
18	8.764933255033263E-4	0.0	0.0	0.0014608222091722104	0.0
19	8.764933255033263E-4	0.0	0.0	0.002045151092841095	0.0
20	8.764933255033263E-4	0.0	0.0	0.002337315534675537	0.0
21	8.764933255033263E-4	0.0	0.0	0.004382466627516631	0.0
22	8.764933255033263E-4	0.0	0.0	0.006719782162192168	0.0
23	8.764933255033263E-4	0.0	0.0	0.00818060437136438	0.0
24	8.764933255033263E-4	0.0	0.0	0.00993359102237103	0.0
25	8.764933255033263E-4	0.0	0.0	0.011686577673377684	0.0
26	8.764933255033263E-4	0.0	0.0	0.015484715417225431	0.0
27	8.764933255033263E-4	0.0	0.0	0.04119518629865634	0.0
28	8.764933255033263E-4	0.0	0.0	0.1416997542897044	0.0
29	8.764933255033263E-4	0.0	0.0	0.28135435748656773	0.0
30	8.764933255033263E-4	0.0	0.0	0.46541795584226625	0.0
31	8.764933255033263E-4	0.0	0.0	1.0523763194876605	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCC	35	2.383446E-5	31.714285	19
CGCCGTT	35	2.383446E-5	31.714285	25
CACTATA	25	0.005491018	29.6	2
GGTATCA	345	0.0	28.95652	1
TAGAACA	45	3.997453E-6	28.777777	4
TTTACCG	40	5.928133E-5	27.75	30
GACCGTT	40	5.928133E-5	27.75	7
GGACCGT	50	9.066753E-6	25.900002	6
CCGTTTA	45	1.3205613E-4	24.666666	27
GCGGTAA	240	0.0	23.125002	23
GTTTAAC	40	0.0019284323	23.125	33
ATACGGC	40	0.0019284323	23.125	29
TATACTA	40	0.0019284323	23.125	5
AGGACCG	65	2.6737162E-6	22.76923	5
GTTACGG	50	2.6967327E-4	22.2	17
TAGGACC	75	3.7249083E-7	22.2	4
TTAGGAC	85	5.1539246E-8	21.764706	3
CGCGGTA	265	0.0	21.64151	22
CTTATAC	460	0.0	21.315218	37
ACTTAGA	165	0.0	21.303032	1
>>END_MODULE