FastQCFastQC Report
Fri 10 Feb 2017
ERR1631642.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631642.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268951
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA9710.36103230700015987No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA9290.34541607950890685No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8380.3115809199445252No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8010.2978237671546118No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7570.2814639097828229No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7180.26696312711237363No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG6850.25469323408353195No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA6470.24056426635335063No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6380.23721793189093923No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6300.23424341236879578No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6020.2238325940412938No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5920.22011444463861446No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT5540.20598547690843313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4880.18144569085074977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4800.17847117132860632No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4700.174753021925927No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4660.1732657621648553No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4460.16582946335949672No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT4430.1647140185386929No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA4410.16397038865815708No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4300.15988042431520985No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC4130.153559570330655No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4040.15021323586824367No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4030.14984142092797573No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4010.14909779104743986No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3940.14649508646556436No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC3930.14612327152529642No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT3870.14389238188368883No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA3850.143148752003153No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3800.14128967730181335No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC3780.1405460474212775No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA3650.1357124531977944No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG3610.13422519343672265No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC3510.13050704403404337No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC3500.13013522909377545No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT3490.1297634141535075No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC3450.12827615439243578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3450.12827615439243578No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3420.127160709571632No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3360.12492981993002443No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG3340.12418619004948855No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA3330.12381437510922064No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA3310.12307074522868479No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3290.12232711534814891No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3280.121955300407881No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC3170.11786533606493377No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3150.11712170618439789No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3150.11712170618439789No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG3130.11637807630386204No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT3120.11600626136359413No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3060.11377537172198654No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3020.11228811196091482No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2990.11117266714011102No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2980.11080085219984309No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC2920.1085699625582355No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA2870.10671088785689586No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2870.10671088785689586No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG2830.10522362809582415No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2770.10299273845421657No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC2700.10039003387234105No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC2700.10039003387234105No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTC200.001839430437.08
GAGATCA200.001839430437.013
GATTAGA507.2886905E-933.3000032
GGGCTAA303.5922157E-430.8333321
ATTAGAT551.8446372E-830.2727283
TTAGATA551.8446372E-830.2727284
TAGATAC551.8446372E-830.2727285
ACTATTT250.00548895329.617
CTATCAA250.00548895329.69
TGCGCTC250.00548895329.612
TGCACCG250.00548895329.612
GGTATCA5700.027.2631571
AGATACC754.5838533E-1027.1333336
CCTGGTA701.9115578E-723.78571312
ACCCTGG753.71876E-722.210
TAGTCCA855.1435563E-821.76470617
GTCCACG806.9193993E-720.812519
GATGCTA450.003818305420.55555714
AGCCACG555.1290274E-420.18181822
AAGCCAC555.1290274E-420.18181821
Produced by FastQC (version 0.11.5)