##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631642.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	268951
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.60122104026384	34.0	31.0	34.0	31.0	34.0
2	32.76637008228265	34.0	31.0	34.0	31.0	34.0
3	32.84677878126499	34.0	31.0	34.0	31.0	34.0
4	36.26815293492123	37.0	37.0	37.0	35.0	37.0
5	36.19037296756658	37.0	35.0	37.0	35.0	37.0
6	36.2601998133489	37.0	37.0	37.0	35.0	37.0
7	36.25959375499626	37.0	37.0	37.0	35.0	37.0
8	36.24245308624991	37.0	37.0	37.0	35.0	37.0
9	38.086922896735835	39.0	38.0	39.0	37.0	39.0
10	38.010871868853435	39.0	38.0	39.0	35.0	39.0
11	38.089086859688194	39.0	38.0	39.0	37.0	39.0
12	38.00452870597246	39.0	38.0	39.0	35.0	39.0
13	38.06725016824626	39.0	38.0	39.0	35.0	39.0
14	39.42442303616644	40.0	39.0	41.0	37.0	41.0
15	39.445151719086375	40.0	39.0	41.0	37.0	41.0
16	39.377314083234495	40.0	39.0	41.0	36.0	41.0
17	39.3731646285011	40.0	39.0	41.0	37.0	41.0
18	39.410874099743076	40.0	39.0	41.0	37.0	41.0
19	39.41653312313395	41.0	39.0	41.0	37.0	41.0
20	39.41736227045075	40.0	39.0	41.0	37.0	41.0
21	39.37447713524025	40.0	39.0	41.0	37.0	41.0
22	39.34236719699871	40.0	39.0	41.0	36.0	41.0
23	39.28952485768783	40.0	39.0	41.0	36.0	41.0
24	39.309346312153515	40.0	39.0	41.0	36.0	41.0
25	39.25156998858528	40.0	39.0	41.0	36.0	41.0
26	39.18794129785723	40.0	39.0	41.0	36.0	41.0
27	39.05732642749051	40.0	39.0	41.0	35.0	41.0
28	39.07013545218274	40.0	39.0	41.0	35.0	41.0
29	39.050406951452125	40.0	39.0	41.0	35.0	41.0
30	39.01107636707058	40.0	39.0	41.0	35.0	41.0
31	38.923420994902415	40.0	38.0	41.0	35.0	41.0
32	38.87136690326491	40.0	38.0	41.0	35.0	41.0
33	38.8000230525263	40.0	38.0	41.0	35.0	41.0
34	38.78907682068481	40.0	38.0	41.0	35.0	41.0
35	38.699837516871106	40.0	38.0	41.0	35.0	41.0
36	38.65566218381787	40.0	38.0	41.0	35.0	41.0
37	38.6089399184238	40.0	38.0	41.0	35.0	41.0
38	38.57314157597481	40.0	38.0	41.0	35.0	41.0
39	38.48122520459117	40.0	38.0	41.0	34.0	41.0
40	38.15503567564352	40.0	37.0	41.0	34.0	41.0
41	38.2367494450662	40.0	37.0	41.0	34.0	41.0
42	38.230067930589584	40.0	37.0	41.0	34.0	41.0
43	37.76566735204554	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	0.0
19	3.0
20	5.0
21	11.0
22	33.0
23	63.0
24	106.0
25	193.0
26	341.0
27	545.0
28	868.0
29	1236.0
30	1784.0
31	2440.0
32	3332.0
33	4521.0
34	6981.0
35	10234.0
36	15736.0
37	28555.0
38	64283.0
39	127678.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.322207390937386	16.614178790932176	13.063346111373447	28.000267706756993
2	21.44702938453473	20.035991686217937	31.94001881383598	26.576960115411357
3	20.96181088748508	19.734449769660646	30.464657130852835	28.839082212001443
4	14.498179965867388	16.922041561474025	36.461288487494	32.11848998516459
5	15.943052823748562	33.019769400374045	34.40069008852914	16.636487687348254
6	30.81044502530201	35.763020029670834	16.395551606054635	17.030983338972526
7	28.611903283497735	28.222241226096944	20.491464988046147	22.674390502359167
8	26.5115206859242	29.839636216262445	21.266327323564514	22.38251577424884
9	28.665072819956052	12.155374027239162	18.08656595439318	41.092987198411606
10	19.89098385951344	22.61118196251362	28.934638651650303	28.563195526322637
11	39.02458068570111	19.55895311785418	18.648006514197753	22.768459682246952
12	21.997687311071534	25.107919286412766	26.411130652051863	26.483262750463837
13	36.28950998509023	17.68091585456087	20.117047343196344	25.912526817152564
14	23.298295972128752	21.18118170224316	23.881301798468865	31.639220527159225
15	30.342330015504682	23.96496016002915	20.213719227666004	25.47899059680016
16	23.941164003852002	24.568043993143732	23.376748924525284	28.11404307847898
17	26.639796840316638	24.151239445103386	21.474915505054824	27.734048209525152
18	26.617116128960294	20.704514948819675	23.43661112990842	29.241757792311613
19	28.256820015541866	22.363553212295177	23.624749489683992	25.754877282478965
20	30.98408260240713	20.16463965555064	22.83204003703277	26.019237705009463
21	27.378221311688744	21.97537841465546	21.977981119237334	28.668419154418462
22	28.43045759264699	23.174853411959802	21.34626753572212	27.04842145967109
23	27.926648348583942	21.984673788162155	22.5070737792386	27.581604084015304
24	27.491996683410736	21.404642481344187	23.423225792058776	27.680135043186304
25	27.65150529278568	22.04490780848556	23.48531888708352	26.818268011645248
26	27.725496465898992	23.012370283062715	22.48997029198627	26.77216295905202
27	26.318176916984875	21.906220835765623	25.173358715899923	26.60224353134958
28	25.816598562563442	23.118337541039075	22.259073214079887	28.805990682317596
29	27.080397544534137	23.940792188911736	22.404081040784384	26.57472922576975
30	26.557625738517427	21.75080219073363	24.531234314057208	27.16033775669174
31	27.983536034444935	22.69298124937256	23.087848715937103	26.2356340002454
32	24.981874021661937	22.355373283609282	23.422853977118507	29.23989871761027
33	25.066647828043028	21.921093433376342	25.49497863923168	27.51728009934895
34	25.716208528691098	22.08469200709423	24.74056612542805	27.458533338786616
35	25.082635870474547	24.40481723436611	25.02351729497195	25.489029600187397
36	25.919963115957927	22.87851690456626	25.43586006372908	25.765659915746735
37	26.382129086710965	22.945815408754754	24.571390327606142	26.10066517692814
38	25.33584184479701	21.90324631624348	25.568226182464464	27.192685656495048
39	24.93688441388952	20.795609609185316	26.721596127175584	27.545909849749584
40	24.759900502321987	21.817357065041588	27.592014902342804	25.830727530293622
41	22.803410286632136	20.71641302690825	28.684035381909716	27.7961413045499
42	20.812341281497375	21.533662265617156	30.03930083918632	27.614695613699148
43	20.230079085037794	22.243085171648367	29.16665117437749	28.360184568936347
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	5.5
2	11.0
3	12.5
4	14.0
5	14.0
6	22.5
7	31.0
8	33.0
9	35.0
10	52.5
11	70.0
12	70.0
13	159.0
14	248.0
15	510.0
16	772.0
17	713.5
18	655.0
19	655.0
20	759.5
21	864.0
22	614.5
23	365.0
24	356.5
25	348.0
26	348.0
27	379.0
28	410.0
29	513.5
30	617.0
31	844.5
32	1072.0
33	1072.0
34	1645.5
35	2219.0
36	2587.5
37	2956.0
38	4017.5
39	5079.0
40	5079.0
41	6504.5
42	7930.0
43	9884.0
44	11838.0
45	17465.5
46	23093.0
47	23093.0
48	26076.0
49	29059.0
50	32476.0
51	35893.0
52	35621.5
53	35350.0
54	35350.0
55	31194.0
56	27038.0
57	25236.5
58	23435.0
59	21729.0
60	20023.0
61	20023.0
62	17689.0
63	15355.0
64	11914.5
65	8474.0
66	7039.0
67	5604.0
68	5604.0
69	4726.0
70	3848.0
71	3150.0
72	2452.0
73	1854.5
74	1257.0
75	1257.0
76	1063.5
77	870.0
78	714.5
79	559.0
80	487.0
81	415.0
82	415.0
83	371.5
84	328.0
85	247.0
86	166.0
87	148.0
88	130.0
89	130.0
90	89.5
91	49.0
92	33.0
93	17.0
94	9.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	268951.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.7333248125447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63178006605044	30.04771822633403
2	8.316043193901816	6.2758391798129765
3	3.308729269332258	3.745480687094633
4	1.9226652051185673	2.9019420276206715
5	1.2377792206598162	2.3352762689687636
6	0.813854676692552	1.8425665719509168
7	0.6268880561840642	1.6558199451568643
8	0.44492055006014175	1.3430665304956306
9	0.3399392966687793	1.154433590797594
>10	2.8014997331325415	20.748491566352318
>50	0.33594001082531383	8.820476259092604
>100	0.20696304238345228	15.596925983892493
>500	0.01299767899026512	3.531963162430495
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	971	0.36103230700015987	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	929	0.34541607950890685	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	838	0.3115809199445252	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	801	0.2978237671546118	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	757	0.2814639097828229	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	718	0.26696312711237363	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	685	0.25469323408353195	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	647	0.24056426635335063	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	638	0.23721793189093923	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	630	0.23424341236879578	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	602	0.2238325940412938	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	592	0.22011444463861446	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	554	0.20598547690843313	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	488	0.18144569085074977	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	480	0.17847117132860632	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	470	0.174753021925927	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	466	0.1732657621648553	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	446	0.16582946335949672	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	443	0.1647140185386929	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	441	0.16397038865815708	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	430	0.15988042431520985	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	413	0.153559570330655	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	404	0.15021323586824367	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	403	0.14984142092797573	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	401	0.14909779104743986	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	394	0.14649508646556436	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	393	0.14612327152529642	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	387	0.14389238188368883	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	385	0.143148752003153	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	380	0.14128967730181335	No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC	378	0.1405460474212775	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	365	0.1357124531977944	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	361	0.13422519343672265	No Hit
CCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACCGGCTAAC	351	0.13050704403404337	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	350	0.13013522909377545	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	349	0.1297634141535075	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	345	0.12827615439243578	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	345	0.12827615439243578	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	342	0.127160709571632	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	336	0.12492981993002443	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	334	0.12418619004948855	No Hit
GAATTACTGGGCGTAAAGCGCACGCAGGCGGTTTGTTAAGTCA	333	0.12381437510922064	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	331	0.12307074522868479	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	329	0.12232711534814891	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	328	0.121955300407881	No Hit
AGTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCAC	317	0.11786533606493377	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	315	0.11712170618439789	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	315	0.11712170618439789	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	313	0.11637807630386204	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	312	0.11600626136359413	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	306	0.11377537172198654	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	302	0.11228811196091482	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	299	0.11117266714011102	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	298	0.11080085219984309	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	292	0.1085699625582355	No Hit
CATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCAGTA	287	0.10671088785689586	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	287	0.10671088785689586	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	283	0.10522362809582415	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	277	0.10299273845421657	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACC	270	0.10039003387234105	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	270	0.10039003387234105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	7.436298805358597E-4	0.0	0.0	0.0	0.0
9	7.436298805358597E-4	0.0	0.0	0.0	0.0
10	0.0011154448208037895	0.0	0.0	0.0	0.0
11	0.0011154448208037895	0.0	0.0	0.0	0.0
12	0.0011154448208037895	0.0	0.0	0.0	0.0
13	0.0011154448208037895	0.0	0.0	0.0	0.0
14	0.0011154448208037895	0.0	0.0	0.0	0.0
15	0.0014872597610717194	0.0	0.0	0.0	0.0
16	0.0014872597610717194	0.0	0.0	7.436298805358597E-4	0.0
17	0.0014872597610717194	0.0	0.0	0.0014872597610717194	0.0
18	0.002230889641607579	0.0	0.0	0.0018590747013396492	0.0
19	0.002230889641607579	0.0	0.0	0.0018590747013396492	0.0
20	0.002230889641607579	0.0	0.0	0.002230889641607579	0.0
21	0.002230889641607579	0.0	0.0	0.005949039044286878	0.0
22	0.002230889641607579	0.0	0.0	0.011154448208037895	0.0
23	0.002230889641607579	0.0	0.0	0.013385337849645474	0.0
24	0.002230889641607579	0.0	0.0	0.01673167231205684	0.0
25	0.002230889641607579	0.0	0.0	0.018218932073128562	0.0
26	0.002230889641607579	0.0	0.0	0.02156526653553993	0.0
27	0.002230889641607579	0.0	0.0	0.04461779283215158	0.0
28	0.002230889641607579	0.0	0.0	0.13422519343672268	0.0
29	0.002230889641607579	0.0	0.0	0.33203074165926133	0.0
30	0.002230889641607579	0.0	0.0	0.5714795631918081	0.0
31	0.002230889641607579	0.0	0.0	1.0994567783722686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTC	20	0.0018394304	37.0	8
GAGATCA	20	0.0018394304	37.0	13
GATTAGA	50	7.2886905E-9	33.300003	2
GGGCTAA	30	3.5922157E-4	30.833332	1
ATTAGAT	55	1.8446372E-8	30.272728	3
TTAGATA	55	1.8446372E-8	30.272728	4
TAGATAC	55	1.8446372E-8	30.272728	5
ACTATTT	25	0.005488953	29.6	17
CTATCAA	25	0.005488953	29.6	9
TGCGCTC	25	0.005488953	29.6	12
TGCACCG	25	0.005488953	29.6	12
GGTATCA	570	0.0	27.263157	1
AGATACC	75	4.5838533E-10	27.133333	6
CCTGGTA	70	1.9115578E-7	23.785713	12
ACCCTGG	75	3.71876E-7	22.2	10
TAGTCCA	85	5.1435563E-8	21.764706	17
GTCCACG	80	6.9193993E-7	20.8125	19
GATGCTA	45	0.0038183054	20.555557	14
AGCCACG	55	5.1290274E-4	20.181818	22
AAGCCAC	55	5.1290274E-4	20.181818	21
>>END_MODULE