FastQCFastQC Report
Fri 10 Feb 2017
ERR1631641.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631641.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347267
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7650.22029159119639932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7060.20330178220216719No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA6950.20013419069476798No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6940.19984622783045897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.1984064135089139No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6850.19725456205167782No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5950.17133790426386614No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5600.16125920401305047No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5580.16068327828443243No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5500.1583795753699603No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5250.15118050376223482No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC4910.1413897663757282No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4770.13735828627540192No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA4570.13159902898922154No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4380.12612773456735019No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4250.12238421733133295No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3720.10712218552295495No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACT308.295276E-637.04
GGTATCA5100.030.8333341
CTCTAAT358.8568823E-426.4285721
ATGCTAG451.320616E-424.66666615
TATACAC952.8012437E-1023.36842237
TGCGCTA400.001928488523.1250025
TCTAATA400.001928488523.1250022
AGTCGGT1054.0017767E-1122.9047611
CTTATAC5450.022.74311837
GGGCAGT1155.456968E-1222.521747
GCAGTCG1107.2759576E-1121.8636369
TTATACA2150.021.51162737
CTAATAC705.0850194E-621.1428593
GTATCAA7400.021.02
GTGATTC1251.8189894E-1120.7216
ATTAGCT450.003820514320.5555553
CTTTTTA450.003820514320.5555552
CTCTATG1800.020.5555551
TAAGACT450.003820514320.5555554
GCGGTAA2550.020.31372523
Produced by FastQC (version 0.11.5)