##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631641.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	347267
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.62212649056778	34.0	31.0	34.0	31.0	34.0
2	32.7883357762183	34.0	31.0	34.0	31.0	34.0
3	32.88046661502533	34.0	31.0	34.0	31.0	34.0
4	36.301258109754166	37.0	37.0	37.0	35.0	37.0
5	36.22387672885705	37.0	35.0	37.0	35.0	37.0
6	36.27539328528193	37.0	37.0	37.0	35.0	37.0
7	36.26535489983212	37.0	37.0	37.0	35.0	37.0
8	36.2434409258582	37.0	37.0	37.0	35.0	37.0
9	38.07387399320984	39.0	38.0	39.0	37.0	39.0
10	38.015135328148084	39.0	38.0	39.0	35.0	39.0
11	38.07871752858751	39.0	38.0	39.0	37.0	39.0
12	38.01832307705598	39.0	38.0	39.0	35.0	39.0
13	38.07037524440848	39.0	38.0	39.0	37.0	39.0
14	39.4670872844238	40.0	39.0	41.0	37.0	41.0
15	39.46528751652187	41.0	39.0	41.0	37.0	41.0
16	39.41028948906749	40.0	39.0	41.0	37.0	41.0
17	39.38677732119666	40.0	39.0	41.0	37.0	41.0
18	39.41475003383564	41.0	39.0	41.0	37.0	41.0
19	39.43888708112202	41.0	39.0	41.0	37.0	41.0
20	39.43431135120815	41.0	39.0	41.0	37.0	41.0
21	39.40125321438547	40.0	39.0	41.0	37.0	41.0
22	39.37861069436485	40.0	39.0	41.0	37.0	41.0
23	39.31413004978878	40.0	39.0	41.0	36.0	41.0
24	39.32269694500197	40.0	39.0	41.0	36.0	41.0
25	39.27060734247711	40.0	39.0	41.0	36.0	41.0
26	39.20769609551152	40.0	39.0	41.0	36.0	41.0
27	39.098552410681116	40.0	39.0	41.0	36.0	41.0
28	39.08691871096303	40.0	39.0	41.0	35.0	41.0
29	39.05413989811874	40.0	39.0	41.0	35.0	41.0
30	39.0153772169541	40.0	39.0	41.0	35.0	41.0
31	38.94044640003225	40.0	38.0	41.0	35.0	41.0
32	38.88481197464775	40.0	38.0	41.0	35.0	41.0
33	38.827228040671876	40.0	38.0	41.0	35.0	41.0
34	38.79991188336352	40.0	38.0	41.0	35.0	41.0
35	38.71721470799126	40.0	38.0	41.0	35.0	41.0
36	38.667785882332616	40.0	38.0	41.0	35.0	41.0
37	38.622797444041616	40.0	38.0	41.0	35.0	41.0
38	38.58255751338307	40.0	38.0	41.0	35.0	41.0
39	38.50056584702837	40.0	38.0	41.0	35.0	41.0
40	38.180607428865976	40.0	38.0	41.0	34.0	41.0
41	38.25538562546974	40.0	38.0	41.0	34.0	41.0
42	38.23901493663377	40.0	37.0	41.0	34.0	41.0
43	37.752835714306286	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	1.0
19	5.0
20	10.0
21	24.0
22	52.0
23	101.0
24	165.0
25	272.0
26	440.0
27	736.0
28	1068.0
29	1594.0
30	2411.0
31	3165.0
32	4273.0
33	5812.0
34	8666.0
35	12886.0
36	20019.0
37	36171.0
38	81716.0
39	167678.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.730809434815285	16.678233175049744	12.975030740035765	27.615926650099205
2	20.1219810693213	19.59961643346474	33.55285702355824	26.725545473655714
3	20.758091036579923	20.224207886151003	29.489413045293677	29.528288031975396
4	15.084070758235018	16.25982313320874	34.176584587651575	34.47952152090467
5	16.481842501590997	33.29657007432322	33.60670607918403	16.61488134490176
6	33.320758954925175	34.19242254518857	15.886911223928562	16.599907275957694
7	29.95648881120291	28.448427290816575	20.21614492595035	21.378938972030166
8	26.969450019725453	31.37355406646759	20.2305430691658	21.426452844641155
9	28.158736649321703	13.414750033835638	18.10566509342955	40.32084822341311
10	19.117278635747134	24.061025090204367	29.99507583502032	26.826620439028183
11	38.00274716572551	20.00535610927615	19.537416454773993	22.45448027022435
12	22.492779331177452	24.376632389487053	27.069085170776376	26.06150310855912
13	34.11841608906116	18.056711406497016	21.70088145432765	26.123991050114174
14	23.688689106652806	20.38575505302836	23.967725122168247	31.957830718150586
15	28.970503963808824	24.53357215053547	20.813955832255875	25.681968053399835
16	24.97242755574241	24.76394244198268	23.136376332908107	27.127253669366798
17	26.22564194121526	24.25914354084897	22.56908949021934	26.946125027716427
18	26.02090034469155	22.139448896670284	24.260583355170517	27.57906740346765
19	27.569564628945454	22.90168659849626	24.151733392461708	25.377015380096584
20	28.870004924164977	21.508522260969226	23.853691827901873	25.767780986963924
21	26.63685291144854	23.073024502760124	22.953807876936192	27.33631470885515
22	27.351288777799216	23.412820682644767	22.476941373640454	26.758949165915563
23	27.042016661531328	22.80377922463119	23.558241929120822	26.595962184716658
24	27.055262953289542	22.442385829923374	23.749737233886318	26.752613982900762
25	27.147699032732735	22.75799312920606	23.94900753598816	26.145300302073043
26	26.849369505308594	23.415700311287857	23.44046511761843	26.294465065785115
27	26.268548407997304	22.438066386958738	24.85234704132555	26.441038163718407
28	25.69723008520821	23.482795658671858	23.34630126098938	27.47367299513055
29	26.460331675627113	23.930577912672383	23.359835515611906	26.2492548960886
30	26.033570710721143	22.999018046632706	24.533860113399776	26.433551129246375
31	27.05871850766125	23.021767112913118	23.71201409866184	26.20750028076379
32	24.774021142233497	23.033861553214095	24.076287122012747	28.115830182539657
33	25.16853026633685	22.56908949021934	25.514661629236297	26.74771861420751
34	25.67966435048536	22.453040455902805	25.076958075486584	26.790337118125247
35	25.25693486567972	23.720652984591105	25.21057284452597	25.8118393052032
36	25.370968159946095	22.647415389311394	25.88498187273769	26.096634578004817
37	25.91521797349014	22.084447989587265	25.434319990094078	26.566014046828524
38	24.977034961571356	21.619675926592507	26.505253882459318	26.89803522937682
39	24.539907333550264	20.822306755320835	27.53184149372097	27.10594441740793
40	24.275845386978894	21.77344809613352	28.07522741867209	25.875479098215493
41	22.721133882574502	21.09644740214302	29.105846510034066	27.07657220524841
42	21.66546202201764	21.67150924216813	29.39467326293601	27.26835547287822
43	20.745708633414637	22.386233071383113	29.428940843788787	27.439117451413463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	5.0
2	5.0
3	17.0
4	29.0
5	29.0
6	38.0
7	47.0
8	55.5
9	64.0
10	110.0
11	156.0
12	156.0
13	318.0
14	480.0
15	875.0
16	1270.0
17	1244.5
18	1219.0
19	1219.0
20	1386.5
21	1554.0
22	1310.5
23	1067.0
24	1157.5
25	1248.0
26	1248.0
27	1394.5
28	1541.0
29	2040.5
30	2540.0
31	3059.5
32	3579.0
33	3579.0
34	4507.0
35	5435.0
36	6049.0
37	6663.0
38	8316.0
39	9969.0
40	9969.0
41	11777.5
42	13586.0
43	15331.5
44	17077.0
45	22056.5
46	27036.0
47	27036.0
48	29724.0
49	32412.0
50	36090.5
51	39769.0
52	40034.0
53	40299.0
54	40299.0
55	35834.0
56	31369.0
57	29081.0
58	26793.0
59	24781.0
60	22769.0
61	22769.0
62	20783.5
63	18798.0
64	15589.5
65	12381.0
66	10816.5
67	9252.0
68	9252.0
69	7870.5
70	6489.0
71	5623.5
72	4758.0
73	3839.5
74	2921.0
75	2921.0
76	2415.0
77	1909.0
78	1550.0
79	1191.0
80	940.5
81	690.0
82	690.0
83	548.0
84	406.0
85	335.5
86	265.0
87	201.0
88	137.0
89	137.0
90	105.0
91	73.0
92	44.5
93	16.0
94	8.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	347267.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.367538670645345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.85604205539371	47.221492252258166
2	6.191848622660775	6.732711388705835
3	2.060923392054085	3.361419966442141
4	1.0814186755688644	2.3517628665259322
5	0.686813153098977	1.8670170330308247
6	0.4341395974970383	1.4161860813227183
7	0.3500625057661744	1.3322425773569846
8	0.27296252835214685	1.1872240652657977
9	0.217846689747729	1.0659409496218561
>10	1.5923023233580211	16.869722294215244
>50	0.16669888101099326	6.242472410468715
>100	0.08308314836330653	8.318567182167804
>500	0.005858427128181871	2.0332409326179928
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	765	0.22029159119639932	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	706	0.20330178220216719	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	695	0.20013419069476798	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	694	0.19984622783045897	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.1984064135089139	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	685	0.19725456205167782	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	595	0.17133790426386614	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	560	0.16125920401305047	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	558	0.16068327828443243	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	550	0.1583795753699603	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	525	0.15118050376223482	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	491	0.1413897663757282	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	477	0.13735828627540192	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	457	0.13159902898922154	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	438	0.12612773456735019	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	425	0.12238421733133295	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	372	0.10712218552295495	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.8796286430901873E-4	0.0
11	2.8796286430901873E-4	0.0	0.0	2.8796286430901873E-4	0.0
12	2.8796286430901873E-4	0.0	0.0	2.8796286430901873E-4	0.0
13	2.8796286430901873E-4	0.0	0.0	5.759257286180375E-4	0.0
14	2.8796286430901873E-4	0.0	0.0	8.638885929270561E-4	0.0
15	2.8796286430901873E-4	0.0	0.0	0.0014398143215450935	0.0
16	2.8796286430901873E-4	0.0	0.0	0.0017277771858541123	0.0
17	2.8796286430901873E-4	0.0	0.0	0.0034555543717082245	0.0
18	2.8796286430901873E-4	0.0	0.0	0.00431944296463528	0.0
19	2.8796286430901873E-4	0.0	0.0	0.004895368693253318	0.0
20	2.8796286430901873E-4	0.0	0.0	0.005183331557562337	0.0
21	2.8796286430901873E-4	0.0	0.0	0.008350923064961543	0.0
22	2.8796286430901873E-4	0.0	0.0	0.011806477436669767	0.0
23	2.8796286430901873E-4	0.0	0.0	0.013822217486832898	0.0
24	2.8796286430901873E-4	0.0	0.0	0.01814166045146818	0.0
25	2.8796286430901873E-4	0.0	0.0	0.018717586180086215	0.0
26	2.8796286430901873E-4	0.0	0.0	0.021597214823176402	0.0
27	2.8796286430901873E-4	0.0	0.0	0.043194429646352804	0.0
28	5.759257286180375E-4	0.0	0.0	0.1399499520541831	0.0
29	5.759257286180375E-4	0.0	0.0	0.307832301946341	0.0
30	5.759257286180375E-4	0.0	0.0	0.538490556257865	0.0
31	5.759257286180375E-4	0.0	0.0	1.230753282056746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACT	30	8.295276E-6	37.0	4
GGTATCA	510	0.0	30.833334	1
CTCTAAT	35	8.8568823E-4	26.428572	1
ATGCTAG	45	1.320616E-4	24.666666	15
TATACAC	95	2.8012437E-10	23.368422	37
TGCGCTA	40	0.0019284885	23.125002	5
TCTAATA	40	0.0019284885	23.125002	2
AGTCGGT	105	4.0017767E-11	22.90476	11
CTTATAC	545	0.0	22.743118	37
GGGCAGT	115	5.456968E-12	22.52174	7
GCAGTCG	110	7.2759576E-11	21.863636	9
TTATACA	215	0.0	21.511627	37
CTAATAC	70	5.0850194E-6	21.142859	3
GTATCAA	740	0.0	21.0	2
GTGATTC	125	1.8189894E-11	20.72	16
ATTAGCT	45	0.0038205143	20.555555	3
CTTTTTA	45	0.0038205143	20.555555	2
CTCTATG	180	0.0	20.555555	1
TAAGACT	45	0.0038205143	20.555555	4
GCGGTAA	255	0.0	20.313725	23
>>END_MODULE