FastQCFastQC Report
Fri 10 Feb 2017
ERR1631640.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631640.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995868
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.1314431229841706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12590.1264223772628501No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA11940.11989540782513346No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11560.11607964107692988No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA11400.11447300244610732No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA11360.11407134278840168No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10050.10091698899854198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.030.3789461
TATCTAT601.3371064E-624.6666687
ATACGGC903.8271537E-922.6111129
TCTATAG502.701751E-422.21
GATATAT603.7256417E-521.5833341
TTCTATA450.003825455220.5555552
GTACTAT909.475116E-820.5555551
CTTATAC6950.019.9640337
TCTATCG759.263733E-619.73333431
TCTTATA15000.019.48666637
AGCGTGT701.2189899E-418.56
TAGACTG609.2356314E-418.55
TATTGCG801.6163169E-518.511
GTATCAA15600.018.0256422
TACGGCT1258.574716E-917.7630
CTGTACT852.7225235E-517.4117644
GGTAACG4250.016.54117625
CTATCGC904.4449982E-516.44444532
ATACACA1708.54925E-1116.3235337
CTCTATG2500.016.2799991
Produced by FastQC (version 0.11.5)