FastQCFastQC Report
Fri 10 Feb 2017
ERR1631639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590491
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15290.2589370540787243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15040.2547032892965346No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11680.19780149062390454No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9640.16325397000123626No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA8850.1498752732895167No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA8640.1463189108724773No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA8500.14394800259445106No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA8060.13649657657779712No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA7650.12955320233500595No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC7290.12345658104865273No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG7160.12125502336191407No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC6970.11803736212744986No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT6910.11702125857972433No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT6520.11041658551950834No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA5970.10110230299869093No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5910.10008619945096539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8000.030.0624981
AACGACC652.6791786E-622.7692337
CCGCTTA753.7343852E-722.225
ATACGGC555.1390234E-420.18181829
GCGGTAA3600.020.04166823
GCTTATT851.2432101E-619.58823427
GGTAATA3900.018.525
TATTAGA609.229564E-418.52
CGGTAAT3900.018.524
ATACACA1409.276846E-1118.537
GTATCAA13000.018.3576932
AGCCGCG4050.018.27160519
TAATACG3550.018.23943727
CTTATAC6100.018.19672237
TCTATGG3600.017.9861132
CTCTATG3650.017.7397251
CGTGCCA4600.017.69565210
CAGTCGG2300.017.69565210
GTAACGT2550.017.41176426
CGCGGTA4150.017.38554222
Produced by FastQC (version 0.11.5)