FastQCFastQC Report
Fri 10 Feb 2017
ERR1631638.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631638.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921224
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43210.4690498727779563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41970.4555895200298733No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34050.3696169444130852No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA21930.23805285142375793No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA20800.22578656222590815No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG17550.19050741187811No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA17470.18963900202339498No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT16730.18160621086728093No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC15950.17313921478380936No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC15700.1704254339878249No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15390.16706034580080414No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT13960.15153751964777298No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA13610.14773822653339472No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA12770.13861992305888687No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA11340.12309709690585567No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA10490.11387024219950848No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.11354458850399035No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT10020.10876833430305767No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA9830.10670586089810948No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA9700.10529469488419754No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9490.10301511901557059No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC9250.1004098894514255No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16950.029.7964611
CTTATAC10000.021.46000137
CCGTTAA450.003825239820.55555516
GCAGTCG4950.019.8080799
CAGTCGG5100.019.58823610
AGTCGGT5050.019.41584211
GCGGTAA4450.019.12359623
TATACAC2850.018.8245637
GATCGCA500.007033847718.520
GAGAACG609.234917E-418.57
GTCGGTG5200.018.512
TCTATAC609.234917E-418.53
GTATCAA27850.018.0682222
AACTCCG5050.017.584165
CTCTATG6900.017.4275361
ATACACA1705.456968E-1217.41176437
CGTTATT752.0667055E-417.26666610
CGTGCCA5050.017.21782310
TCTATGG7200.017.2152772
GGGCAGT5700.017.2017547
Produced by FastQC (version 0.11.5)