##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631638.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	921224
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.68309010620653	34.0	31.0	34.0	31.0	34.0
2	32.848833725565115	34.0	31.0	34.0	31.0	34.0
3	32.928824042795235	34.0	31.0	34.0	31.0	34.0
4	36.32962232855419	37.0	37.0	37.0	35.0	37.0
5	36.257625724036714	37.0	37.0	37.0	35.0	37.0
6	36.31667867966966	37.0	37.0	37.0	35.0	37.0
7	36.30626861653626	37.0	37.0	37.0	35.0	37.0
8	36.28925321094544	37.0	37.0	37.0	35.0	37.0
9	38.114783158059275	39.0	39.0	39.0	37.0	39.0
10	38.066618976492144	39.0	38.0	39.0	37.0	39.0
11	38.13554683768552	39.0	38.0	39.0	37.0	39.0
12	38.07963209816505	39.0	38.0	39.0	37.0	39.0
13	38.12012062212882	39.0	38.0	39.0	37.0	39.0
14	39.52405278195097	41.0	39.0	41.0	37.0	41.0
15	39.53302779779945	41.0	39.0	41.0	37.0	41.0
16	39.48108711887662	41.0	39.0	41.0	37.0	41.0
17	39.466845197259296	41.0	39.0	41.0	37.0	41.0
18	39.49614860229434	41.0	39.0	41.0	37.0	41.0
19	39.525775490000264	41.0	39.0	41.0	37.0	41.0
20	39.52689790973748	41.0	39.0	41.0	37.0	41.0
21	39.48756111434353	41.0	39.0	41.0	37.0	41.0
22	39.467668015596644	41.0	39.0	41.0	37.0	41.0
23	39.39992444834264	41.0	39.0	41.0	36.0	41.0
24	39.41465919255252	41.0	39.0	41.0	36.0	41.0
25	39.36779002718123	41.0	39.0	41.0	36.0	41.0
26	39.29971103662084	41.0	39.0	41.0	36.0	41.0
27	39.20154055908226	40.0	39.0	41.0	36.0	41.0
28	39.1826754405009	40.0	39.0	41.0	35.0	41.0
29	39.161423280331384	40.0	39.0	41.0	35.0	41.0
30	39.091774638958604	40.0	39.0	41.0	35.0	41.0
31	38.989992661936725	40.0	39.0	41.0	35.0	41.0
32	38.92248247983118	40.0	38.0	41.0	35.0	41.0
33	38.84818893124799	40.0	38.0	41.0	35.0	41.0
34	38.810142810000606	40.0	38.0	41.0	35.0	41.0
35	38.73275772233463	40.0	38.0	41.0	35.0	41.0
36	38.674422290344154	40.0	38.0	41.0	35.0	41.0
37	38.62222217397723	40.0	38.0	41.0	35.0	41.0
38	38.57246446032669	40.0	38.0	41.0	35.0	41.0
39	38.49021084991273	40.0	38.0	41.0	35.0	41.0
40	38.159857971568265	40.0	38.0	41.0	34.0	41.0
41	38.21218075082716	40.0	38.0	41.0	34.0	41.0
42	38.177521428013165	40.0	38.0	41.0	34.0	41.0
43	37.72036442819553	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	0.0
15	2.0
16	2.0
17	2.0
18	6.0
19	16.0
20	25.0
21	75.0
22	151.0
23	249.0
24	458.0
25	767.0
26	1203.0
27	1863.0
28	3085.0
29	4592.0
30	6334.0
31	8576.0
32	11158.0
33	14658.0
34	21619.0
35	32331.0
36	49530.0
37	94978.0
38	200823.0
39	468719.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.826309344958446	17.57878648406902	14.160073988519622	26.434830182452913
2	18.792932012192473	20.695835106336787	35.192960669717685	25.318272211753058
3	20.179022691549502	21.618520576971505	30.98084722065426	27.221609510824727
4	14.869781942285481	17.378618012557208	34.520811442168245	33.230788602989065
5	15.560819084174968	34.28916311342301	34.264630535027315	15.885387267374709
6	31.645289310743095	34.69850980868931	16.85572672878692	16.800474151780676
7	28.17913992687989	29.805671584761146	21.618629128203345	20.39655936015562
8	25.91259020607366	32.73481802471495	21.114083002613913	20.238508766597484
9	27.55649006104921	14.453162314485946	19.659387944734398	38.33095967973045
10	18.49463322709786	25.672149227549433	30.901062065252315	24.932155480100388
11	35.85675145241548	21.409233801985184	20.551027763063054	22.182986982536278
12	21.407931187203115	25.90542582477226	28.02206629440831	24.66457669361632
13	32.79007060172119	19.192074891665868	23.31191979366582	24.705934712947123
14	22.82897536321242	22.24692365808967	25.39729750853213	29.52680347016578
15	27.775220793205563	26.208826517763324	22.499956579507263	23.51599610952385
16	23.465736889182217	26.22879994442177	24.581860654954713	25.7236025114413
17	24.72873047163339	25.857988936458455	24.08816965254922	25.325110939358943
18	24.468207515218882	23.35729420857468	26.053815358696692	26.120682917509747
19	25.87459727492988	24.385600027789113	26.56606862174672	23.173734075534288
20	27.251352548348716	22.625550354745425	26.282098599254905	23.84099849765095
21	24.871692443965856	24.806561704862226	24.958859083132875	25.362886768039044
22	25.63828124321555	24.648076906376733	24.693885526212952	25.01975632419477
23	24.953322970309067	24.357702361206396	25.757253393311508	24.93172127517303
24	25.703411982319174	23.889520898283152	26.12426510816045	24.282802011237223
25	25.171402395074377	23.789219560063568	26.600479362239803	24.438898682622252
26	25.123531301833214	24.7731279254557	25.633396437782775	24.46994433492831
27	24.558739242572926	23.852287825762247	26.95891552977343	24.6300574018914
28	23.738743237258255	24.86865300947435	25.631333964377827	25.761269788889564
29	24.69605655084974	25.538305558691484	25.54047658332827	24.225161307130513
30	24.11726138268217	24.706151815410802	26.350160221618196	24.82642658028883
31	25.191810026660182	24.52335154099329	26.035361649283995	24.249476783062534
32	23.266871032452478	24.542565109028857	25.922034163243683	26.268529695274985
33	23.50351271786232	23.81831129019652	27.650712530285794	25.027463461655366
34	23.914053476678852	23.95627990586437	26.6746198535861	25.455046763870676
35	23.574505223485275	25.15403419798008	27.10057488732382	24.170885691210824
36	23.76826917231857	24.2733580540672	27.431221939506568	24.527150834107665
37	24.371379816418155	23.770223094491676	26.858397089090168	25.0
38	23.511979713945795	23.438273427526855	27.829170755429733	25.22057610309762
39	23.303669900046025	22.398352626505606	28.80005297300114	25.497924500447233
40	22.914079528974497	23.40038904761491	29.16554497060433	24.51998645280627
41	21.43430913654008	23.22258212986201	29.884045574149177	25.459063159448736
42	20.732308320234818	23.115550615268383	30.046655319444564	26.10548574505224
43	19.784981719972556	24.278677064427328	29.83313504641651	26.103206169183608
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	53.5
2	59.0
3	134.0
4	209.0
5	209.0
6	307.0
7	405.0
8	440.0
9	475.0
10	739.5
11	1004.0
12	1004.0
13	2002.0
14	3000.0
15	5694.0
16	8388.0
17	8296.5
18	8205.0
19	8205.0
20	9035.5
21	9866.0
22	8415.5
23	6965.0
24	7151.0
25	7337.0
26	7337.0
27	7635.5
28	7934.0
29	11119.5
30	14305.0
31	14970.0
32	15635.0
33	15635.0
34	18060.5
35	20486.0
36	21552.5
37	22619.0
38	27934.5
39	33250.0
40	33250.0
41	39044.5
42	44839.0
43	46961.0
44	49083.0
45	64153.5
46	79224.0
47	79224.0
48	85281.0
49	91338.0
50	101184.0
51	111030.0
52	108772.0
53	106514.0
54	106514.0
55	89337.5
56	72161.0
57	64551.5
58	56942.0
59	50191.5
60	43441.0
61	43441.0
62	39421.5
63	35402.0
64	28926.5
65	22451.0
66	19208.5
67	15966.0
68	15966.0
69	13681.5
70	11397.0
71	9698.0
72	7999.0
73	6445.0
74	4891.0
75	4891.0
76	4025.0
77	3159.0
78	2600.5
79	2042.0
80	1645.0
81	1248.0
82	1248.0
83	1035.0
84	822.0
85	675.5
86	529.0
87	432.0
88	335.0
89	335.0
90	255.5
91	176.0
92	107.0
93	38.0
94	21.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	921224.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.118248131848
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.01721981058668	34.95759139651566
2	6.843964506604176	5.628236615762224
3	2.3748067615554023	2.929436810604762
4	1.2284907270160912	2.0205354616448794
5	0.7614343424105768	1.5654423113674303
6	0.5212813760060077	1.2860506179075704
7	0.4025648150944093	1.15869319693424
8	0.29567743509868744	0.9726190514700969
9	0.2276661420888133	0.8425109629465547
>10	1.8206908857112694	15.22355200514966
>50	0.26624121619029545	7.763246374407943
>100	0.21872640804144278	17.379021675956437
>500	0.01645756953706696	4.524983078504555
>1k	0.004778004059148472	3.7480804408279806
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4321	0.4690498727779563	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4197	0.4555895200298733	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3405	0.3696169444130852	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	2193	0.23805285142375793	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	2080	0.22578656222590815	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	1755	0.19050741187811	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	1747	0.18963900202339498	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	1673	0.18160621086728093	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	1595	0.17313921478380936	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	1570	0.1704254339878249	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1539	0.16706034580080414	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	1396	0.15153751964777298	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	1361	0.14773822653339472	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	1277	0.13861992305888687	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	1134	0.12309709690585567	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	1049	0.11387024219950848	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1046	0.11354458850399035	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	1002	0.10876833430305767	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	983	0.10670586089810948	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	970	0.10529469488419754	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	949	0.10301511901557059	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	925	0.1004098894514255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.0855123183937891E-4	0.0	0.0	0.0	0.0
9	1.0855123183937891E-4	0.0	0.0	0.0	0.0
10	2.1710246367875782E-4	0.0	0.0	0.0	0.0
11	2.1710246367875782E-4	0.0	0.0	1.0855123183937891E-4	0.0
12	2.1710246367875782E-4	0.0	0.0	1.0855123183937891E-4	0.0
13	3.2565369551813676E-4	0.0	0.0	2.1710246367875782E-4	0.0
14	3.2565369551813676E-4	0.0	0.0	4.3420492735751564E-4	0.0
15	3.2565369551813676E-4	0.0	0.0	6.513073910362735E-4	0.0
16	3.2565369551813676E-4	0.0	0.0	0.001194063550233168	0.0
17	3.2565369551813676E-4	0.0	0.0	0.002279575868626957	0.0
18	5.427561591968945E-4	0.0	0.0	0.0030394344915026096	0.0
19	5.427561591968945E-4	0.0	0.0	0.004124946809896399	0.0
20	5.427561591968945E-4	0.0	0.0	0.005427561591968945	0.0
21	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.00900975224266845	0.0
22	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.014220211370958637	0.0
23	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.01671688970326435	0.0
24	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.019647772962927582	0.0
25	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.02171024636787578	0.0
26	5.427561591968945E-4	0.0	1.0855123183937891E-4	0.02594374440961156	0.0
27	6.513073910362735E-4	0.0	1.0855123183937891E-4	0.045157312445181626	0.0
28	6.513073910362735E-4	0.0	1.0855123183937891E-4	0.13937978168176252	0.0
29	6.513073910362735E-4	0.0	1.0855123183937891E-4	0.2989500924856495	0.0
30	6.513073910362735E-4	0.0	1.0855123183937891E-4	0.50161524232977	0.0
31	6.513073910362735E-4	0.0	1.0855123183937891E-4	1.134143270257831	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1695	0.0	29.796461	1
CTTATAC	1000	0.0	21.460001	37
CCGTTAA	45	0.0038252398	20.555555	16
GCAGTCG	495	0.0	19.808079	9
CAGTCGG	510	0.0	19.588236	10
AGTCGGT	505	0.0	19.415842	11
GCGGTAA	445	0.0	19.123596	23
TATACAC	285	0.0	18.82456	37
GATCGCA	50	0.0070338477	18.5	20
GAGAACG	60	9.234917E-4	18.5	7
GTCGGTG	520	0.0	18.5	12
TCTATAC	60	9.234917E-4	18.5	3
GTATCAA	2785	0.0	18.068222	2
AACTCCG	505	0.0	17.58416	5
CTCTATG	690	0.0	17.427536	1
ATACACA	170	5.456968E-12	17.411764	37
CGTTATT	75	2.0667055E-4	17.266666	10
CGTGCCA	505	0.0	17.217823	10
TCTATGG	720	0.0	17.215277	2
GGGCAGT	570	0.0	17.201754	7
>>END_MODULE