FastQCFastQC Report
Fri 10 Feb 2017
ERR1631637.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631637.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241963
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA5170.2136690320420891No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA4850.20044386951724025No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4710.19465786091261889No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA4480.18515227534788375No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA4350.17977955307216392No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4250.17564668978314865No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG3960.1636613862450044No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT3760.15539565966697388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3720.15374251435136777No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC3680.15208936903576167No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC3580.1479565057467464No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT3570.14754321941784487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.14217049714212504No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA3360.13886420651091283No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3100.12811876195947314No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3080.1272921893016701No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2880.11902646272363955No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2810.11613345842132888No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2770.11448031310572278No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2750.11365374044791973No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2730.11282716779011667No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2690.11117402247451057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2680.11076073614560904No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA2630.10869430450110142No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2560.10580130019879072No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2530.10456144121208613No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTTAT200.001839065937.01
ATACGGC352.3805791E-531.71428529
GGTATCA3150.030.5396821
TTATCCT250.005487877429.5999984
TCAAGAT250.005487877429.59999813
AACTCCG1600.026.593755
CGTGCCA1850.025.010
CTTTGTT451.3189987E-424.6666669
GCGGTAA1750.024.31428723
CTAACTC1750.024.3142873
TCCGTGC1750.024.3142878
CCGCGGT1750.024.31428721
CGGTAAT1750.024.31428724
TAACTCC1750.024.3142874
GGTAATA1950.023.71794925
CGCGGTA1800.023.6388922
CCGTGCC1900.023.3684229
ACAATAT400.001926807623.12535
TTAAACC400.001926807623.1254
TGTAGGC400.001926807623.12510
Produced by FastQC (version 0.11.5)