##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631637.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	241963
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5894124308262	34.0	31.0	34.0	31.0	34.0
2	32.75474762670326	34.0	31.0	34.0	31.0	34.0
3	32.83871914300947	34.0	31.0	34.0	31.0	34.0
4	36.27023553187884	37.0	37.0	37.0	35.0	37.0
5	36.19128131160549	37.0	35.0	37.0	35.0	37.0
6	36.23534589999297	37.0	37.0	37.0	35.0	37.0
7	36.23112624657489	37.0	37.0	37.0	35.0	37.0
8	36.21558668060819	37.0	37.0	37.0	35.0	37.0
9	38.04045659873617	39.0	38.0	39.0	35.0	39.0
10	37.977306447680014	39.0	38.0	39.0	35.0	39.0
11	38.04561854498415	39.0	38.0	39.0	35.0	39.0
12	37.992308741419144	39.0	38.0	39.0	35.0	39.0
13	38.0372866925935	39.0	38.0	39.0	35.0	39.0
14	39.430474907320544	40.0	39.0	41.0	37.0	41.0
15	39.43102457813798	40.0	39.0	41.0	37.0	41.0
16	39.37979360480735	40.0	39.0	41.0	36.0	41.0
17	39.35252910568971	40.0	39.0	41.0	36.0	41.0
18	39.380397002847545	40.0	39.0	41.0	36.0	41.0
19	39.38809652715498	40.0	39.0	41.0	37.0	41.0
20	39.39097713286742	40.0	39.0	41.0	37.0	41.0
21	39.34972289151647	40.0	39.0	41.0	36.0	41.0
22	39.32435950951179	40.0	39.0	41.0	36.0	41.0
23	39.259134661084545	40.0	39.0	41.0	36.0	41.0
24	39.2728846972471	40.0	39.0	41.0	36.0	41.0
25	39.21611568710919	40.0	39.0	41.0	36.0	41.0
26	39.15647846984869	40.0	39.0	41.0	36.0	41.0
27	39.041307968573705	40.0	39.0	41.0	35.0	41.0
28	39.04435388881771	40.0	39.0	41.0	35.0	41.0
29	39.020383281741424	40.0	39.0	41.0	35.0	41.0
30	38.978612432479345	40.0	38.0	41.0	35.0	41.0
31	38.8976248434678	40.0	38.0	41.0	35.0	41.0
32	38.844736591958274	40.0	38.0	41.0	35.0	41.0
33	38.78115249025677	40.0	38.0	41.0	35.0	41.0
34	38.754040907080835	40.0	38.0	41.0	35.0	41.0
35	38.68553456520212	40.0	38.0	41.0	35.0	41.0
36	38.651004492422395	40.0	38.0	41.0	35.0	41.0
37	38.60405103259589	40.0	38.0	41.0	35.0	41.0
38	38.55667188785062	40.0	38.0	41.0	35.0	41.0
39	38.49277368853916	40.0	38.0	41.0	34.0	41.0
40	38.15306472477197	40.0	38.0	41.0	34.0	41.0
41	38.23346544719647	40.0	37.0	41.0	34.0	41.0
42	38.220198956038736	40.0	37.0	41.0	34.0	41.0
43	37.76425321226799	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	7.0
21	16.0
22	41.0
23	62.0
24	125.0
25	181.0
26	326.0
27	522.0
28	799.0
29	1149.0
30	1656.0
31	2328.0
32	3033.0
33	4242.0
34	6162.0
35	9375.0
36	14360.0
37	25582.0
38	57545.0
39	114447.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.62717853556122	16.56120977174196	13.056128416328116	27.755483276368704
2	20.791195348049083	19.6327537681381	32.630195525762204	26.945855358050615
3	21.203241817963903	19.811293462223563	29.351595078586396	29.63386964122614
4	15.632555390700231	16.418625988270936	33.81178114009167	34.13703748093717
5	17.047234494530155	32.893045630943575	33.176973338898925	16.882746535627348
6	33.55347718452821	33.3596458962734	16.077251480598274	17.009625438600118
7	30.176927877402743	27.987336906882458	20.083235866640766	21.752499349074032
8	27.336824225191453	30.627823262234312	20.561408149179833	21.473944363394402
9	28.36508061149845	13.152010844633272	17.88992531916037	40.592983224707915
10	19.422391026727226	24.000776978298337	29.895479887420805	26.681352107553636
11	38.34635874079921	20.012150618069704	19.01489070643032	22.62659993470076
12	22.551381822840682	24.04293218384629	27.019007038266178	26.386678955046843
13	34.86855428309287	17.875460297648814	21.237957869591632	26.018027549666684
14	24.071448940540495	20.514293507685057	23.607741679512984	31.80651587226146
15	29.353661510230904	24.355376648495845	20.34691254447994	25.94404929679331
16	25.114170348359043	24.40621086695073	22.957642284150882	27.521976500539342
17	26.34121745886768	24.02681401701913	22.321594623971432	27.310373900141755
18	26.397424399598286	21.89053698292714	23.529217277021694	28.18282134045288
19	27.738951823212638	22.84068225307175	23.764790484495563	25.655575439220048
20	29.460289383087495	21.21564040783095	23.264300740195814	26.05976946888574
21	27.26160611333138	22.598083178006554	22.371602269768516	27.76870843889355
22	27.934849543111962	23.172137888850774	21.780189533110434	27.112823034926826
23	27.55834569748267	22.414170761645373	22.855147274583302	27.17233626628865
24	27.33062493025793	22.054198369172145	23.246116141724148	27.369060558845774
25	27.62447151010692	22.34763166269223	23.45482573781942	26.573071089381433
26	27.38435215301513	23.28992449258771	22.75347883767353	26.572244516723632
27	26.581750102288364	22.232324776928706	24.62153304430843	26.5643920764745
28	26.234176299682183	23.38167405760385	22.696445324285115	27.687704318428853
29	26.854519079363374	23.766030343482267	22.66999499923542	26.709455577918938
30	26.451151622355486	22.82993680852031	23.808185549030224	26.910726020093982
31	27.747630836119576	22.62164049875394	23.01384922488149	26.616879440244993
32	25.41380293681265	22.696445324285115	23.461025032752943	28.428726706149288
33	25.58077061368887	22.116191318507376	25.14723325467117	27.15580481313259
34	25.782041055863914	22.26662754222753	24.258667647532885	27.69266375437567
35	25.175336725036473	23.72552828324992	25.12532907923939	25.973805912474223
36	25.910159817823384	22.543529382591554	25.11747663899026	26.4288341605948
37	26.327992296342828	22.177357695184803	24.907940470237186	26.586709538235187
38	25.292710042444504	21.78390911007055	25.71302223893736	27.210358608547587
39	24.76287696879275	20.75523943743465	26.959493806904362	27.52238978686824
40	24.563259671933313	21.89301670090055	27.50379190206767	26.039931725098462
41	23.27876576170737	20.892450498629955	28.408475676033113	27.420308063629562
42	21.750846203758424	21.3640101999066	29.276790253055218	27.608353343279756
43	21.17720477924311	22.112471741547264	28.977570950930513	27.732752528279114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	7.0
3	9.0
4	11.0
5	11.0
6	16.0
7	21.0
8	29.5
9	38.0
10	48.5
11	59.0
12	59.0
13	133.5
14	208.0
15	417.5
16	627.0
17	620.0
18	613.0
19	613.0
20	732.5
21	852.0
22	710.5
23	569.0
24	607.0
25	645.0
26	645.0
27	744.5
28	844.0
29	1176.0
30	1508.0
31	1799.0
32	2090.0
33	2090.0
34	2669.5
35	3249.0
36	3542.5
37	3836.0
38	4696.0
39	5556.0
40	5556.0
41	6912.0
42	8268.0
43	9624.5
44	10981.0
45	15090.5
46	19200.0
47	19200.0
48	21378.0
49	23556.0
50	25836.0
51	28116.0
52	28214.5
53	28313.0
54	28313.0
55	25522.5
56	22732.0
57	21315.5
58	19899.0
59	18373.0
60	16847.0
61	16847.0
62	15254.5
63	13662.0
64	11572.5
65	9483.0
66	8181.0
67	6879.0
68	6879.0
69	5861.5
70	4844.0
71	4059.5
72	3275.0
73	2671.5
74	2068.0
75	2068.0
76	1666.5
77	1265.0
78	1053.0
79	841.0
80	649.5
81	458.0
82	458.0
83	367.5
84	277.0
85	214.0
86	151.0
87	113.5
88	76.0
89	76.0
90	52.5
91	29.0
92	17.5
93	6.0
94	3.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	241963.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.18560047966486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.93748539752535	41.622675069482455
2	8.249547411161245	8.280169810291836
3	2.771609774507226	4.172847024868609
4	1.4041458678567262	2.818716141577198
5	0.8774495066513341	2.2017665190941447
6	0.562988570657596	1.6952351688983809
7	0.44466618965179294	1.5621087818475834
8	0.34850683128737836	1.3992019679537866
9	0.296006165283555	1.3369722435394318
>10	1.8234804359419068	17.697358798677648
>50	0.16730048976106213	5.822107055851814
>100	0.11582341598842763	11.133991059706032
>500	9.899437263968173E-4	0.2568503582110579
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	517	0.2136690320420891	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	485	0.20044386951724025	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	471	0.19465786091261889	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	448	0.18515227534788375	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	435	0.17977955307216392	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	425	0.17564668978314865	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	396	0.1636613862450044	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	376	0.15539565966697388	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.15374251435136777	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	368	0.15208936903576167	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	358	0.1479565057467464	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	357	0.14754321941784487	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	344	0.14217049714212504	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	336	0.13886420651091283	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	310	0.12811876195947314	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	308	0.1272921893016701	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	288	0.11902646272363955	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	281	0.11613345842132888	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	277	0.11448031310572278	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	275	0.11365374044791973	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	273	0.11282716779011667	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	269	0.11117402247451057	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	268	0.11076073614560904	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	263	0.10869430450110142	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	256	0.10580130019879072	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	253	0.10456144121208613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	4.1328632890152625E-4	0.0	0.0	0.0	0.0
10	4.1328632890152625E-4	0.0	0.0	0.0	0.0
11	4.1328632890152625E-4	0.0	0.0	0.0	0.0
12	4.1328632890152625E-4	0.0	0.0	0.0	0.0
13	4.1328632890152625E-4	0.0	0.0	0.0	0.0
14	4.1328632890152625E-4	0.0	0.0	4.1328632890152625E-4	0.0
15	4.1328632890152625E-4	0.0	0.0	8.265726578030525E-4	0.0
16	4.1328632890152625E-4	0.0	0.0	8.265726578030525E-4	0.0
17	4.1328632890152625E-4	0.0	0.0	8.265726578030525E-4	0.0
18	4.1328632890152625E-4	0.0	0.0	8.265726578030525E-4	0.0
19	4.1328632890152625E-4	0.0	0.0	0.0012398589867045789	0.0
20	4.1328632890152625E-4	0.0	0.0	0.0012398589867045789	0.0
21	4.1328632890152625E-4	0.0	0.0	0.0024797179734091577	0.0
22	4.1328632890152625E-4	0.0	0.0	0.004132863289015263	0.0
23	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.006199294933522894	0.0
24	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.009092299235833578	0.0
25	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.011572017209242735	0.0
26	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.01777131214276563	0.0
27	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.04339506453466026	0.0
28	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.1305984799328823	0.0
29	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.2864074259287577	0.0
30	4.1328632890152625E-4	4.1328632890152625E-4	0.0	0.4980100263263392	0.0
31	4.1328632890152625E-4	4.1328632890152625E-4	0.0	1.0815703227352942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTTAT	20	0.0018390659	37.0	1
ATACGGC	35	2.3805791E-5	31.714285	29
GGTATCA	315	0.0	30.539682	1
TTATCCT	25	0.0054878774	29.599998	4
TCAAGAT	25	0.0054878774	29.599998	13
AACTCCG	160	0.0	26.59375	5
CGTGCCA	185	0.0	25.0	10
CTTTGTT	45	1.3189987E-4	24.666666	9
GCGGTAA	175	0.0	24.314287	23
CTAACTC	175	0.0	24.314287	3
TCCGTGC	175	0.0	24.314287	8
CCGCGGT	175	0.0	24.314287	21
CGGTAAT	175	0.0	24.314287	24
TAACTCC	175	0.0	24.314287	4
GGTAATA	195	0.0	23.717949	25
CGCGGTA	180	0.0	23.63889	22
CCGTGCC	190	0.0	23.368422	9
ACAATAT	40	0.0019268076	23.125	35
TTAAACC	40	0.0019268076	23.125	4
TGTAGGC	40	0.0019268076	23.125	10
>>END_MODULE