FastQCFastQC Report
Fri 10 Feb 2017
ERR1631636.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631636.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234704
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA6840.2914309087190674No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6390.2722578226191288No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5570.23732019905924057No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC5430.23135523893925966No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5300.2258163473992774No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5260.22411207307928283No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5190.2211295930192924No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA5160.21985138727929646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5110.21772104437930329No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5040.21473856431931285No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4600.19599154679937283No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4560.19428727247937827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4400.1874701751994001No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4300.18320948939941373No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3350.14273297429954326No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3270.13932442565955416No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT3220.13719408275956096No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3220.13719408275956096No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3170.1350637398595678No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3150.13421160269957053No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3110.132507328379576No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3030.1290987797395869No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3030.1290987797395869No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT2990.12739450541959235No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2760.11759492807962368No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG2740.11674279091962643No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2700.11503851659963188No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2660.11333424227963733No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2640.11248210511964006No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2600.11077783079964552No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2550.10864748789965234No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2530.10779535073965506No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2520.10736928215965642No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2480.10566500783966187No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2450.10438680209966596No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2420.10310859635967004No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2360.10055218487967824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC352.380277E-531.7142853
GGTATCA4050.029.6913591
TAGGGAG250.005487546329.5999985
TAAAGAC905.456968E-1226.72222137
CAATCTG358.848294E-426.4285734
CTAAGTC509.050904E-625.89999834
AGAACAG601.329845E-624.6666665
TAGAACA701.9093932E-723.7857134
TAAGTCT551.8943074E-523.54545435
ATCTCGC400.001926636323.12511
AAATCTC400.001926636323.1259
AATGTTC400.001926636323.12520
CTTATAC2400.023.12499837
CCCGTAG502.6932344E-422.19999927
AACAGAG502.6932344E-422.1999997
GAAACAT1700.021.76470627
CTGTAGA603.710048E-521.5833321
TCCTACA603.710048E-521.5833322
AGAGAGA603.710048E-521.58333221
GTATCAA5600.021.1428572
Produced by FastQC (version 0.11.5)