##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631636.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234704
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59742058081669	34.0	31.0	34.0	31.0	34.0
2	32.76652720021815	34.0	31.0	34.0	31.0	34.0
3	32.853794566773466	34.0	31.0	34.0	31.0	34.0
4	36.280293476037905	37.0	37.0	37.0	35.0	37.0
5	36.20057178403436	37.0	35.0	37.0	35.0	37.0
6	36.25377496761879	37.0	37.0	37.0	35.0	37.0
7	36.247631058695205	37.0	37.0	37.0	35.0	37.0
8	36.23501516804145	37.0	37.0	37.0	35.0	37.0
9	38.05673955279842	39.0	38.0	39.0	35.0	39.0
10	37.99450797600382	39.0	38.0	39.0	35.0	39.0
11	38.06975594791738	39.0	38.0	39.0	37.0	39.0
12	38.00827851250937	39.0	38.0	39.0	35.0	39.0
13	38.051017451769034	39.0	38.0	39.0	35.0	39.0
14	39.430410218828825	40.0	39.0	41.0	37.0	41.0
15	39.45030762151476	40.0	39.0	41.0	37.0	41.0
16	39.39224299543255	40.0	39.0	41.0	37.0	41.0
17	39.36020263821665	40.0	39.0	41.0	36.0	41.0
18	39.39197031154134	40.0	39.0	41.0	37.0	41.0
19	39.4064055150317	40.0	39.0	41.0	37.0	41.0
20	39.4052593905515	40.0	39.0	41.0	37.0	41.0
21	39.37853210852819	40.0	39.0	41.0	36.0	41.0
22	39.35435697729907	40.0	39.0	41.0	36.0	41.0
23	39.28777097961688	40.0	39.0	41.0	36.0	41.0
24	39.29429835026246	40.0	39.0	41.0	36.0	41.0
25	39.25635694321358	40.0	39.0	41.0	36.0	41.0
26	39.187862158293	40.0	39.0	41.0	36.0	41.0
27	39.076185322789556	40.0	39.0	41.0	35.0	41.0
28	39.0626874701752	40.0	39.0	41.0	35.0	41.0
29	39.05206558047583	40.0	39.0	41.0	35.0	41.0
30	39.0010481287068	40.0	39.0	41.0	35.0	41.0
31	38.91615822482787	40.0	38.0	41.0	35.0	41.0
32	38.87295913150181	40.0	38.0	41.0	35.0	41.0
33	38.80209540527643	40.0	38.0	41.0	35.0	41.0
34	38.77056633035654	40.0	38.0	41.0	35.0	41.0
35	38.70869265116913	40.0	38.0	41.0	35.0	41.0
36	38.6576198104847	40.0	38.0	41.0	35.0	41.0
37	38.61912451428182	40.0	38.0	41.0	35.0	41.0
38	38.57452791601336	40.0	38.0	41.0	35.0	41.0
39	38.494196945940416	40.0	38.0	41.0	35.0	41.0
40	38.16496523280387	40.0	38.0	41.0	34.0	41.0
41	38.25421381825619	40.0	37.0	41.0	34.0	41.0
42	38.24099291021883	40.0	37.0	41.0	34.0	41.0
43	37.77601148680892	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	0.0
18	1.0
19	2.0
20	9.0
21	21.0
22	34.0
23	66.0
24	100.0
25	194.0
26	326.0
27	491.0
28	776.0
29	1132.0
30	1490.0
31	2142.0
32	3005.0
33	3976.0
34	6069.0
35	8783.0
36	13419.0
37	24541.0
38	56003.0
39	112121.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.10026245824528	16.816500783966188	13.5932919762765	27.489944781512033
2	20.392068307314744	19.903367646056306	33.24314881723362	26.461415229395325
3	20.95575703865294	19.956200149976137	29.898084395664327	29.18995841570659
4	15.194457699911379	16.826300361306156	34.42974981252983	33.54949212625264
5	16.288601813347878	33.20735905651374	33.992603449451224	16.511435680687164
6	32.469834344536096	34.18476037903061	16.440282227827392	16.9051230486059
7	29.407679460085895	28.25899856840957	20.5931726770741	21.74014929443043
8	26.6241734269548	30.900623764401118	21.18114731747222	21.29405549117186
9	28.158020314949894	13.28694866725748	18.366112209421228	40.18891880837139
10	19.09980230417888	24.022172608903126	29.88317199536437	26.994853091553615
11	38.03855068511828	19.95577408139614	19.371207989638012	22.63446724384757
12	22.00644215692958	24.49297838980162	27.174227963733045	26.326351489535753
13	34.68198241188902	18.00906673938237	21.51859363283114	25.79035721589747
14	23.444849683004975	21.120219510532415	24.041771763583068	31.393159042879542
15	29.404696980025907	24.484883086781647	20.812597995773398	25.297821937419045
16	24.286335128502284	25.002556411479993	23.395851796305134	27.31525666371259
17	26.292266003135868	24.350671484082078	22.559905242347806	26.797157270434248
18	26.114169336696435	21.929323743949826	23.947184538823368	28.00932238053037
19	27.426034494512237	22.885847706046768	24.22114663576249	25.466971163678505
20	29.30712727520622	21.13683618515236	23.76908787238394	25.78694866725748
21	26.712795691594522	22.76867884654714	22.736297634467245	27.782227827391097
22	27.746011998091213	23.297429954325448	22.30980298588861	26.646755061694734
23	27.230895084872863	22.706898902447335	23.3434453609653	26.7187606517145
24	27.040868498193472	22.308098711568615	24.247988956302407	26.40304383393551
25	27.29650964619265	22.476821869248074	24.12144658804281	26.10522189651646
26	27.00593087463358	23.44655395732497	23.431641557025017	26.115873611016426
27	26.090309496216513	22.27529143090872	25.299526211739042	26.33487286113573
28	25.541959233758266	23.526228781784717	23.011964005726362	27.919847978730655
29	26.613947781034835	24.079691867202946	23.111237984866044	26.19512236689617
30	26.082640261776536	22.792538687027065	24.34939327834208	26.77542777285432
31	27.44009475765219	22.901612243506715	23.660440384484286	25.99785261435681
32	25.086917990319723	22.80319040152703	23.82447678778376	28.285414820369486
33	25.210477878519328	22.282960665348696	25.60373917785807	26.902822278273913
34	25.46228440929852	22.707324971027337	24.660423341741087	27.169967277933054
35	25.092456881859704	24.229668007362466	25.310603994819008	25.367271115958822
36	25.48827459267844	23.02431999454632	25.537698547958282	25.949706864816964
37	26.170410389256254	22.775495943827117	25.10992569363965	25.944167973276976
38	25.20067830117936	22.119350330629217	25.87855341195719	26.801417956234236
39	24.714960119980912	20.992398936532826	27.020843274933533	27.271797668552733
40	24.58671347740132	22.31875042606858	27.410696025632287	25.683840070897812
41	22.76612243506715	21.599546663030882	28.480128161428865	27.154202740473103
42	21.592729565750904	21.808746335810213	29.054468607267026	27.54405549117186
43	20.856909128093257	22.954870816006544	28.80990524234781	27.37831481355239
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	2.5
2	1.0
3	10.5
4	20.0
5	20.0
6	32.0
7	44.0
8	40.5
9	37.0
10	73.5
11	110.0
12	110.0
13	221.0
14	332.0
15	577.0
16	822.0
17	789.0
18	756.0
19	756.0
20	846.0
21	936.0
22	783.5
23	631.0
24	659.5
25	688.0
26	688.0
27	755.5
28	823.0
29	1184.5
30	1546.0
31	1745.5
32	1945.0
33	1945.0
34	2466.5
35	2988.0
36	3324.0
37	3660.0
38	4710.5
39	5761.0
40	5761.0
41	7072.5
42	8384.0
43	9613.0
44	10842.0
45	15404.5
46	19967.0
47	19967.0
48	22087.5
49	24208.0
50	26900.5
51	29593.0
52	29434.5
53	29276.0
54	29276.0
55	25513.5
56	21751.0
57	20081.5
58	18412.0
59	16694.5
60	14977.0
61	14977.0
62	13633.5
63	12290.0
64	9955.5
65	7621.0
66	6605.0
67	5589.0
68	5589.0
69	4818.5
70	4048.0
71	3359.0
72	2670.0
73	2121.5
74	1573.0
75	1573.0
76	1271.5
77	970.0
78	799.0
79	628.0
80	484.5
81	341.0
82	341.0
83	284.5
84	228.0
85	165.5
86	103.0
87	90.0
88	77.0
89	77.0
90	58.5
91	40.0
92	24.5
93	9.0
94	6.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	234704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.43454640376463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.25009767523278	38.656805236185335
2	7.433689783286702	6.903600263864348
3	2.6594447362774334	3.7047033004476613
4	1.4018135971416312	2.603703141036051
5	0.8658345187587766	2.0102316569642813
6	0.6452742773482408	1.7977811024809542
7	0.5415344287474909	1.7602133892638074
8	0.36800190122989873	1.367040108746667
9	0.3061692728060895	1.2795148174969109
>10	2.1671194517216934	19.433523850616933
>50	0.22040193092896695	7.05284325730872
>100	0.13064548846920665	10.869621597869356
>500	0.009972938051084478	2.56041827771897
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	684	0.2914309087190674	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	639	0.2722578226191288	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	557	0.23732019905924057	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	543	0.23135523893925966	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	530	0.2258163473992774	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	526	0.22411207307928283	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	519	0.2211295930192924	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	516	0.21985138727929646	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	511	0.21772104437930329	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	504	0.21473856431931285	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	460	0.19599154679937283	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	456	0.19428727247937827	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	440	0.1874701751994001	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	430	0.18320948939941373	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	335	0.14273297429954326	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	327	0.13932442565955416	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	322	0.13719408275956096	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	322	0.13719408275956096	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	317	0.1350637398595678	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.13421160269957053	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	311	0.132507328379576	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	303	0.1290987797395869	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	303	0.1290987797395869	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	299	0.12739450541959235	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	276	0.11759492807962368	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	274	0.11674279091962643	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	270	0.11503851659963188	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	266	0.11333424227963733	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	264	0.11248210511964006	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	260	0.11077783079964552	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	255	0.10864748789965234	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	253	0.10779535073965506	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	252	0.10736928215965642	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	248	0.10566500783966187	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	245	0.10438680209966596	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	242	0.10310859635967004	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	236	0.10055218487967824	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	4.2606857999863656E-4	0.0
4	0.0	0.0	0.0	4.2606857999863656E-4	0.0
5	0.0	0.0	0.0	4.2606857999863656E-4	0.0
6	0.0	0.0	0.0	4.2606857999863656E-4	0.0
7	0.0	0.0	0.0	4.2606857999863656E-4	0.0
8	0.0	0.0	0.0	4.2606857999863656E-4	0.0
9	0.0	0.0	0.0	4.2606857999863656E-4	0.0
10	0.0	0.0	0.0	4.2606857999863656E-4	0.0
11	0.0	0.0	0.0	4.2606857999863656E-4	0.0
12	0.0	0.0	0.0	4.2606857999863656E-4	0.0
13	0.0	0.0	0.0	4.2606857999863656E-4	0.0
14	0.0	0.0	0.0	4.2606857999863656E-4	0.0
15	0.0	0.0	0.0	4.2606857999863656E-4	0.0
16	0.0	0.0	0.0	0.0017042743199945462	0.0
17	0.0	0.0	0.0	0.0021303428999931828	0.0
18	0.0	0.0	0.0	0.0021303428999931828	0.0
19	0.0	0.0	0.0	0.0021303428999931828	0.0
20	0.0	0.0	0.0	0.0034085486399890925	0.0
21	0.0	0.0	0.0	0.008095303019974095	0.0
22	0.0	0.0	0.0	0.010651714499965914	0.0
23	0.0	0.0	0.0	0.01235598881996046	0.0
24	0.0	0.0	0.0	0.014912400299952281	0.0
25	0.0	0.0	0.0	0.014912400299952281	0.0
26	0.0	0.0	0.0	0.017894880359942737	0.0
27	4.2606857999863656E-4	0.0	0.0	0.034511554979889565	0.0
28	4.2606857999863656E-4	0.0	0.0	0.1141863794396346	0.0
29	4.2606857999863656E-4	0.0	0.0	0.2398766105392324	0.0
30	4.2606857999863656E-4	0.0	0.0	0.4171211398186652	0.0
31	4.2606857999863656E-4	0.0	0.0	0.949706864816961	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGAAC	35	2.380277E-5	31.714285	3
GGTATCA	405	0.0	29.691359	1
TAGGGAG	25	0.0054875463	29.599998	5
TAAAGAC	90	5.456968E-12	26.722221	37
CAATCTG	35	8.848294E-4	26.42857	34
CTAAGTC	50	9.050904E-6	25.899998	34
AGAACAG	60	1.329845E-6	24.666666	5
TAGAACA	70	1.9093932E-7	23.785713	4
TAAGTCT	55	1.8943074E-5	23.545454	35
ATCTCGC	40	0.0019266363	23.125	11
AAATCTC	40	0.0019266363	23.125	9
AATGTTC	40	0.0019266363	23.125	20
CTTATAC	240	0.0	23.124998	37
CCCGTAG	50	2.6932344E-4	22.199999	27
AACAGAG	50	2.6932344E-4	22.199999	7
GAAACAT	170	0.0	21.764706	27
CTGTAGA	60	3.710048E-5	21.583332	1
TCCTACA	60	3.710048E-5	21.583332	2
AGAGAGA	60	3.710048E-5	21.583332	21
GTATCAA	560	0.0	21.142857	2
>>END_MODULE