FastQCFastQC Report
Fri 10 Feb 2017
ERR1631635.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631635.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230560
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA7820.3391741845940319No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA6980.30274115197779317No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC6170.26760929909784875No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA5540.24028452463566966No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG5380.2333448993754337No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA5260.2281401804302568No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT5190.22510409437890352No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC5010.21729701596113812No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA4850.21035739070090215No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA4660.20211658570437196No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT4630.2008154059680777No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4270.18520124913254685No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3580.15527411519777934No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA3460.15006939625260235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.14529840388619014No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT3340.1448646773074254No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA3300.1431297709923664No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA3290.14269604441360167No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA2870.1244795281054823No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2750.11927480916030535No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2740.11884108258154061No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT2690.11667244968771685No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2690.11667244968771685No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2670.11580499653018737No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC2620.11363636363636363No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG2590.11233518390006939No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2550.1106002775850104No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA2530.10973282442748092No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA2460.1066967383761277No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2430.10539555863983345No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2420.10496183206106871No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC2410.10452810548230394No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2380.10322692574600972No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2360.10235947258848022No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2340.10149201943095074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCTA200.00183888637.04
GCGTAGT352.380095E-531.71428733
GGTATCA4100.030.2317091
AGGCGTA405.9198665E-527.75000231
ATACACA509.049994E-625.90000237
CGAAAGG451.3187349E-424.66666627
CACAATA400.001926533123.12500234
TCACAAT502.6930333E-422.233
GGGCAGT1151.3278623E-1020.9130447
CAGTCGG1151.3278623E-1020.91304410
AGTCGGT1151.3278623E-1020.91304411
ACTTGCA450.003816676320.5555552
CGGTTGA450.003816676320.55555519
TAGAACA450.003816676320.5555554
GCCATGA450.003816676320.55555533
TAACCCT450.003816676320.55555535
CTTATAC2250.020.55555537
TCTCGGT450.003816676320.55555516
GTCAATT450.003816676320.5555552
GTAGTCG450.003816676320.55555535
Produced by FastQC (version 0.11.5)