##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631635.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230560
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6064972241499	34.0	31.0	34.0	31.0	34.0
2	32.77149982650937	34.0	31.0	34.0	31.0	34.0
3	32.85441099930604	34.0	31.0	34.0	31.0	34.0
4	36.28754337265788	37.0	37.0	37.0	35.0	37.0
5	36.20762925052047	37.0	35.0	37.0	35.0	37.0
6	36.251721894517694	37.0	37.0	37.0	35.0	37.0
7	36.25078504510756	37.0	37.0	37.0	35.0	37.0
8	36.22704718945177	37.0	37.0	37.0	35.0	37.0
9	38.06113376127689	39.0	38.0	39.0	35.0	39.0
10	37.99251821651631	39.0	38.0	39.0	35.0	39.0
11	38.06303782095767	39.0	38.0	39.0	35.0	39.0
12	37.99215822345593	39.0	38.0	39.0	35.0	39.0
13	38.05232043719639	39.0	38.0	39.0	35.0	39.0
14	39.421803435114505	40.0	39.0	41.0	37.0	41.0
15	39.43992019430951	40.0	39.0	41.0	37.0	41.0
16	39.37843077723803	40.0	39.0	41.0	36.0	41.0
17	39.35699167244969	40.0	39.0	41.0	36.0	41.0
18	39.38063844552394	40.0	39.0	41.0	36.0	41.0
19	39.40767262317835	40.0	39.0	41.0	37.0	41.0
20	39.40739503816794	40.0	39.0	41.0	37.0	41.0
21	39.3661519777932	40.0	39.0	41.0	36.0	41.0
22	39.337686502428866	40.0	39.0	41.0	36.0	41.0
23	39.27168632893824	40.0	39.0	41.0	36.0	41.0
24	39.28994621790423	40.0	39.0	41.0	36.0	41.0
25	39.23340562109646	40.0	39.0	41.0	36.0	41.0
26	39.16631679389313	40.0	39.0	41.0	36.0	41.0
27	39.0573039555864	40.0	39.0	41.0	35.0	41.0
28	39.050611554476056	40.0	39.0	41.0	35.0	41.0
29	39.02841342817488	40.0	39.0	41.0	35.0	41.0
30	38.98547015961138	40.0	39.0	41.0	35.0	41.0
31	38.89803088133241	40.0	38.0	41.0	35.0	41.0
32	38.85337439278279	40.0	38.0	41.0	35.0	41.0
33	38.79154233171409	40.0	38.0	41.0	35.0	41.0
34	38.76826856349757	40.0	38.0	41.0	35.0	41.0
35	38.68532702984039	40.0	38.0	41.0	35.0	41.0
36	38.644478660652325	40.0	38.0	41.0	35.0	41.0
37	38.59906315058987	40.0	38.0	41.0	35.0	41.0
38	38.56107737682165	40.0	38.0	41.0	35.0	41.0
39	38.49328157529494	40.0	38.0	41.0	34.0	41.0
40	38.17450555170021	40.0	38.0	41.0	34.0	41.0
41	38.25822779319917	40.0	37.0	41.0	34.0	41.0
42	38.235851839000695	40.0	37.0	41.0	34.0	41.0
43	37.77273160999306	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	11.0
21	21.0
22	27.0
23	67.0
24	120.0
25	179.0
26	311.0
27	488.0
28	791.0
29	1030.0
30	1536.0
31	2157.0
32	2864.0
33	3984.0
34	5851.0
35	8857.0
36	13466.0
37	24141.0
38	55060.0
39	109589.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.89191533657183	16.64555863983345	13.436849410131854	28.025676613462874
2	20.458015267175572	19.685981956974324	32.724670367800144	27.131332408049964
3	20.78027411519778	19.65909090909091	29.990458015267173	29.570176960444133
4	15.189538514920194	16.558379597501734	34.4955759888966	33.756505898681475
5	16.476839000693964	32.967557251908396	33.75260235947259	16.80300138792505
6	32.44968771686329	34.20671408743928	16.47467036780014	16.86892782789729
7	29.496009715475363	28.08379597501735	20.747744621790424	21.672449687716863
8	26.91360166551006	30.71087786259542	21.02532963219986	21.35019083969466
9	28.676266481609993	12.825294934073561	18.22562456627342	40.27281401804302
10	19.19890700902151	23.536606523247745	29.67557251908397	27.58891394864677
11	38.27810548230396	19.869882026370576	19.027151283830673	22.824861207494795
12	22.150416377515615	24.45523941707148	26.986034004163773	26.40831020124913
13	35.06982997918112	18.011363636363637	21.16065232477446	25.758154059680777
14	23.444222761970853	21.181471200555173	23.943008327550313	31.431297709923665
15	29.57321304649549	24.43095072866065	20.478400416377514	25.51743580846634
16	24.462179042331712	24.757546842470507	23.269430950728662	27.51084316446912
17	26.284264399722414	24.56887578070784	22.155187369882025	26.991672449687716
18	26.283396946564885	21.57009021512838	23.780360860513532	28.366151977793198
19	27.45402498265094	22.820957668285914	23.941707147814018	25.783310201249133
20	30.040336571825122	20.731263011797363	23.478487161693266	25.749913254684248
21	26.937456627342122	22.624479528105482	22.342990978487162	28.095072866065234
22	28.300225537820957	23.164902845246356	21.88887925052047	26.645992366412212
23	27.517349063150593	22.458362248438586	22.975798056904928	27.0484906315059
24	27.13436849410132	21.870229007633586	23.90267175572519	27.092730742539906
25	27.22111380985427	22.314365024288687	24.005031228313673	26.459489937543374
26	26.916204024982648	23.51318528799445	23.01006245662734	26.560548230395558
27	26.26734906315059	22.082755031228313	25.330933379597504	26.318962526023594
28	25.568615544760583	23.424271339347673	22.66004510756419	28.347068008327554
29	26.655968077723802	23.9000693962526	22.987942401110338	26.456020124913255
30	26.378383067314363	22.263619014573212	24.295194309507288	27.062803608605137
31	27.54467383761277	22.771946564885496	23.34099583622484	26.34238376127689
32	25.287126995142263	22.686936155447608	23.271599583622486	28.75433726578765
33	25.114937543372655	22.12786259541985	25.60634975711312	27.150850104094378
34	25.7911172796669	22.21937890353921	24.659090909090907	27.330412907702982
35	24.882460097154755	24.189798750867453	25.419847328244277	25.507893823733518
36	25.54302567661346	22.741585704371964	25.721287300485773	25.994101318528802
37	26.105569049271338	22.613636363636363	25.140527411519777	26.140267175572518
38	25.49227966689799	21.839000693962525	25.780274115197777	26.888445523941705
39	25.124479528105482	20.602879944483	26.97909437890354	27.29354614850798
40	24.64824774462179	21.90709576682859	27.692140874392784	25.752515614156835
41	22.744621790423317	21.052654406662043	28.8632026370576	27.339521165857043
42	21.355829285218597	21.395732130464957	29.614850798056906	27.633587786259543
43	20.414208882720335	22.423664122137403	29.375	27.787126995142263
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4.0
1	3.0
2	2.0
3	9.0
4	16.0
5	16.0
6	22.5
7	29.0
8	30.5
9	32.0
10	50.0
11	68.0
12	68.0
13	136.5
14	205.0
15	430.5
16	656.0
17	634.5
18	613.0
19	613.0
20	697.5
21	782.0
22	666.5
23	551.0
24	562.0
25	573.0
26	573.0
27	665.0
28	757.0
29	1035.0
30	1313.0
31	1540.0
32	1767.0
33	1767.0
34	2243.5
35	2720.0
36	3119.5
37	3519.0
38	4392.5
39	5266.0
40	5266.0
41	6300.0
42	7334.0
43	8696.0
44	10058.0
45	14640.0
46	19222.0
47	19222.0
48	21370.0
49	23518.0
50	26431.5
51	29345.0
52	29376.5
53	29408.0
54	29408.0
55	25915.0
56	22422.0
57	20770.5
58	19119.0
59	17370.0
60	15621.0
61	15621.0
62	14004.0
63	12387.0
64	9955.0
65	7523.0
66	6528.0
67	5533.0
68	5533.0
69	4662.5
70	3792.0
71	3228.5
72	2665.0
73	2039.0
74	1413.0
75	1413.0
76	1161.5
77	910.0
78	749.0
79	588.0
80	482.5
81	377.0
82	377.0
83	289.0
84	201.0
85	163.5
86	126.0
87	101.5
88	77.0
89	77.0
90	58.5
91	40.0
92	24.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	230560.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.21721671842201
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.288349183509	39.32644033517351
2	7.3232733959964325	6.915691740540357
3	2.6749697800238916	3.7891388345585315
4	1.3899085838737673	2.6251045929425074
5	0.9876461908033873	2.331695211614375
6	0.6702960219602885	1.898970752063706
7	0.5017101741142828	1.6582550614693925
8	0.40595423992107704	1.533442345929262
9	0.2972340298062289	1.2631107241305557
>10	2.1255221135629347	19.37428723541593
>50	0.21045760844185715	6.726699008506079
>100	0.11669924259572173	10.327176073550467
>500	0.00797943539116046	2.22998808410532
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	782	0.3391741845940319	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	698	0.30274115197779317	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	617	0.26760929909784875	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	554	0.24028452463566966	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	538	0.2333448993754337	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	526	0.2281401804302568	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	519	0.22510409437890352	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	501	0.21729701596113812	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	485	0.21035739070090215	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	466	0.20211658570437196	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	463	0.2008154059680777	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	427	0.18520124913254685	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.15527411519777934	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	346	0.15006939625260235	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	335	0.14529840388619014	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	334	0.1448646773074254	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	330	0.1431297709923664	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	329	0.14269604441360167	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	287	0.1244795281054823	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.11927480916030535	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	274	0.11884108258154061	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	269	0.11667244968771685	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	269	0.11667244968771685	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	267	0.11580499653018737	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	262	0.11363636363636363	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	259	0.11233518390006939	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	255	0.1106002775850104	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	253	0.10973282442748092	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	246	0.1066967383761277	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	243	0.10539555863983345	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	242	0.10496183206106871	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	241	0.10452810548230394	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	238	0.10322692574600972	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	236	0.10235947258848022	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	234	0.10149201943095074	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	4.337265787647467E-4	0.0	0.0	0.0	0.0
3	4.337265787647467E-4	0.0	0.0	0.0	0.0
4	4.337265787647467E-4	0.0	0.0	0.0	0.0
5	4.337265787647467E-4	0.0	0.0	0.0	0.0
6	4.337265787647467E-4	0.0	0.0	0.0	0.0
7	4.337265787647467E-4	0.0	0.0	0.0	0.0
8	4.337265787647467E-4	0.0	0.0	0.0	0.0
9	4.337265787647467E-4	0.0	0.0	0.0	0.0
10	4.337265787647467E-4	0.0	0.0	0.0	0.0
11	4.337265787647467E-4	0.0	0.0	0.0	0.0
12	4.337265787647467E-4	0.0	0.0	0.0	0.0
13	4.337265787647467E-4	0.0	0.0	0.0	0.0
14	4.337265787647467E-4	0.0	0.0	4.337265787647467E-4	0.0
15	4.337265787647467E-4	0.0	0.0	0.0013011797362942402	0.0
16	4.337265787647467E-4	0.0	0.0	0.0013011797362942402	0.0
17	8.674531575294934E-4	0.0	0.0	0.0026023594725884803	0.0
18	8.674531575294934E-4	0.0	0.0	0.003036086051353227	0.0
19	8.674531575294934E-4	0.0	0.0	0.004337265787647467	0.0
20	8.674531575294934E-4	0.0	0.0	0.006939625260235947	0.0
21	0.0013011797362942402	0.0	0.0	0.008240804996530187	0.0
22	0.0013011797362942402	0.0	0.0	0.010409437890353921	0.0
23	0.0013011797362942402	0.0	0.0	0.012578070784177654	0.0
24	0.0013011797362942402	0.0	0.0	0.01431297709923664	0.0
25	0.0013011797362942402	0.0	0.0	0.015180430256766134	0.0
26	0.0013011797362942402	0.0	0.0	0.020818875780707843	0.0
27	0.0013011797362942402	0.0	0.0	0.04380638445523942	0.0
28	0.0013011797362942402	0.0	0.0	0.13272033310201248	0.0
29	0.0013011797362942402	0.0	0.0	0.2862595419847328	0.0
30	0.0013011797362942402	0.0	0.0	0.48837612768910477	0.0
31	0.0013011797362942402	0.0	0.0	1.0808466342817489	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCTA	20	0.001838886	37.0	4
GCGTAGT	35	2.380095E-5	31.714287	33
GGTATCA	410	0.0	30.231709	1
AGGCGTA	40	5.9198665E-5	27.750002	31
ATACACA	50	9.049994E-6	25.900002	37
CGAAAGG	45	1.3187349E-4	24.666666	27
CACAATA	40	0.0019265331	23.125002	34
TCACAAT	50	2.6930333E-4	22.2	33
GGGCAGT	115	1.3278623E-10	20.913044	7
CAGTCGG	115	1.3278623E-10	20.913044	10
AGTCGGT	115	1.3278623E-10	20.913044	11
ACTTGCA	45	0.0038166763	20.555555	2
CGGTTGA	45	0.0038166763	20.555555	19
TAGAACA	45	0.0038166763	20.555555	4
GCCATGA	45	0.0038166763	20.555555	33
TAACCCT	45	0.0038166763	20.555555	35
CTTATAC	225	0.0	20.555555	37
TCTCGGT	45	0.0038166763	20.555555	16
GTCAATT	45	0.0038166763	20.555555	2
GTAGTCG	45	0.0038166763	20.555555	35
>>END_MODULE