FastQCFastQC Report
Fri 10 Feb 2017
ERR1631634.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631634.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574009
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.21724398049507934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11250.19598995834560085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10700.1864082270487048No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10430.1817044680484104No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA7820.13623479771223101No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA7810.13606058441592378No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA7460.12996311904517177No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC5990.10435376448801326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7050.032.2765961
TAAGGTA303.5972538E-430.8333325
TAAGAGT250.005494080529.5999984
ATACGGC551.9005083E-523.54545429
ATAGCGA400.001930016123.12518
CTAAGTC400.001930016123.12534
CTACCGT400.001930016123.1258
GTAACGT2350.022.04255526
TGCGGGT2400.021.58333221
TTAGCTA705.0945837E-621.1428574
CTCATAT450.003823510420.5555552
GTGTAAA450.003823510420.5555551
ACCGTTT450.003823510420.55555510
ATTGACT450.003823510420.55555531
TAGCTCA450.003823510420.5555554
GGTAACG2550.020.31372525
GCGGGTA2550.020.31372522
CTTATAC5850.019.60683837
GCAGTCG851.2430864E-619.5882369
GGGTAAC2650.019.5471724
Produced by FastQC (version 0.11.5)