##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631634.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574009
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.6506866617074	34.0	31.0	34.0	31.0	34.0
2	32.81259875716234	34.0	31.0	34.0	31.0	34.0
3	32.89825595069067	34.0	31.0	34.0	31.0	34.0
4	36.32681543320749	37.0	37.0	37.0	35.0	37.0
5	36.24610589729429	37.0	37.0	37.0	35.0	37.0
6	36.29295882120315	37.0	37.0	37.0	35.0	37.0
7	36.27991895597456	37.0	37.0	37.0	35.0	37.0
8	36.26299239210535	37.0	37.0	37.0	35.0	37.0
9	38.09591138814896	39.0	38.0	39.0	37.0	39.0
10	38.03534265142184	39.0	38.0	39.0	35.0	39.0
11	38.103433918283514	39.0	38.0	39.0	37.0	39.0
12	38.04603063715029	39.0	38.0	39.0	35.0	39.0
13	38.089512533775604	39.0	38.0	39.0	37.0	39.0
14	39.50012107824093	41.0	39.0	41.0	37.0	41.0
15	39.506507737683556	41.0	39.0	41.0	37.0	41.0
16	39.4582628495372	41.0	39.0	41.0	37.0	41.0
17	39.43803668583594	41.0	39.0	41.0	37.0	41.0
18	39.454165352808054	41.0	39.0	41.0	37.0	41.0
19	39.48159523631163	41.0	39.0	41.0	37.0	41.0
20	39.47352741855964	41.0	39.0	41.0	37.0	41.0
21	39.4397840451979	41.0	39.0	41.0	37.0	41.0
22	39.41928262448847	41.0	39.0	41.0	37.0	41.0
23	39.34593011607832	41.0	39.0	41.0	36.0	41.0
24	39.35795954418833	41.0	39.0	41.0	36.0	41.0
25	39.30533493377281	41.0	39.0	41.0	36.0	41.0
26	39.24054500887617	40.0	39.0	41.0	36.0	41.0
27	39.13580971726924	40.0	39.0	41.0	36.0	41.0
28	39.12490570705337	40.0	39.0	41.0	35.0	41.0
29	39.10451055645469	40.0	39.0	41.0	35.0	41.0
30	39.04999573177424	40.0	39.0	41.0	35.0	41.0
31	38.96032117963307	40.0	38.0	41.0	35.0	41.0
32	38.898153164845844	40.0	38.0	41.0	35.0	41.0
33	38.847498906811566	40.0	38.0	41.0	35.0	41.0
34	38.805370647498556	40.0	38.0	41.0	35.0	41.0
35	38.73307909806292	40.0	38.0	41.0	35.0	41.0
36	38.6753361010019	40.0	38.0	41.0	35.0	41.0
37	38.63587678938832	40.0	38.0	41.0	35.0	41.0
38	38.58548907769739	40.0	38.0	41.0	35.0	41.0
39	38.52070960559852	40.0	38.0	41.0	35.0	41.0
40	38.195998669010415	40.0	38.0	41.0	34.0	41.0
41	38.27235809891483	40.0	37.0	41.0	34.0	41.0
42	38.24459895228124	40.0	37.0	41.0	34.0	41.0
43	37.78699462900408	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	5.0
19	16.0
20	22.0
21	49.0
22	93.0
23	169.0
24	261.0
25	467.0
26	724.0
27	1104.0
28	1711.0
29	2460.0
30	3750.0
31	5005.0
32	6834.0
33	9594.0
34	13797.0
35	21286.0
36	32835.0
37	60529.0
38	131080.0
39	282215.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.06395892747326	17.230217644671075	12.992653425294726	26.713170002560936
2	19.849340341353532	20.201076986597773	34.190230466769684	25.75935220527901
3	20.34236396990291	20.948277814459352	29.94378136928167	28.765576846356065
4	15.129902144391464	16.160548005344864	34.03535484635258	34.67419500391109
5	16.155844246344568	34.22559576592005	33.42212404335124	16.196435944384145
6	33.74250229526018	34.389356264448814	15.714039326909509	16.154102113381498
7	29.972178136579743	29.038394868373146	20.137140706853028	20.852286288194087
8	26.86769719638542	32.58990712689174	20.00020905595557	20.542186620767268
9	27.370302556231696	13.976784336134102	18.04762643094446	40.60528667668974
10	18.810506455473693	24.675048649062994	30.560496438209157	25.953948457254157
11	37.57205897468507	20.80995245719144	20.117454604370312	21.50053396375318
12	22.46271399925785	24.295089449816988	27.60043832065351	25.64175823027165
13	33.363762589088324	18.397098303336705	22.62664871108293	25.612490396492042
14	23.590396666254364	20.27163337160219	24.167739530216426	31.97023043192703
15	27.919422866191994	25.326606377251927	21.261339107923394	25.492631648632685
16	25.07870085660678	24.809192887219538	23.054864993406028	27.057241262767658
17	25.83548341576526	24.629578978726816	23.052600220554034	26.482337384953897
18	25.638099751049197	22.760096096054244	24.48114925027308	27.120654902623475
19	27.4767468802754	23.274547960049407	24.33602957444918	24.912675585226015
20	28.137712126464915	22.277699478579606	24.215125546811983	25.3694628481435
21	26.47798205254621	23.622451912774885	23.131518843781194	26.768047190897704
22	26.756026473452504	23.950669763017654	22.56741619033848	26.725887573191358
23	26.462477069174874	23.49771519261893	23.737084261745025	26.30272347646117
24	26.829892911086763	22.823335522613757	23.73534212878195	26.61142943751753
25	26.935291955352614	23.29249192956905	24.03986697072694	25.732349144351396
26	26.805328836307446	23.470712131691315	23.858510929271144	25.8654481027301
27	26.43286080880265	22.96688727877089	24.52714156049818	26.073110351928282
28	25.592978507305634	24.216345039886132	23.708687494446952	26.48198895836128
29	26.033389720370238	24.521392521720042	23.478377516728834	25.96684024118089
30	25.533223346672262	24.4767939178654	24.356238316820818	25.63374441864152
31	26.41996902487592	23.439527951652327	23.90310953312579	26.237393490345973
32	24.198052643773877	23.790567743711335	24.455714109012227	27.555665503502556
33	24.9534414965619	23.073680029407203	25.535139692931647	26.437738781099252
34	25.050826729197627	22.973855810623178	25.377476659773627	26.59784080040557
35	25.183577260983714	23.989693541390466	25.418416784405817	25.408312413220003
36	24.94734403119115	23.33656789353477	25.94123088662373	25.77485718865035
37	25.545069850821157	22.499298791482364	25.33444597558575	26.621185382110735
38	24.306935953965876	22.325085495175163	26.62014010233289	26.747838448526068
39	23.87297063286464	21.47004663689942	27.781271722220385	26.87571100801555
40	23.82506197638016	22.166725608831918	28.31088014299427	25.69733227179365
41	22.802778353649508	21.5808462933508	29.23455904001505	26.38181631298464
42	21.993557592302558	21.739380392990356	29.53769017558958	26.729371839117505
43	20.935211817236315	22.19024440383339	29.634726981632692	27.239816797297607
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11.0
1	12.5
2	14.0
3	47.5
4	81.0
5	81.0
6	89.0
7	97.0
8	119.0
9	141.0
10	206.0
11	271.0
12	271.0
13	515.5
14	760.0
15	1562.5
16	2365.0
17	2384.5
18	2404.0
19	2404.0
20	2601.5
21	2799.0
22	2648.5
23	2498.0
24	2767.5
25	3037.0
26	3037.0
27	3438.5
28	3840.0
29	5371.0
30	6902.0
31	7810.0
32	8718.0
33	8718.0
34	10428.5
35	12139.0
36	13135.0
37	14131.0
38	16921.5
39	19712.0
40	19712.0
41	22878.0
42	26044.0
43	27904.5
44	29765.0
45	36490.0
46	43215.0
47	43215.0
48	47186.0
49	51157.0
50	54452.0
51	57747.0
52	59351.5
53	60956.0
54	60956.0
55	54933.0
56	48910.0
57	45371.5
58	41833.0
59	38075.0
60	34317.0
61	34317.0
62	31499.5
63	28682.0
64	24645.0
65	20608.0
66	18303.5
67	15999.0
68	15999.0
69	14017.5
70	12036.0
71	10468.5
72	8901.0
73	7170.5
74	5440.0
75	5440.0
76	4481.0
77	3522.0
78	2927.5
79	2333.0
80	1784.0
81	1235.0
82	1235.0
83	970.0
84	705.0
85	547.5
86	390.0
87	294.5
88	199.0
89	199.0
90	140.5
91	82.0
92	46.5
93	11.0
94	6.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	574009.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.97581391843932
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30192303208739	47.99494275333948
2	6.295046033398615	6.921505586802636
3	1.977851405991686	3.262019725623676
4	1.0013820517099021	2.2020717334427427
5	0.6542521404745726	1.7984021965235364
6	0.42655314217982954	1.407006370248234
7	0.30159453236666334	1.1606283423145887
8	0.22667200173720237	0.9969182230419674
9	0.18472391899695095	0.9139813017355112
>10	1.3548730572682537	14.70866754746801
>50	0.1722738640909046	6.601967190909486
>100	0.09807828008362969	10.089567610054841
>500	0.003502795717272488	1.1571649009692626
>1k	0.0012737438971899958	0.7851565175260428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1247	0.21724398049507934	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1125	0.19598995834560085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1070	0.1864082270487048	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1043	0.1817044680484104	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	782	0.13623479771223101	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	781	0.13606058441592378	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	746	0.12996311904517177	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	599	0.10435376448801326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	1.7421329630720075E-4	0.0	0.0	0.0	0.0
11	1.7421329630720075E-4	0.0	0.0	0.0	0.0
12	1.7421329630720075E-4	0.0	0.0	1.7421329630720075E-4	0.0
13	1.7421329630720075E-4	1.7421329630720075E-4	0.0	3.484265926144015E-4	0.0
14	1.7421329630720075E-4	1.7421329630720075E-4	0.0	3.484265926144015E-4	1.7421329630720075E-4
15	1.7421329630720075E-4	1.7421329630720075E-4	0.0	6.96853185228803E-4	1.7421329630720075E-4
16	1.7421329630720075E-4	1.7421329630720075E-4	0.0	0.0010452797778432046	1.7421329630720075E-4
17	1.7421329630720075E-4	1.7421329630720075E-4	0.0	0.001393706370457606	1.7421329630720075E-4
18	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.0015679196667648069	1.7421329630720075E-4
19	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.0020905595556864093	1.7421329630720075E-4
20	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.002787412740915212	1.7421329630720075E-4
21	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.003658479222451216	1.7421329630720075E-4
22	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.005749038778137625	1.7421329630720075E-4
23	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.006620105259673629	1.7421329630720075E-4
24	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.008710664815360038	1.7421329630720075E-4
25	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.00923330470428164	1.7421329630720075E-4
26	3.484265926144015E-4	1.7421329630720075E-4	0.0	0.01132386425996805	1.7421329630720075E-4
27	5.226398889216023E-4	1.7421329630720075E-4	0.0	0.028396767298073725	1.7421329630720075E-4
28	5.226398889216023E-4	1.7421329630720075E-4	0.0	0.11829082819258932	1.7421329630720075E-4
29	5.226398889216023E-4	1.7421329630720075E-4	0.0	0.2527834929417483	1.7421329630720075E-4
30	5.226398889216023E-4	1.7421329630720075E-4	0.0	0.4395401465830675	1.7421329630720075E-4
31	5.226398889216023E-4	1.7421329630720075E-4	0.0	1.0609589745108525	1.7421329630720075E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	705	0.0	32.276596	1
TAAGGTA	30	3.5972538E-4	30.833332	5
TAAGAGT	25	0.0054940805	29.599998	4
ATACGGC	55	1.9005083E-5	23.545454	29
ATAGCGA	40	0.0019300161	23.125	18
CTAAGTC	40	0.0019300161	23.125	34
CTACCGT	40	0.0019300161	23.125	8
GTAACGT	235	0.0	22.042555	26
TGCGGGT	240	0.0	21.583332	21
TTAGCTA	70	5.0945837E-6	21.142857	4
CTCATAT	45	0.0038235104	20.555555	2
GTGTAAA	45	0.0038235104	20.555555	1
ACCGTTT	45	0.0038235104	20.555555	10
ATTGACT	45	0.0038235104	20.555555	31
TAGCTCA	45	0.0038235104	20.555555	4
GGTAACG	255	0.0	20.313725	25
GCGGGTA	255	0.0	20.313725	22
CTTATAC	585	0.0	19.606838	37
GCAGTCG	85	1.2430864E-6	19.588236	9
GGGTAAC	265	0.0	19.54717	24
>>END_MODULE