FastQCFastQC Report
Fri 10 Feb 2017
ERR1631633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61955
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA2300.37123718828181745No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA1930.31151642321039463No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC1900.3066741990154144No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA1890.30506012428375434No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA1860.3002179000887741No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG1810.2921475264304737No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA1790.2889193769671536No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT1470.23726898555403117No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT1380.22274231296909047No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA1380.22274231296909047No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1380.22274231296909047No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC1300.20982971511580983No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT1280.2066015656524897No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA1210.1953030425308692No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA1060.17109192155596803No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1060.17109192155596803No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA1050.16947784682430797No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA980.15817932370268745No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG940.15172302477604713No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG940.15172302477604713No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT930.15010895004438704No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC930.15010895004438704No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT920.14849487531272698No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT910.1468808005810669No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC890.14365265111774675No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA880.1420385763860867No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT860.13881042692276652No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG860.13881042692276652No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA860.13881042692276652No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT850.13719635219110643No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC850.13719635219110643No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC840.13558227745944637No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT840.13558227745944637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT830.13396820272778628No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC830.13396820272778628No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA830.13396820272778628No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT820.13235412799612623No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG820.13235412799612623No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT790.127511903801146No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC770.12428375433782585No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC770.12428375433782585No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA770.12428375433782585No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC770.12428375433782585No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA750.12105560487450569No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC750.12105560487450569No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG750.12105560487450569No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC740.11944153014284561No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA740.11944153014284561No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA730.11782745541118553No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG730.11782745541118553No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC730.11782745541118553No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC720.11621338067952545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT720.11621338067952545No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC710.11459930594786538No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT700.11298523121620531No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA690.11137115648454524No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA670.10814300702122509No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC660.106528932289565No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG640.10330078282624484No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG640.10330078282624484No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA640.10330078282624484No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC630.10168670809458477No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC620.1000726333629247No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA620.1000726333629247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1300.031.3076921
TAAACCA303.560655E-430.8333345
ACGTGTC601.2205419E-930.8333348
CCACGTG551.7966158E-830.2727286
ACCACGT551.7966158E-830.2727285
TACCACG551.7966158E-830.2727284
AAACCAT250.005456791729.5999986
ACCATGC250.005456791729.5999988
GCTAAAC250.005456791729.5999983
GTGTCCC604.1856765E-827.75000210
CGTGTCC604.1856765E-827.7500029
CTAAACC405.8512785E-527.754
CGGTGCT358.7746326E-426.4285729
TGCGGGT358.7746326E-426.42857221
GGTGCTT358.7746326E-426.42857210
GTGCTTC358.7746326E-426.42857211
GGATACC659.095493E-825.6153851
GCCCTAC701.8625906E-723.78571517
CGCCCTA701.8625906E-723.78571516
CCGCCCT701.8625906E-723.78571515
Produced by FastQC (version 0.11.5)