##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631633.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61955
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58484383826971	34.0	31.0	34.0	31.0	34.0
2	32.759163909289	34.0	31.0	34.0	31.0	34.0
3	32.83389556936486	34.0	31.0	34.0	31.0	34.0
4	36.2416108465822	37.0	37.0	37.0	35.0	37.0
5	36.1757888790251	37.0	35.0	37.0	35.0	37.0
6	36.235073843918975	37.0	37.0	37.0	35.0	37.0
7	36.241562424340245	37.0	37.0	37.0	35.0	37.0
8	36.21932047453797	37.0	37.0	37.0	35.0	37.0
9	38.049600516503915	39.0	38.0	39.0	35.0	39.0
10	37.984876119764344	39.0	38.0	39.0	35.0	39.0
11	38.05750948268905	39.0	38.0	39.0	35.0	39.0
12	37.98053425873618	39.0	38.0	39.0	35.0	39.0
13	38.05111774675167	39.0	38.0	39.0	35.0	39.0
14	39.39607779840207	40.0	39.0	41.0	37.0	41.0
15	39.40664998789444	40.0	39.0	41.0	37.0	41.0
16	39.339084819627146	40.0	39.0	41.0	36.0	41.0
17	39.31597126946978	40.0	39.0	41.0	36.0	41.0
18	39.364474215156164	40.0	39.0	41.0	36.0	41.0
19	39.36047130982165	40.0	39.0	41.0	36.0	41.0
20	39.36563634896296	40.0	39.0	41.0	36.0	41.0
21	39.32497780647244	40.0	39.0	41.0	36.0	41.0
22	39.2906141554354	40.0	39.0	41.0	36.0	41.0
23	39.232346057622465	40.0	39.0	41.0	36.0	41.0
24	39.23755951900573	40.0	39.0	41.0	36.0	41.0
25	39.21020095230409	40.0	39.0	41.0	36.0	41.0
26	39.128900008070374	40.0	39.0	41.0	36.0	41.0
27	38.99933822936002	40.0	39.0	41.0	35.0	41.0
28	39.0078766846905	40.0	39.0	41.0	35.0	41.0
29	38.99121943345977	40.0	39.0	41.0	35.0	41.0
30	38.936340892583324	40.0	38.0	41.0	35.0	41.0
31	38.83973851989347	40.0	38.0	41.0	35.0	41.0
32	38.79622306512792	40.0	38.0	41.0	35.0	41.0
33	38.73480752158825	40.0	38.0	41.0	35.0	41.0
34	38.72919054152207	40.0	38.0	41.0	35.0	41.0
35	38.64133645387781	40.0	38.0	41.0	35.0	41.0
36	38.574077959809536	40.0	38.0	41.0	35.0	41.0
37	38.52392865789686	40.0	38.0	41.0	35.0	41.0
38	38.49857154386248	40.0	38.0	41.0	35.0	41.0
39	38.41596319909612	40.0	38.0	41.0	34.0	41.0
40	38.088467436042286	40.0	37.0	41.0	34.0	41.0
41	38.17601484948753	40.0	37.0	41.0	34.0	41.0
42	38.158001775482205	40.0	37.0	41.0	34.0	41.0
43	37.700104914857555	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	1.0
20	2.0
21	3.0
22	16.0
23	22.0
24	31.0
25	51.0
26	80.0
27	124.0
28	190.0
29	307.0
30	453.0
31	554.0
32	856.0
33	1080.0
34	1679.0
35	2458.0
36	3761.0
37	6585.0
38	15060.0
39	28640.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.557582116051975	16.705673472681784	12.744734081187959	28.992010330078283
2	21.833588895165846	19.18812040997498	32.06198046969575	26.916310225163425
3	20.66499878944395	19.480267936405454	30.105721894923736	29.749011379226857
4	15.046404648535228	17.104349931401824	35.26430473730934	32.58494068275361
5	17.278670002421112	31.957065612137843	33.1514809135663	17.61278347187475
6	31.05479783713986	34.823662335566134	16.37156000322815	17.749979824065854
7	29.122750383342748	27.93963360503591	20.731175853442014	22.206440158179326
8	26.398192236300538	29.907190702929547	20.939391493826164	22.75522556694375
9	29.841013638931486	12.12008716003551	18.035671051569686	40.00322814946332
10	20.41320313130498	22.259704624324105	28.113953676055203	29.21313856831571
11	39.927366637075295	19.199418933096602	18.003389556936487	22.869824872891613
12	22.81010410782019	24.91162940844161	25.53304818013074	26.74521830360746
13	36.75409571463158	16.954240981357437	20.146880800581066	26.14478250342991
14	23.570333306432087	21.375191671374385	23.623597772576872	31.430877249616657
15	30.717456218222907	24.02066015656525	19.72883544508111	25.53304818013074
16	24.538778145428132	24.243402469534338	22.9634412073279	28.254378177709626
17	27.736260188846746	23.693002986038252	20.84738923412154	27.723347590993463
18	27.534500847389232	20.095230409167943	22.74231296909047	29.627955774352355
19	28.9226051166169	22.027277862965057	23.33306432087806	25.717052699539988
20	31.364700185618595	19.35921233153095	22.863368573964973	26.41271890888548
21	27.476394157049473	21.66249697360988	21.95625857477201	28.90485029456864
22	29.17278670002421	23.249132434831733	20.19691711726253	27.381163747881526
23	28.141392946493422	20.916794447582923	22.12735049632798	28.814462109595674
24	28.12040997498184	21.144378984746997	23.110322007908966	27.624889032362198
25	27.708820918408524	21.28803163586474	23.373416189169557	27.62973125655718
26	28.362521184730856	22.95214268420628	21.89330966023727	26.792026470825597
27	26.930836897748367	21.71253329029134	25.119845048825763	26.236784763134537
28	26.58219675570979	22.722944072310547	21.63344362844	29.061415543539663
29	27.39407634573481	23.61875554838189	22.37753207973529	26.60963602614801
30	25.704140101686708	21.183116778306836	25.36034218384311	27.752400936163347
31	28.381890081510775	22.371075780808653	22.57444919699782	26.672584940682754
32	24.805100476152045	22.095069001694778	22.863368573964973	30.236461948188204
33	25.371640706964733	21.46719393107901	25.605681543055443	27.555483818900818
34	25.87361794851102	20.81833588895166	25.0698087321443	28.238237430393028
35	25.663788233395206	24.05455572593011	25.132757646679043	25.148898393995644
36	25.74772011944153	21.851343717214107	25.476555564522634	26.924380598821724
37	26.18029214752643	22.243563877007507	24.826083447663628	26.75006052780244
38	26.194818820111372	20.87967072875474	25.452344443547737	27.47316600758615
39	25.44911629408442	19.56581389718344	27.311758534420143	27.673311274312002
40	24.763134533128884	21.762569606972804	27.45056896134291	26.023726898555406
41	23.63812444516181	19.438301993382293	28.664353159551286	28.259220401904606
42	21.03300782826245	21.317084981034622	30.04600112985231	27.60390606085062
43	20.064562989266403	21.823904446775884	29.53111129045275	28.580421273504964
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	1.5
2	0.0
3	1.0
4	2.0
5	2.0
6	4.5
7	7.0
8	7.0
9	7.0
10	10.0
11	13.0
12	13.0
13	34.0
14	55.0
15	109.5
16	164.0
17	143.5
18	123.0
19	123.0
20	143.0
21	163.0
22	113.0
23	63.0
24	53.0
25	43.0
26	43.0
27	48.5
28	54.0
29	73.5
30	93.0
31	137.0
32	181.0
33	181.0
34	299.5
35	418.0
36	467.5
37	517.0
38	739.0
39	961.0
40	961.0
41	1232.0
42	1503.0
43	1968.5
44	2434.0
45	3736.5
46	5039.0
47	5039.0
48	5746.5
49	6454.0
50	7362.0
51	8270.0
52	8210.0
53	8150.0
54	8150.0
55	7218.5
56	6287.0
57	6127.5
58	5968.0
59	5494.0
60	5020.0
61	5020.0
62	4381.5
63	3743.0
64	2962.5
65	2182.0
66	1866.5
67	1551.0
68	1551.0
69	1192.0
70	833.0
71	735.5
72	638.0
73	502.0
74	366.0
75	366.0
76	286.5
77	207.0
78	181.5
79	156.0
80	135.0
81	114.0
82	114.0
83	95.5
84	77.0
85	69.0
86	61.0
87	44.0
88	27.0
89	27.0
90	17.5
91	8.0
92	4.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	61955.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.91162940844161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97753818508536	34.57186667742717
2	9.556154537286613	8.583649422968282
3	3.8778077268643303	5.224759906383666
4	2.238993710691824	4.022274231296909
5	1.4483378256963162	3.2523605842950527
6	1.0278526504941599	2.76975223952869
7	0.7259658580413297	2.2823016705673473
8	0.6109613656783468	2.195141635057703
9	0.431266846361186	1.743200710192882
>10	2.803234501347709	23.425066580582683
>50	0.2407906558849955	7.724961665725123
>100	0.06109613656783468	4.204664675974498
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA	230	0.37123718828181745	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA	193	0.31151642321039463	No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC	190	0.3066741990154144	No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA	189	0.30506012428375434	No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA	186	0.3002179000887741	No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG	181	0.2921475264304737	No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA	179	0.2889193769671536	No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT	147	0.23726898555403117	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT	138	0.22274231296909047	No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA	138	0.22274231296909047	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	138	0.22274231296909047	No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC	130	0.20982971511580983	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	128	0.2066015656524897	No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA	121	0.1953030425308692	No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA	106	0.17109192155596803	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	106	0.17109192155596803	No Hit
CATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCA	105	0.16947784682430797	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	98	0.15817932370268745	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	94	0.15172302477604713	No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG	94	0.15172302477604713	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	93	0.15010895004438704	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	93	0.15010895004438704	No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT	92	0.14849487531272698	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT	91	0.1468808005810669	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	89	0.14365265111774675	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	88	0.1420385763860867	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTCTT	86	0.13881042692276652	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	86	0.13881042692276652	No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA	86	0.13881042692276652	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	85	0.13719635219110643	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	85	0.13719635219110643	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	84	0.13558227745944637	No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT	84	0.13558227745944637	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	83	0.13396820272778628	No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGCTGTCTC	83	0.13396820272778628	No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA	83	0.13396820272778628	No Hit
CTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACAT	82	0.13235412799612623	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	82	0.13235412799612623	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.127511903801146	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	77	0.12428375433782585	No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC	77	0.12428375433782585	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	77	0.12428375433782585	No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC	77	0.12428375433782585	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	75	0.12105560487450569	No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC	75	0.12105560487450569	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	75	0.12105560487450569	No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC	74	0.11944153014284561	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	74	0.11944153014284561	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	73	0.11782745541118553	No Hit
CGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCG	73	0.11782745541118553	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	73	0.11782745541118553	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	72	0.11621338067952545	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	72	0.11621338067952545	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	71	0.11459930594786538	No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT	70	0.11298523121620531	No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA	69	0.11137115648454524	No Hit
AATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAA	67	0.10814300702122509	No Hit
GAAGAAGCACCGGCTAACTCCGTGCCAGCAGCCGCGGTAATAC	66	0.106528932289565	No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG	64	0.10330078282624484	No Hit
GTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCAG	64	0.10330078282624484	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	64	0.10330078282624484	No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC	63	0.10168670809458477	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	62	0.1000726333629247	No Hit
GCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCA	62	0.1000726333629247	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.001614074731660076	0.0
7	0.0	0.0	0.0	0.001614074731660076	0.0
8	0.0	0.0	0.0	0.001614074731660076	0.0
9	0.0	0.0	0.0	0.001614074731660076	0.0
10	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
11	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
12	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
13	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
14	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
15	0.001614074731660076	0.0	0.0	0.001614074731660076	0.0
16	0.003228149463320152	0.0	0.0	0.001614074731660076	0.0
17	0.003228149463320152	0.0	0.0	0.003228149463320152	0.0
18	0.003228149463320152	0.0	0.0	0.003228149463320152	0.0
19	0.003228149463320152	0.0	0.0	0.003228149463320152	0.0
20	0.003228149463320152	0.0	0.0	0.00807037365830038	0.0
21	0.003228149463320152	0.0	0.0	0.00807037365830038	0.0
22	0.003228149463320152	0.0	0.0	0.011298523121620531	0.0
23	0.003228149463320152	0.0	0.0	0.012912597853280607	0.0
24	0.003228149463320152	0.0	0.0	0.01614074731660076	0.0
25	0.003228149463320152	0.0	0.0	0.01614074731660076	0.0
26	0.003228149463320152	0.0	0.0	0.017754822048260833	0.0
27	0.003228149463320152	0.0	0.0	0.03389556936486159	0.0
28	0.003228149463320152	0.0	0.0	0.15010895004438707	0.0
29	0.003228149463320152	0.0	0.0	0.37285126301347754	0.0
30	0.003228149463320152	0.0	0.0	0.660156565248971	0.0
31	0.003228149463320152	0.0	0.0	1.315470906302962	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	130	0.0	31.307692	1
TAAACCA	30	3.560655E-4	30.833334	5
ACGTGTC	60	1.2205419E-9	30.833334	8
CCACGTG	55	1.7966158E-8	30.272728	6
ACCACGT	55	1.7966158E-8	30.272728	5
TACCACG	55	1.7966158E-8	30.272728	4
AAACCAT	25	0.0054567917	29.599998	6
ACCATGC	25	0.0054567917	29.599998	8
GCTAAAC	25	0.0054567917	29.599998	3
GTGTCCC	60	4.1856765E-8	27.750002	10
CGTGTCC	60	4.1856765E-8	27.750002	9
CTAAACC	40	5.8512785E-5	27.75	4
CGGTGCT	35	8.7746326E-4	26.428572	9
TGCGGGT	35	8.7746326E-4	26.428572	21
GGTGCTT	35	8.7746326E-4	26.428572	10
GTGCTTC	35	8.7746326E-4	26.428572	11
GGATACC	65	9.095493E-8	25.615385	1
GCCCTAC	70	1.8625906E-7	23.785715	17
CGCCCTA	70	1.8625906E-7	23.785715	16
CCGCCCT	70	1.8625906E-7	23.785715	15
>>END_MODULE