FastQCFastQC Report
Fri 10 Feb 2017
ERR1631632.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631632.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20015
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCCA690.3447414439170622No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAA580.28978266300274796No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCAC570.28478641019235573No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGACA520.2598051461403947No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAT510.2548088933300025No Hit
CATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGA490.24481638770921807No Hit
TCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACT450.22483137646764928No Hit
GTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCG450.22483137646764928No Hit
GTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAAC340.169872595553335No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAA340.169872595553335No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGCTGTCTCTT340.169872595553335No Hit
CCGTCATCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATA340.169872595553335No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA330.1648763427429428No Hit
CGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGATGA320.1598800899325506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT310.15488383712215836No Hit
GTGCGATTGCCTCGGTGATAACGACATTGGGGCCACTAAAGAT300.1498875843117662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT300.1498875843117662No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT290.14489133150137398No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA290.14489133150137398No Hit
CCGTCATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAAT280.13989507869098175No Hit
CCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTA280.13989507869098175No Hit
TTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCACTTCA270.13489882588058955No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG270.13489882588058955No Hit
TCTTTAGTGACCATGAAGTGCGATTGCCTCGGTGATAACGCTG270.13489882588058955No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTA270.13489882588058955No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA260.12990257307019734No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA260.12990257307019734No Hit
ACCATGAAGTGCGATTGCCTCGGTGATAACGACATTGGGGCTG260.12990257307019734No Hit
ATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAG260.12990257307019734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT260.12990257307019734No Hit
TTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCC230.11491381463902073No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT230.11491381463902073No Hit
ATCTTTAGTGGCCCCAATGTCGTTATCACCGAGGCAATCGCAC230.11491381463902073No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC230.11491381463902073No Hit
CTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGC230.11491381463902073No Hit
CTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCCTGTCTC220.10991756182862852No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG220.10991756182862852No Hit
CCCCAATGTCGTTATCACCGAGGCAATCGCACTTCATGGTCTG210.10492130901823633No Hit
GTCGTTATCACCGAGGCAATCGCACTTCATGGTCACTAAAGAC210.10492130901823633No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC210.10492130901823633No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC210.10492130901823633No Hit
CTTTACGCCCAGTAATTCCGATTAACGCTTGCACCCTCCGTAT210.10492130901823633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCC403.5215635E-837.09
GCGGTAA355.2718497E-737.023
ATACGGA200.001799037237.029
CGCGGTA355.2718497E-737.022
CGTGCCA403.5215635E-837.010
TAATACG307.910854E-637.027
TCCGTGC403.5215635E-837.08
AATACGG307.910854E-637.028
AGCCGCG403.5215635E-837.019
GTAATAC355.2718497E-737.026
CGGTAAT355.2718497E-737.024
CAGCAGC459.926589E-832.8888915
GCCAGCA459.926589E-832.8888913
AACTCCG459.926589E-832.888895
CTCCGTG459.926589E-832.888897
CCGCGGT459.926589E-832.8888921
GTGCCAG459.926589E-832.8888911
GGTAATA401.4986217E-632.37525
CGGAGGG250.0053697829.59999832
CCAGCAG502.502111E-729.59999814